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Method of measuring blood coagulation time to detect lupus anticoagulants

A lupus anticoagulant and blood coagulation technology, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, and biological testing, etc. Effect

Active Publication Date: 2014-03-26
SCHOOL JURIDICAL PERSON HIGASHI NIPPON GAKUEN +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, depending on the type of phospholipids (source, composition of phospholipids, etc.), the reaction intensity of LA varies, so depending on the reagent used for blood coagulation time measurement, the results of LA positive and negative judgments also differ.

Method used

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  • Method of measuring blood coagulation time to detect lupus anticoagulants
  • Method of measuring blood coagulation time to detect lupus anticoagulants
  • Method of measuring blood coagulation time to detect lupus anticoagulants

Examples

Experimental program
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Effect test

Embodiment 1

[0050] The plasma to be tested was diluted 1:1 with the auxiliary reagents listed in Table 1, and the obtained diluted reagent was used as a sample, and a normal mixing test was carried out, and the shapes of the curves in the figures were compared.

[0051]

[0052] (1) APTT screening test

[0053]The measurement was carried out using the PTT LA reagent "RD" (manufactured by Roche Diagnostics) and an automatic blood coagulation analyzer STA-R (manufactured by Roche Diagnostics). For determination, the cutoff value described in the manual attached to the reagent was used. This reagent is to use the PTT LA reagent containing cephalin as phospholipid, silicon dioxide as contact factor activator (negative charged body), and the reagent that other self-made calcium chloride solution is measured, can be with blood containing blood of the present invention. Buffer compositions (auxiliary reagents) combinations of coagulation factors are used in the method of the invention.

[00...

Embodiment 2

[0080] With respect to 9.5 volumes of the plasma to be tested, 0.5 volumes of an auxiliary reagent described later was added and mixed to prepare a measurement sample, and a dRVVT test was performed to obtain the ratio of coagulation time.

[0081]

[0082] (1) dRVVT test

[0083] As reagents for measuring blood coagulation time, DVVtest (registered trademark) and DVVconfirm (both manufactured by Sekisui Diagnostics Co., Ltd.) were used, and the blood coagulation automatic analyzer CP2000 (manufactured by SEKISUI MEDICAL Co., Ltd.) was used. The incubation time of the measurement sample was 216 seconds, APTT For freezing point parameters, the dRVVT test was performed. In this example, the cutoff value (t / c) was set to 1.3 or less, and LA was detected and judged. The reagent is composed of DVVtest (registered trademark) reagent containing Russell venom, phospholipid and calcium, and DVVconfirm reagent containing Russell venom, excess phospholipid and calcium, and can be comb...

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Abstract

Provided is a method of measuring blood coagulation time which makes it possible to detect lupus anticoagulants more easily and with a higher degree of sensitivity in comparison to the method recommended by the International Society on Thrombosis and Haemostasis (ISTH), and which is not affected by blood-coagulation-factor deficiencies, even in blood samples from patients on warfarin, patients with vitamin K deficiencies and patients with hepatic insufficiency. The method for measuring blood coagulation time to detect lupus anticoagulants is characterized in that a buffering solution composition containing blood coagulation factors is added to a blood sample both before and during the measurement of the blood coagulation time, and the blood coagulation time is measured.

Description

technical field [0001] The invention relates to a method for measuring blood coagulation time of a blood sample to be tested for the purpose of detecting lupus anticoagulant. Background technique [0002] Blood coagulation time measurement is to determine the blood coagulation factor activator (hereinafter, sometimes referred to as activator) and / or Ca 2+ The time when the reagent for measuring blood clotting time composed of other components is added to the sample blood or the sample blood mixture, and the time until a detectable fibrin clot is formed (blood clotting time: hereinafter, sometimes referred to simply as coagulation time. In addition, sometimes The formation of a fibrin clot is simply referred to as coagulation), which is carried out to screen for abnormalities in the blood coagulation system or to measure the activity of each blood coagulation factor. Typical examples include blood coagulation tests such as prothrombin time (PT), activated partial thromboplas...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/86
CPCC12Q1/56G01N2800/104G01N33/564G01N33/86G01N2800/24
Inventor 家子正裕森川千鹤服部惠子
Owner SCHOOL JURIDICAL PERSON HIGASHI NIPPON GAKUEN
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