A method for the cultivation of human tumor suppressor gene green fluorescent protein carrier, design primers on GENBANK with reference to human anti-cancer gene sequence, collect peripheral blood of early lung cancer patients to extract total RNA, and use reverse transcription polymerase chain reaction (RT-PCR) Amplify the P53 fragment, clone the gene fragment directionally onto the pMD18-T vector, construct the pMD18-P53 recombinant plasmid, identify the recombinant plasmid by PCR amplification, enzyme digestion and sequencing, and digest the pMD18-P53 Identify and extract the directional clone of the P53 target gene into the pEGFP-N1 eukaryotic expression vector to construct the pEGFP-N1-P53 expression vector. The beneficial effects of the present invention are: using the eukaryotic expression vector pEGFP-N1, which is safe, stable, strong in replication, high in expression rate, and easy to detect, as the transport carrier of human anti-cancer genes, thereby constructing a safe, stable, easy-to-detect, safe and reliable The eukaryotic expression vector pEGFP-N1-P53 is used for the prevention and treatment of cancer.