Lumbricus polypeptide having anticoagulant and thrombolytic effects, and enzymatic hydrolysis preparation method and application thereof

Abstract

The invention discloses a lumbricus polypeptide having anticoagulant and thrombolytic effects, and an enzymatic hydrolysis preparation method and an application thereof. The preparation method comprises the steps: cleaning and drying lumbricus dried bodies, and crushing into a powder; adding water, stirring, and centrifuging to obtain a protein precipitate; adding water to dissolve the protein, adding an alkaline protease with the ratio of the enzyme volume to the protein weight of 0.1-3 mL:100 g, and stirring; exterminating enzyme activity, adding trypsin with the ratio of the enzyme mass to the protein weight of 0.5-5 g:100 g, and stirring at the temperature of 25-60 DEG C; allowing the enzymatic hydrolysate to pass through an anion exchange column, collecting a part of eluate; and then passing through a macroporous adsorption resin, carrying out separation and purification, and collecting and drying to obtain a lumbricus anticoagulant peptide powder. A new source is added for high-activity anticoagulant active substances, the product activity is high, the preparation process is convenient and feasible, and the environment is protected; and the lumbricus polypeptide can be used as a raw material of foods for thrombosis prevention, thrombolysis and blood consistency reduction, is supplemented with lactose, mannitol and the like, and is made into a powder, a tablet, a capsule or an oral liquid.

Description

technical field [0001] The present invention relates to a biological peptide and its preparation method and application, in particular to an anticoagulant and thrombus-dissolving earthworm polypeptide and its enzymatic preparation method and application. Background technique [0002] Dilong, that is, earthworm, is cold in nature and slightly salty in taste. It is a traditional Chinese medicinal material in my country. According to records, more than 2,000 years ago, our ancestors used earthworm to treat qi deficiency and blood stagnation, stroke, hemiplegia and other diseases caused by vein stasis. "Compendium of Materia Medica" lists more than 40 kinds of earthworm prescriptions. Modern pharmacological research has found that it not only anticoagulant, inhibits platelet aggregation, antiarrhythmia, but also has anticancer and analgesic effects. Most of these functional ingredients are extracted by decoction or powdered orally directly. The utilization rate of the former is...

AI Technical Summary

Problems solved by technology

Most of these functional ingredients are extracted by decoction or powdered orally directly. The utilization rate of the former is low, and the dosage of the latter is large, and the activity after entering the human body is not high.

Some people extract a single protein from Earthworm to make preparations, but the oral preparations are easily digested by gastric enzymes and pancreatin after passing through the gastrointestinal tract, and the activity is greatly reduced; and when made into injections, macromolecular proteins are also It is easy to cause immunogenicity and has potential safety hazards

The functional substances obtained by these two methods are very complex, and the molecular weight is mostly in the range of 12,000 to 80,000, which affects their clinical application and drug efficacy research tracking to a certain extent.

[0003] There are freeze-dried earthworm powder supplemented with other Chinese medicinal materials for the treatment of cardiovascular and cerebrovascular diseases on the market, and there has not yet been a functional product that uses the active small peptides obtained by enzymatic hydrolysis of earthworm proteolysis to make anticoagulant and thrombolytic products. The development of a low-cost, high-activity Dilong product has broad market economic benefits

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