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Lupus anticoagulant confirmation reagent and preparation method thereof

A lupus anticoagulant and reagent technology, applied in the biological field, can solve problems such as poor stability and interference with the accuracy of test results, and achieve the effects of good stability, strong anti-interference ability, and reduced reagent waste

Active Publication Date: 2021-03-26
BEIJING MDC NEW SPRING MEDICAL DEVICES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The existing lupus anticoagulant confirmation reagent has the problem of poor stability, and the hemoglobin, bilirubin, triglyceride, and heparin in the specimen will interfere with the accuracy of the test result

Method used

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  • Lupus anticoagulant confirmation reagent and preparation method thereof
  • Lupus anticoagulant confirmation reagent and preparation method thereof
  • Lupus anticoagulant confirmation reagent and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Weigh 2 g of tris(hydroxymethyl)aminomethane and dissolve it in 800 mL of deionized water, stir well for 15-20 min, then add 80 U of coagulation factor X activator, 10 mg of rabbit cephalin, 20 g of gelatin, 35 g of glycine, 15 g of trehalose, and 0.1 g of thimerosal sodium. , 1.2g of calcium chloride, stir for 20-50min, dilute the volume to 1 liter, adjust the pH to 8.0, the solution is the lupus anticoagulant confirmation detection reagent (LAC).

Embodiment 2

[0050]Weigh 3g of trishydroxymethylaminomethane and dissolve it in 800mL deionized water, stir well for 15-20min, then add 90U coagulation factor X activator, 12mg rabbit cephalin, 22g gelatin, 37g glycine, 10g trehalose, 0.3g sodium thimerosal , 1.5 g of calcium chloride, stirred for 20-50 min, set the volume to 1 liter, and adjusted the pH to 8.0. This solution was the Lupus Anticoagulant Confirmation Test Reagent (LAC).

Embodiment 3

[0052] Weigh 5g of trishydroxymethylaminomethane and dissolve it in 800mL of deionized water, stir well for 15-20min, then add 100U of coagulation factor X activator, 15mg of rabbit cephalin, 25g of gelatin, 40g of glycine, 14g of trehalose, and 0.5g of sodium thimerosal , calcium chloride 1.8g, stirring for 20-50min, set the volume to 1 liter, adjust the pH to 8.0, the liquid is the lupus anticoagulant confirmation test reagent (LAC).

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Abstract

The invention relates to the technical field of biology, in particular to a lupus anticoagulant confirmation reagent and a preparation method thereof. The confirmation reagent comprises tris(hydroxymethyl) aminomethane, a blood coagulation factor X activator, rabbit cephalin, gelatin, glycine, trehalose, sodium merthiolate and calcium chloride. According to the lupus anticoagulant confirmation kit, by adjusting the concentration and components of the stabilizer, the lupus anticoagulant confirmation kit is good in stability and high in interference resistance, the reagent can be placed stably for 10 days after being redissolved at the temperature of 2-8 DEG C, and the freeze-dried powder can be placed stably for 2 years at the temperature of 2-8 DEG C. The stability of the reagent is superior to that of common commercially available products, reagents are fully utilized, reagent waste is reduced, and cost is well saved for hospitals.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a confirmation reagent for lupus anticoagulant and a preparation method thereof. Background technique [0002] Lupus anticoagulant (LA) is a pathological circulating anticoagulant, which is IgG, IgM or a mixture of both. LA binds to prothrombin by recognizing phospholipids, blocks the interaction between activated coagulation factor V and prothrombin, inhibits the formation of fibrin, and interferes with APTT, PT, and dRVVT coagulation tests in vitro, resulting in prolonged coagulation time. [0003] In vivo, LA can activate platelets and / or bind to β2-GPI, induce adhesion molecules, tissue factor expression and complement activation to generate a prothrombotic state and promote thrombosis. LA interacts with vascular endothelial cell membrane phospholipids, interferes with endothelial cells to release plasminogen activator and inhibits fibrinolysis; LA interacts with platelet membra...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/573
CPCG01N33/6854G01N33/573G01N2333/974G01N2333/96444Y02A50/30
Inventor 隋延峰胡军邹红建
Owner BEIJING MDC NEW SPRING MEDICAL DEVICES
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