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Method for detecting lupus anticoagulants

A lupus anticoagulant, detection method technology, applied in biochemical equipment and methods, microbial determination/inspection, biological testing, etc., to achieve the effect of eliminating the difference between batches

Active Publication Date: 2014-03-19
SCHOOL JURIDICAL PERSON HIGASHI NIPPON GAKUEN +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Therefore, these LA detection methods are seldom used alone, and it is recommended to combine two or more inspections and judge the results comprehensively (Non-Patent Document 3)

Method used

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  • Method for detecting lupus anticoagulants
  • Method for detecting lupus anticoagulants
  • Method for detecting lupus anticoagulants

Examples

Experimental program
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Effect test

Embodiment

[0040] The present invention is further described in detail through the following examples, but the present invention is not limited to the following examples.

[0041]

[0042] (1) APTT screening test

[0043] Measurement was performed using PTT LA reagent "RD" (manufactured by DIAGNOSTICA STAGO) and an automatic blood coagulation analyzer STA-R (manufactured by Roche Diagnostics). For the cutoff value used for determination, a value obtained by measuring the value of 20 or more healthy subjects + 2SD was used.

[0044] (2) dRVVT test

[0045] The LA test "Gradipore" (manufactured by the Institute of Medical Biology) was used, and the blood coagulation automatic analyzer STA-R was used for measurement. For the cutoff value used for determination, a value obtained by measuring the value of 20 or more healthy subjects + 2SD was used.

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Abstract

Provided is a simple method for detecting lupus anticoagulants without using blood plasma from healthy individuals, which is capable of distinguishing between blood-coagulation-factor deficiencies, even in blood samples originating from patients undergoing anticoagulant therapies using warfarin, heparin, or the like, without being affected by said anticoagulant therapies. The method for detecting the lupus anticoagulants is characterized in that it involves the following three steps, (A), (B) and (C): (A) a step in which a buffering solution composition containing blood coagulation factors is added to a blood sample and to a diluted sample of said blood sample both before and during the measurement of the blood coagulation time; (B) a step in which the blood coagulation time is measured for each of the samples from step (A); and (C) a step in which the blood coagulation times of each of the samples obtained in step (B) are compared.

Description

technical field [0001] The invention relates to a method for detecting positive lupus anticoagulant used in the diagnosis of antiphospholipid antibody syndrome. Background technique [0002] Lupus anticoagulant (LA) is the first reported circulating anticoagulant in patients with SLE (Systemic Lupus Erythematosus). For LA-positive patients, little bleeding tendency was clinically recognized, but a thrombus tendency was shown. However, samples derived from LA-positive patients showed a tendency to prolong activated partial thromboplastin time (APTT) and prothrombin time (PT) in vitro. According to subsequent studies, it was clarified that LA is an autoantibody against a complex of negatively charged phospholipids and proteins in the blood such as β2-glycoprotein I (β2GPI) or prothrombin, and it is now known that LA is an autoantibody against diseases other than SLE. The disease also mostly detects LA. In particular, the occurrence frequency is high in the pathology collect...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/86
CPCC12Q1/56G01N2800/104G01N33/86G01N33/564G01N2800/24
Inventor 家子正裕森川千鹤
Owner SCHOOL JURIDICAL PERSON HIGASHI NIPPON GAKUEN
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