The invention discloses a nucleic acid composition, kit and detection method for detecting fusion mutation of a human FGFR2 (fibroblast growth factor receptor 2) gene. The nucleic acid composition comprises primers and probes designed for 18 fusion mutation types of the FGFR2 gene. The kit comprises the nucleic acid composition, and also comprises DNA polymerase, a PCR buffer solution, dNTPs and cations. The invention also provides a method for detecting fusion mutation of the FGFR2 gene. The method comprises the following steps: firstly, extracting RNA of a detection sample, carrying out reverse transcription to obtain cDNA, carrying out real-time fluorescent PCR reaction by using the kit with the cDNA as a template, and finally judging the negative and positive properties of the detection sample according to a Ct value. The kit can be used for simultaneously and specifically detecting 18 fusion mutations of the FGFR2 gene, is wide in mutation site coverage range, reduces the detection times, and has the advantages of high sensitivity, good repeatability, simplicity and convenience in operation, high detection speed, easiness in result interpretation and the like.