Novel splice variant of FGFR3 (Fibroblast Growth Factor Receptor 3) in liver carcinoma tissue and application thereof

A liver cancer tissue and variant technology, applied in the biological field, can solve the problems of lack of effective molecular markers and hysteresis, and achieve high guiding significance, promote growth, promote proliferation and metastasis

Active Publication Date: 2016-02-03
RUIJIN HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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Problems solved by technology

In terms of clinical diagnosis and treatment, my country currently lacks effective, specific, and guiding molecular markers, as well as corresponding diagnostic, treatment, and prognostic detection methods, especially in the research of specific drugs.

Method used

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  • Novel splice variant of FGFR3 (Fibroblast Growth Factor Receptor 3) in liver carcinoma tissue and application thereof
  • Novel splice variant of FGFR3 (Fibroblast Growth Factor Receptor 3) in liver carcinoma tissue and application thereof
  • Novel splice variant of FGFR3 (Fibroblast Growth Factor Receptor 3) in liver carcinoma tissue and application thereof

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Embodiment Construction

[0019] The technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only some of the embodiments of the present invention, but not all of them. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without creative efforts fall within the protection scope of the present invention.

[0020] 1. RNA extraction from liver cancer tissue

[0021] Using Trizol reagent for extraction, the specific steps are as follows:

[0022] (1) Take out clinical liver cancer tissue specimens and other normal control specimen blocks from the refrigerator at -70°C, take out 50 mg and add it to a glass homogenizer that has been soaked in Depc water and subjected to high temperature and high pressure, and quickly add 1ml Trizol reagent to the homogenizer, Manual grinding, all operations must be done on ice, and the grinding time of each sp...

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Abstract

The invention discloses a novel splice variant of FGFR3 (Fibroblast Growth Factor Receptor 3) in liver carcinoma tissue and application thereof. The novel splice variant is FGFR3 delta7-9, and the gene sequence of FGFR3 delta7-9 is CGCTGGACGTGCTGGCCGAGGAGGAGCTGG. According to the novel splice variant FGFR3 delta7-9, proliferation and transfer of hepatoma carcinoma cells are promoted, the growth of subcutaneous hepatoma carcinoma cell transplantation tumor of a naked mouse is further promoted; besides, the experimental result shows that FGFR3 delta7-9 can serve as the diagnostic marker of liver cancer, therefore whether the splice variant FGFR3 delta7-9 exists in the liver carcinoma tissues of patients has higher guiding significance on the judgment of prognosis in liver cancer.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a novel splicing variant of FGFR3 in liver cancer tissue and its application. Background technique [0002] Chronic liver disease is one of the major public health problems that endanger the health of our people. It ranks sixth among the main causes of death in our country. The carrier rate of hepatitis B virus HBV in the population is 9.09%, of which about 5000 are chronic hepatitis B patients. Ten thousand, recurrent attacks of liver inflammation lead to 10% to 20% of patients developing liver cirrhosis, of which 10% to 15% of patients with liver cirrhosis eventually develop into hepatocellular carcinoma. In addition to the hazards of liver cancer, each stage in the transformation process of hepatitis-cirrhosis-hepatocarcinoma will cause serious harm to the health and life of patients, and has caused a heavy economic burden and social problems in our country. The current research ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/71C12N15/12C12N15/10G01N33/68G01N33/574
Inventor 邱伟华沈柏用彭承宏杨卫平李科张贵阳景晓乾
Owner RUIJIN HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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