Tissue dewaxing transparent agent free of benzene

A clearing agent and dewaxing technology, used in the field of tissue dewaxing clearing agent and tissue dewaxing preparations, can solve problems such as inability to achieve clearing effect, and achieve the effect of good clearing effect, improved integrity rate, and not easy to shrink and deform

Inactive Publication Date: 2014-11-19
陆可望 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The refractive index of animal tissue is 1.418, while the refractive index of xylene is 1.49, and the refractive index of benzene and toluene is 1.50, which cannot achieve the best transparent effect.
At present, there is no product that can make tissue transparent and dewax, completely replace benzene preparations to achieve the best effect

Method used

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  • Tissue dewaxing transparent agent free of benzene
  • Tissue dewaxing transparent agent free of benzene
  • Tissue dewaxing transparent agent free of benzene

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Example 1, Mix the ingredients of the formula evenly and dissolve it (the liquid is based on the volume ratio, and the dibutyl hydroxytoluene is based on the mass volume percentage).

[0053] formula 1 2 3 4 5 6 7 Cyclohexane 100.0 0.0 0.0 0.0 0.0 0.0 50.0 Methylcyclohexane 0.0 100.0 0.0 100.0 50.0 50.0 50.0 Dicyclohexane 100.0 50.0 50.0 essential oil 0.0 0.0 0.0 5.0 0.0 1.0 0.0 amphoteric surfactant 0.0 0.0 0.0 0.0 0.0 0.1 0.0 Butylated hydroxytoluene 0.0 0.0 0.05 0.05 0.0 0.05 0.0

Embodiment 2

[0055] Cavity tissue (lung) transparency, dewaxing test and results

[0056] Material: formula 1, formula 2 in embodiment 1

[0057] experimental method:

[0058] Experimental Group 1 (Recipe 1)

[0059] ①. Take the lung tissue of the dog, fix and dehydrate according to the routine tissue fixation and alcohol gradient dehydration operation steps, and then use Recipe 1 The reagents were divided into two tanks for tissue clearing, and the clearing time of each tank was 10 minutes for the first tank and 5 minutes for the second tank. Finally, the standard paraffin embedding procedure was used for tissue embedding.

[0060] ②. Slice and spread in warm water.

[0061] ③. Put Formula 1 in two glass jars, and put the glass slides with tissue sections into the two glass jars for dewaxing respectively, each jar for 5 minutes.

[0062] ④. Take out the slide, carry out alcohol gradient elution according to the standard operation, and carry out routine HE staining.

[0063]...

Embodiment 3

[0078] For dense tissue (liver) clearing, dewaxing test and results

[0079] Material: Formulation 3 in Example 1

[0080] experimental method:

[0081] test group:

[0082] ①. Rabbit liver tissue , fix and dehydrate according to the routine tissue fixation and alcohol gradient dehydration steps, and then use Recipe 3 The reagents were divided into two tanks for tissue clearing, and the clearing time of each tank was 20 minutes. Finally, the standard paraffin embedding procedure was used for tissue embedding.

[0083] ②. Slice and spread in warm water.

[0084] ③. Will Recipe 3 Place in two glass jars, and put the glass slides carrying the tissue sections into the two glass jars for dewaxing for 10 minutes respectively.

[0085] ④. Take out the slide, carry out alcohol gradient elution according to the standard operation, and carry out routine HE staining.

[0086] ⑤ with Recipe 3 Take out one cylinder after 30 seconds of transparency, air dry and seal the s...

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Abstract

The invention relates to a tissue dewaxing transparent agent free of benzene, which is used in biological histology, histopathology or forensic science, can make a tissue transparent, and can make the tissue dewaxed. The tissue dewaxing transparent agent belongs to the technical field of in vitro diagnostic reagents. The tissue dewaxing transparent agent mainly contains 95%-100% of alicyclic hydrocarbon, and the balance of accessories; the alicyclic hydrocarbon is a monocyclic alicyclic hydrocarbon or a bicyclic alicyclic hydrocarbon. The tissue dewaxing transparent agent free of benzene has the advantages of being non-toxic, soft in effect, and not easy to cause shrinkage, deformation, hardening and embrittlement of a tissue material, capable of improving the section intact rate, insensitive to the humid environment, not easy to muddy due to absorption of moisture in the air, and the like; not only can be used for liquid based cytology, immunohistochemistry, biological histology, routine pathological diagnosis, and immunohistochemical diagnostic techniques, is also suitable for special staining method and histochemical techniques. The refractive index of used alicyclic hydrocarbon transparent agent (cyclohexane) is 1.42, the (methyl cyclohexane) refractive rate is 1.42, and is basically consistent with the tissue refractive index (1.418), and the transparent effect is better.

Description

technical field [0001] The present invention relates to a benzene-free tissue dewaxing and clearing agent used in biological histology, histopathology and forensic medicine. . It belongs to the technical field of in vitro diagnostic reagents. Background technique [0002] Clinical laboratories are playing an increasingly important role in clinical medicine. The diagnostic information provided by clinical laboratories involves diagnosis, treatment, health status assessment, public health emergencies, and scientific research. It accounts for more than 60% of all medical information such as diagnosis and curative effect of patients. [0003] The birth of histopathology has a history of more than 150 years. Tissue paraffin section is the most commonly used sectioning technique in qualitative analysis such as observation of tissue morphology and immunohistochemistry, and is widely used in clinical pathological diagnosis and experimental research. After the tissue is fixed and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28G01N1/30
Inventor 陆可望张磊
Owner 陆可望
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