Tissue dewaxing transparent agent free of benzene
A clearing agent and dewaxing technology, used in the field of tissue dewaxing clearing agent and tissue dewaxing preparations, can solve problems such as inability to achieve clearing effect, and achieve the effect of good clearing effect, improved integrity rate, and not easy to shrink and deform
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Embodiment 1
[0052] Example 1, Mix the ingredients of the formula evenly and dissolve it (the liquid is based on the volume ratio, and the dibutyl hydroxytoluene is based on the mass volume percentage).
[0053] formula 1 2 3 4 5 6 7 Cyclohexane 100.0 0.0 0.0 0.0 0.0 0.0 50.0 Methylcyclohexane 0.0 100.0 0.0 100.0 50.0 50.0 50.0 Dicyclohexane 100.0 50.0 50.0 essential oil 0.0 0.0 0.0 5.0 0.0 1.0 0.0 amphoteric surfactant 0.0 0.0 0.0 0.0 0.0 0.1 0.0 Butylated hydroxytoluene 0.0 0.0 0.05 0.05 0.0 0.05 0.0
Embodiment 2
[0055] Cavity tissue (lung) transparency, dewaxing test and results
[0056] Material: formula 1, formula 2 in embodiment 1
[0057] experimental method:
[0058] Experimental Group 1 (Recipe 1)
[0059] ①. Take the lung tissue of the dog, fix and dehydrate according to the routine tissue fixation and alcohol gradient dehydration operation steps, and then use Recipe 1 The reagents were divided into two tanks for tissue clearing, and the clearing time of each tank was 10 minutes for the first tank and 5 minutes for the second tank. Finally, the standard paraffin embedding procedure was used for tissue embedding.
[0060] ②. Slice and spread in warm water.
[0061] ③. Put Formula 1 in two glass jars, and put the glass slides with tissue sections into the two glass jars for dewaxing respectively, each jar for 5 minutes.
[0062] ④. Take out the slide, carry out alcohol gradient elution according to the standard operation, and carry out routine HE staining.
[0063]...
Embodiment 3
[0078] For dense tissue (liver) clearing, dewaxing test and results
[0079] Material: Formulation 3 in Example 1
[0080] experimental method:
[0081] test group:
[0082] ①. Rabbit liver tissue , fix and dehydrate according to the routine tissue fixation and alcohol gradient dehydration steps, and then use Recipe 3 The reagents were divided into two tanks for tissue clearing, and the clearing time of each tank was 20 minutes. Finally, the standard paraffin embedding procedure was used for tissue embedding.
[0083] ②. Slice and spread in warm water.
[0084] ③. Will Recipe 3 Place in two glass jars, and put the glass slides carrying the tissue sections into the two glass jars for dewaxing for 10 minutes respectively.
[0085] ④. Take out the slide, carry out alcohol gradient elution according to the standard operation, and carry out routine HE staining.
[0086] ⑤ with Recipe 3 Take out one cylinder after 30 seconds of transparency, air dry and seal the s...
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