39 results about "Immunoglobulin IgG" patented technology

The present invention relates to the field of biomedicine, and specifically provides a human interleukin 10-Fc fusion protein, which is formed by fusing human interleukin 10 with an Fc fragment of immunoglobulin IgG through a connecting peptide; wherein, The Fc fragment is selected from one of human IgG1, IgG2 or IgG4; the amino acid sequence of the human interleukin 10 is shown in SEQ ID No:1. The present invention is formed by fusion of human IL-10 and human immunoglobulin Fc fragment through a specific connecting peptide, which prolongs the half-life of human IL-10 in vivo and increases its stability in vivo. In addition, the present invention provides The human interleukin 10-Fc fusion protein can be used for the treatment of immune diseases and cancer.

The invention discloses a silicon-based rhodamine fluorescent dyeing reagent as well as its preparation method and application, belonging to the fields of organic chemistry and biochemistry. The present invention uses the rhodamine derivatives substituted with silicon atoms as the basic skeleton, and synthesizes a silicon-based rhodamine fluorescent substance with a large Stokes shift and high fluorescence intensity capable of labeling immunoglobulin IgG by modifying different forms of aromatic amines. The staining reagent can be used for in vitro SARS-CoV-2 virus-specific antibody fluorescent ELISA detection. The silicon-based rhodamine fluorescent staining reagent of the present invention has a large Stokes shift (>140nm), can effectively avoid the mutual interference of excitation and emission light, and has high detection sensitivity, and the fluorescent ELISA detection method established based on the fluorescent antibody can be applied Microplate readers with different bandwidths have a wide range of applications.

The invention relates to a method for separating immune globulin IgG from human serum. The method comprises the following steps: 1) diluting the human serum by a buffer solution, adjusting a pH to 6.0to 8.0 and filtering by a filter membrane to obtain a human serum sample; 2) feeding the human serum sample into a chromatographic column, washing by a balancing buffer solution, then eluting by a eluting buffer solution and collecting eluent to obtain the immune globulin IgG solution, the chromatographic column is filled with a combined type bionic chromatography medium, the combined type bionicchromatography medium is prepared from a chromatography matrix and combined type ligand, the chromatography matrix is a hydrophilic porous microsphere with hydroxyl, and a sequence of the combined type ligand is phenylalanine-tyrosine-glutamine-5-aminobenzimidazole; 3) performing utlrafiltration and concentration on the immune globulin IgG solution to obtain the immune globulin IgG. The method can improve an efficiency and a purity for separating the immune globulin IgG from blood.

The invention relates to a screening method for a hybridoma cell line able to secrete an anti-bovine immunoglobulin IgG monoclonal antibody for bovine immunoglobulin IgG detection, the hybridoma cell line, the monoclonal antibody secreted thereby and application in the field of bovine immunoglobulin IgG detection. The hybridoma cell is preserved in China Center For Type Culture Collection, Wuhan University at Luojiashan, Wuchang, Wuhan City in Hubei Province, and the preservation number is CCTCC No:2013183. The anti-bovine immunoglobulin IgG monoclonal antibody secreted by the hybridoma cell has the advantages of strong specificity, large affinity and high titer and the like, and can be widely applied in the detection reagent or detection equipment field of bovine immunoglobulin IgG. A bovine immunoglobulin IgG rapid detection card built based on the immunochromatography principle can be used for detection of bovine immunoglobulin IgG in colostrum and its products, bovine blood and other samples, and has significant advantages compared with conventional detection methods in the aspects of specificity, sensitivity and detection efficiency, etc.

The invention discloses a staphylococcus protein A, a purification preparation method and application thereof. The invention mainly relates to the construction of a recombinant plasmid by linking a genetically engineered protein ProteinA gene into an expression vector, and then introducing it into a prokaryotic host cell for expression. And and ion exchange chromatography purification, can obtain the target protein with a purity of more than 95%. The purification preparation method of the invention has the characteristics of simple purification process and high yield. At the same time, the prepared ProteinA has good immunoglobulin IgG binding ability, alkali resistance and thermal stability. In addition, the affinity medium prepared by the solid-phase carrier coupled with ProteinA has the advantages of good IgG adsorption effect, high sample loading capacity and alkali resistance. The ProteinA protein prepared by the invention and the medium prepared by coupling can play an important role in the field of monoclonal antibody purification and immunodiagnosis research.