Hybridoma cell line capable of secreting monoclonal antibody against bovine immunoglobulin IgG and its application

A hybridoma cell line and immunoglobulin technology, which is applied in the field of bovine immunoglobulin IgG immunodetection, can solve the problems of complicated operation, complicated equipment and time-consuming, and achieve the effect of good specificity and high sensitivity
CN104099300BInactive Publication Date: 2017-03-08SHENYANG AGRI UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
SHENYANG AGRI UNIV
Publication Date
2017-03-08
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to a screening method for a hybridoma cell line able to secrete an anti-bovine immunoglobulin IgG monoclonal antibody for bovine immunoglobulin IgG detection, the hybridoma cell line, the monoclonal antibody secreted thereby and application in the field of bovine immunoglobulin IgG detection. The hybridoma cell is preserved in China Center For Type Culture Collection, Wuhan University at Luojiashan, Wuchang, Wuhan City in Hubei Province, and the preservation number is CCTCC No:2013183. The anti-bovine immunoglobulin IgG monoclonal antibody secreted by the hybridoma cell has the advantages of strong specificity, large affinity and high titer and the like, and can be widely applied in the detection reagent or detection equipment field of bovine immunoglobulin IgG. A bovine immunoglobulin IgG rapid detection card built based on the immunochromatography principle can be used for detection of bovine immunoglobulin IgG in colostrum and its products, bovine blood and other samples, and has significant advantages compared with conventional detection methods in the aspects of specificity, sensitivity and detection efficiency, etc.
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Description

technical field

[0001] The invention relates to the field of bovine immunoglobulin IgG immunodetection, in particular to a hybridoma cell line 9C6 capable of secreting anti-bovine immunoglobulin IgG monoclonal antibody and an application thereof. Background technique

[0002] The bovine immunoglobulin system is similar to other mammals in terms of physical and chemical properties and classification and naming, mainly including IgG, IgA and IgM. IgG is divided into two subclasses, IgG1 and IgG2, of which IgG1 is the main component. IgG molecule is composed of 4 peptide chains, 2 long chains are called heavy chain (heavy chain, H), composed of about 440 amino acid residues, molecular weight is about 50-70kD; 2 short chains are called light chain (light chain) , L), consisting of about 220 amino acids, with a molecular weight of about 22.5kD. The four peptide chains are linked together by interchain disulfide bonds (-S·S-). Bovine immunoglobulin is widely distributed in bovi...

Claims

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