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High performance liquid chromatography detection method for immunoglobulin igG in cow milk

A high-performance liquid chromatography and immunoglobulin technology, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of increasing detection operation cost and difficulty and cost, difficulty in purchasing rabbit anti-bovine IgG antibody powder, cumbersome operation steps, etc. , to achieve the effects of high penetration rate, simplified pre-processing steps, and high detection accuracy

Pending Publication Date: 2021-03-09
河南省奶牛生产性能测定有限公司 +2
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] The current standard method for detecting immunoglobulin IgG in milk is only the spectrophotometer method, "NY / T 2070-2011 Spectrophotometric method for the determination of immunoglobulin IgG in milk and its products", although this method can meet the time detection tasks However, the operation steps are cumbersome, the detection efficiency is low, and the rabbit anti-bovine IgG antibody powder is difficult to buy in the market, which further increases the cost and difficulty of the detection operation.

Method used

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  • High performance liquid chromatography detection method for immunoglobulin igG in cow milk
  • High performance liquid chromatography detection method for immunoglobulin igG in cow milk

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Such as figure 1 Shown, a kind of immunoglobulin igG high performance liquid chromatography detection method in bovine milk comprises the steps:

[0018] S1, sample prefabrication, first accurately weigh the milk to be tested, and add sodium phosphate buffer to the milk for dilution, then centrifuge the diluted milk through a centrifuge and let it stand, finally take the supernatant after standing and After the clear liquid is filtered through an ionic membrane, the finished product is obtained and set aside;

[0019] S2, chromatographic column detection, first select the chromatographic column—Bio Protein G column, and adjust the chromatographic column—Bio Protein G column to 10°C at a constant speed, then take 8 μL of the sample prepared in step S1, and uniformly speed at 0.3mL / min Add to the chromatographic column—Bio Protein G column, and then add a concentration of 50mmol / L sodium phosphate buffer (pH7.4) and a concentration of 0.5mol / L acetic acid to the chromato...

Embodiment 2

[0023] Such as figure 1 Shown, a kind of immunoglobulin igG high performance liquid chromatography detection method in bovine milk comprises the steps:

[0024] S1, sample prefabrication, first accurately weigh the milk to be tested, and add sodium phosphate buffer to the milk for dilution, then centrifuge the diluted milk through a centrifuge and let it stand, finally take the supernatant after standing and After the clear liquid is filtered through an ionic membrane, the finished product is obtained and set aside;

[0025] S2, chromatographic column detection, first select the chromatographic column—Bio Protein G column, and adjust the chromatographic column—Bio Protein G column to 45°C at a constant speed, then take 15 μL of the sample prepared in step S1, and speed it at a constant speed of 0.6mL / min Add to the chromatographic column—Bio Protein G column, and then add a concentration of 50mmol / L sodium phosphate buffer (pH7.4) and a concentration of 0.5mol / L acetic acid t...

Embodiment 3

[0029] Such as figure 1 Shown, a kind of immunoglobulin igG high performance liquid chromatography detection method in bovine milk comprises the steps:

[0030] S1, sample prefabrication, first accurately weigh the milk to be tested, and add sodium phosphate buffer to the milk for dilution, then centrifuge the diluted milk through a centrifuge and let it stand, finally take the supernatant after standing and After the clear liquid is filtered through an ionic membrane, the finished product is obtained and set aside;

[0031] S2, chromatographic column detection, first select the chromatographic column—Bio Protein G column, and adjust the chromatographic column—Bio Protein G column to 25°C at a constant speed, then take 5 μL of the sample prepared in step S1, and uniformly speed at 0.5mL / min Add to the chromatographic column—Bio Protein G column, and then add a concentration of 50mmol / L sodium phosphate buffer (pH7.4) and a concentration of 0.5mol / L acetic acid to the chromato...

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Abstract

The invention provides a high performance liquid chromatography detection method for immunoglobulin igG in cow milk. The high performance liquid chromatography detection method comprises two steps ofsample prefabrication, chromatographic column detection and the like. The brand-new method for determining the immunoglobulin IgG in the cow milk is established, the variety and the type of the methodfor detecting the immunoglobulin IgG in the cow milk are effectively expanded, the detection precision is high, more choices are provided for the detection field, and a liquid chromatograph adopted by the method is high in popularity rate and high in sensitivity. The instrument is good in running stability and easy to operate, and meanwhile, the pretreatment step of the detection sample can be effectively simplified, so that the detection efficiency is improved.

Description

technical field [0001] The invention relates to a high-performance liquid chromatography detection method for immunoglobulin igG in milk, belonging to the technical field of microbial detection. Background technique [0002] The current standard method for detecting immunoglobulin IgG in milk is only the spectrophotometer method, "NY / T 2070-2011 Spectrophotometric method for the determination of immunoglobulin IgG in milk and its products", although this method can meet the time detection tasks However, the operation steps are cumbersome, the detection efficiency is low, and the rabbit anti-bovine IgG antibody powder is difficult to buy in the market, which further increases the cost and difficulty of the detection operation. [0003] Therefore, in view of this situation, it is necessary to develop a new method for the detection of immunoglobulin in milk to meet the needs of practical use. Contents of the invention [0004] The object of the present invention is to provid...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/34G01N30/36G01N30/60G01N30/88
CPCG01N30/02G01N30/06G01N30/34G01N30/36G01N30/60G01N30/88G01N2030/8831
Inventor 闫磊张震高娜任小丽皇超英闫跃飞岳婷婷薛永康李静茹刘欢潘磊侯玉芳魏成斌邓红雨马平安
Owner 河南省奶牛生产性能测定有限公司
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