Preparation method of microcapsule with function of immune isolation

A technology of microcapsules and preparation methods, applied in microcapsule preparations, microsphere preparation and other directions, can solve problems such as lack of immune isolation performance, inability to isolate IgG, etc., to ensure the needs of cell growth and metabolism, and to be conducive to active preservation and reaction conditions. mild effect

Inactive Publication Date: 2012-09-12
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, according to existing literature reports, the ACA microcapsules made by sodium alginate and chitosan only through complex coacervation cannot isolate IgG, that is, they do not have immune isolation properties [Biomaterials.1999, 20 (8): 773- 83]

Method used

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  • Preparation method of microcapsule with function of immune isolation
  • Preparation method of microcapsule with function of immune isolation
  • Preparation method of microcapsule with function of immune isolation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] 1. According to the method of reference [Chinese Journal of Organ Transplantation, 2003, 24 (2):: 86-88], sodium alginate-chitosan microcapsules embedded with islet cells were prepared, and the microcapsules were immersed in a concentration of 0.05% (w / v) sodium alginate solution, reacted for 20 minutes.

[0023] 2. After washing with physiological saline, immerse the microcapsules in step 1 in a chitosan solution with a concentration of 0.05% (w / v), a molecular weight of 100,000, a deacetylation degree of 95%, and a pH of 6.0, and react for 20 minutes.

[0024] 3. After washing with physiological saline, immerse in a sodium alginate solution with a concentration of 0.05% (w / v), and react for 20 minutes.

[0025] 4. Wash with normal saline and store in normal saline ( figure 1 ).

[0026] 5. Take about 300-500 microcapsules on a glass slide, blot the surface moisture, and add 0.5mL dropwise to a concentration of 2.0mg·mL -1 FITC fluorescently labeled protein solution...

Embodiment 2

[0028] 1. According to the method of reference [Chinese Journal of Organ Transplantation, 2003, 24 (2):: 86-88], sodium alginate-chitosan microcapsules embedded with hepatocyte HepG2 were prepared, and the microcapsules were immersed in a concentration of 0.1 % (w / v) sodium alginate solution, reacted for 10 minutes.

[0029]2. After washing with physiological saline, immerse the microcapsules in step 1 in a chitosan solution with a concentration of 0.2% (w / v), a molecular weight of 60,000, a deacetylation degree of 95%, and a pH of 6.5, and react for 10 minutes.

[0030] 3. After washing with physiological saline, immerse in a sodium alginate solution with a concentration of 0.1% (w / v), and react for 10 minutes.

[0031] 4. Wash with normal saline and store in normal saline.

[0032] 5. Operate according to step 5 of Example 1 to investigate the immune isolation performance of the microcapsules. The results show that the fluorescence intensity of FITC-IgG in the capsule is ze...

Embodiment 3

[0034] 1. According to the method of reference [Chinese Journal of Organ Transplantation, 2003, 24 (2):: 86-88], sodium alginate-chitosan microcapsules embedded with embryonic stem cells were prepared, and the microcapsules were immersed in a concentration of 0.1% (w / v) sodium alginate solution, reacted for 10 minutes.

[0035] 2. After washing with physiological saline, immerse the microcapsules in step 1 in a chitosan solution with a concentration of 0.2% (w / v), a molecular weight of 20,000, a degree of deacetylation of 98%, and a pH of 6.8, and react for 5 minutes.

[0036] 3. After washing with physiological saline, immerse in a sodium alginate solution with a concentration of 0.1% (w / v), and react for 10 minutes.

[0037] 4. Repeat step 2 after washing with normal saline.

[0038] 5. Repeat step 3 after washing with normal saline.

[0039] 6. Wash with normal saline and store in normal saline.

[0040] 7. According to example 1 step 5, investigate the immune isolation ...

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Abstract

The invention relates to a preparation method of a microcapsule with a function of immune isolation. The method comprises a step of self-assembly through respective layer alternation of a calcium alginate-chitosan microcapsule prepared through complex coaceration and sodium alginate solution as well as chitosan solution. The aperture of the microcapsule is controlled accurately, so that the microcapsule can pass through bovine serum albumin (BSA), but can not pass through immunoglobulin IgG. Therefore, the calcium alginate-chitosan microcapsule with the function of immune isolation can be obtained.

Description

technical field [0001] The invention relates to the preparation of microcapsules, in particular to a preparation method of microcapsules with immune isolation performance. Background technique [0002] Cell transplantation is the only effective measure to treat diseases with complete loss of organ function. However, the serious lack of donor sources and immune rejection limit the development of cell transplantation technology. One of the effective ways to solve the problem of immune rejection is to use microcapsules as an immune isolation device, and use microencapsulation technology to encapsulate allogeneic living tissues or cells to isolate the graft from the host. The immune isolation performance of microcapsules is a necessary condition for microcapsules to be used in cell transplantation. Ideal microcapsules for cell transplantation must allow small molecular weight substances outside the capsule (nutrients required for cell survival such as serum albumin, growth fac...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01J13/02
Inventor 马小军谢红国于炜婷
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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