Method for screening high immunoglobulinlg IgG synthesizing ability lactation cow

A globulin, high-immunity technology, applied in the field of screening dairy cows, can solve the problems of difficult separation and purification, high production cost, low efficiency, etc., and achieve the effect of increasing the content of IgG and reducing the cost of feeding

Active Publication Date: 2008-09-24
INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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AI Technical Summary

Problems solved by technology

However, the high production cost of immune milk and the special requirements of processing technology have become the restrictive factors for the further development of immune milk; the structure-activity relationship and molecular mechanism of many bioactive peptides are still unclear
The low concentration of bioactive peptides, complex enzymatic hydrolysis conditions, and difficulty in s

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  • Method for screening high immunoglobulinlg IgG synthesizing ability lactation cow
  • Method for screening high immunoglobulinlg IgG synthesizing ability lactation cow

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Embodiment Construction

[0038] The inventor of the present invention randomly selects 299 dairy cows among about 1600 dairy cows in four cattle farms in Daxing District, Beijing, uses the method of the present invention to calculate the IgG index of dairy cows, and screens, and compares the IgG content in the milk of the screened dairy cows with that of the whole group of dairy cows IgG content in milk was compared.

[0039] Specific steps:

[0040](1) Collect milk samples from dairy cows, and record the milk production, lactation days and parity of individual dairy cows at the collection time point:

[0041] 299 healthy lactating Chinese Holstein dairy cows were randomly selected from Cangdafu, Innovation, Anding and Dongshengyuan four cattle farms in Daxing District, Beijing, and mastitis detection was carried out with recessive mastitis diagnostic solution to eliminate the cows with recessive mastitis. Mastitis diagnostic solution (DeLaval Company). All the test cows adopt the general test manag...

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Abstract

The invention discloses a method for screening lactating dairy cows with high immunoglobulin IgG synthesis capacity, the specific steps are as follows: (1) a milk sample of the dairy cow is collected; (2) the somatic cell number SCC, the milk fat rate, the milk protein, the lactose and the total solids of the collected milk sample are measured; (3) the somatic cell score SCS is obtained by utilizing the somatic cell number SCC for calculation; (4) the IgG index is calculated; (5) the dairy cows are screened: the dairy cows with the IgG index which is more than -4.3 are selected. The experiment shows that the method can accurately screen the dairy cows with high IgG content in the milk from the dairy herd, managers can clearly know the dairy cows with high IgG yield and carry out the centralized feeding of the dairy cows; compared with the dairy herd which is not screened, the IgG content in the milk product obtained can be greatly improved by more than 19.5 percent, and the feeding cost can be greatly reduced. The application of the method of the invention can remarkably improve the IgG content in the milk and can provide the possibility for the production of the milk which is rich in high immunoglobulin.

Description

technical field [0001] The invention relates to a method for screening dairy cows, in particular to a method for screening lactating dairy cows with high immunoglobulin IgG synthesis ability. Background technique [0002] Immunoglobulins are the most important immune active proteins in colostrum and milk. The immunoglobulins in milk mainly include IgG, IgM, and IgA. Immune colostrum or immune milk contains specific antibodies that can prevent and treat intestinal diseases, and can prevent some human infectious diseases, such as Shigella flexneri, Escherichia coli, Clostridium stubborn (Clostridium difficile), Cryptosporidium parvum, Streptococcus mutans, and Helicobacter pylori. The specific Ig in milk can effectively inhibit the reproduction of bacteria, inhibit bacterial colonization, inhibit the binding of substrates, inhibit the secretion of toxins from bacteria, prevent the binding of toxins to human cell receptors, neutralize toxins, and reduce bacterial activity. a...

Claims

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Application Information

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IPC IPC(8): G01N33/04G01N33/48G06F19/00
Inventor 王加启卜登攀刘光磊魏宏阳周凌云张春刚刘蕾
Owner INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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