Silicon-based rhodamine fluorescent staining reagent and its preparation method and application

A silicon-based rhodamine and fluorescent dyeing technology, used in the fields of immunology, organic chemistry and biochemistry, can solve the problems of small Stokes shift of antibody-labeled dyes and difficult to apply, and achieve small molecular weight, high yield, and detection sensitivity. high effect
CN112251043BActive Publication Date: 2022-03-01SICHUAN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
SICHUAN UNIV
Publication Date
2022-03-01

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Abstract

The invention discloses a silicon-based rhodamine fluorescent dyeing reagent as well as its preparation method and application, belonging to the fields of organic chemistry and biochemistry. The present invention uses the rhodamine derivatives substituted with silicon atoms as the basic skeleton, and synthesizes a silicon-based rhodamine fluorescent substance with a large Stokes shift and high fluorescence intensity capable of labeling immunoglobulin IgG by modifying different forms of aromatic amines. The staining reagent can be used for in vitro SARS-CoV-2 virus-specific antibody fluorescent ELISA detection. The silicon-based rhodamine fluorescent staining reagent of the present invention has a large Stokes shift (>140nm), can effectively avoid the mutual interference of excitation and emission light, and has high detection sensitivity, and the fluorescent ELISA detection method established based on the fluorescent antibody can be applied Microplate readers with different bandwidths have a wide range of applications.
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Description

technical field

[0001] The invention relates to the fields of organic chemistry and biochemistry, in particular to the technical field of immunology, and in particular to a silicon-based rhodamine fluorescent staining reagent and its preparation method and application. Background technique

[0002] SARS-CoV-2 virus is a novel coronavirus pathogen that caused 2019 Severe Acute Respiratory Syndrome (SARS). The main clinical manifestations of infected patients are respiratory symptoms and pneumonia. In February 2020, the Drug International Virus Taxonomy Committee officially named the virus SARS-CoV-2, and the World Health Organization (WHO) named the disease 2019 coronavirus disease (2019corona virusdisease, COVID-19). The SARS-CoV-2 virus has strong transmission ability, long incubation time, and high incidence rate. Traditional virus defense measures are not effective in controlling the virus. The global pandemic trend is obvious, and the situation of virus prevention and co...

Claims

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