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Goat pox indirect hemagglutination diagnosis reagent as well as preparation method and detecting method thereof

A diagnostic reagent and goat pox technology, applied in the field of goat pox forward indirect hemagglutination diagnostic reagent and its preparation, can solve the problems of low sensitivity and slow detection speed, and achieve the effect of good specificity and simple detection

Inactive Publication Date: 2012-01-11
GUIZHOU UNIV
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Problems solved by technology

[0003] The technical problem to be solved by the present invention is to overcome the defects of low sensitivity and slow detection speed of the existing methods for detecting goat pox disease, and provide a diagnostic reagent for goat pox positive indirect hemagglutination and a preparation method thereof. , rapid, accurate and simple detection of goat pox virus antibody

Method used

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Embodiment Construction

[0013] Goat pox reverse indirect hemagglutination diagnostic reagent is prepared according to the following ratio and method

[0014] 1. Preparation of aldylated red blood cells

[0015] Aseptically collect venous blood from healthy sheep, add 10 times the volume of PBS, centrifuge at 2000 rpm for 15 minutes, wash 5 times with PBS, and prepare 2.5% (volume) red blood cell suspension with phosphate buffer (0.1M, pH 7.4) , and then mixed the erythrocyte suspension with 1% (volume) glutaraldehyde aqueous solution at a volume ratio of 9:1, stirred slowly at room temperature for 1 hour, centrifuged at 2000 rpm for 15 minutes, and washed 5 times with phosphate buffer solution to obtain Aldehydization precipitates red blood cells.

[0016] 2. Sensitization: Dissolve the formaldehyde-precipitated erythrocytes in 10 times the volume of phosphate buffer (0.1M, pH 7.4), mix with an equal volume of purified goat pox virus antigen, stir at room temperature for 2 hours, and centrifuge at 2...

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Abstract

The invention discloses a goat pox indirect hemagglutination diagnosis reagent as well as a preparation method and a detecting method thereof. The hemagglutination diagnosis reagent comprises the following components by weight percent: 0.6-1.2% of goat pox virus antigen sensitized red blood cells, and 20-200 mu g / ml of purified goat pox virus antigen. Compared with the commonly used agar diffusion experiment, the goat pox indirect hemagglutination diagnosis reagent is prepared from purified goat pox virus antigen sensitized hydroformylated sheep red blood cells in the invention, thus, the goat pox virus antibody can be detected fast, accurately and simply, the result can be determined in 50 minutes, and the antibody with low valence can be detected; moreover, the goat pox indirect hemagglutination diagnosis reagent has good specificity, sensitivity and repeatability, and can be widely used for fast and substantive detection of goat pox virus antibodies in clinical vet, and can provide the basis for prevention, diagnosis and prevention and control of a disease.

Description

technical field [0001] The invention relates to the technical field of positive hemagglutination diagnostic reagents, in particular to a goat pox forward indirect hemagglutination diagnostic reagent and a preparation method and a detection method thereof. Background technique [0002] Goat pox is an endemic highly contagious infectious disease caused by goat poxvirus (GTPV), which can occur all year round and is common in spring and autumn, regardless of gender, age, or species. The World Organization for Animal Health (OIE) listed goat pox as a major infectious disease of category A, and in 1999, the Ministry of Agriculture of the People's Republic of China announced goat pox as a national first-class animal disease. In recent years, more than 20 provinces and cities in my country have successively reported the occurrence of goat pox epidemic, which has caused great economic losses and posed a serious threat to the booming goat breeding industry in my country. The traditio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569G01N33/531
Inventor 刘忠伟周碧君程振涛文明王开功嵇辛勤岳筠汪德生
Owner GUIZHOU UNIV
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