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93 results about "Duck hepatitis virus" patented technology
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Duck hepatitis is caused by the Avihepatovirus DHV-1 and DHV-3. It is a fatal disease of ducklings causing opisthotonus and hepatitis. DHV-1 is found worldwide and causes disease in young ducklings, usually <6 weeks of age and spreads rapidly within a flock. It is the most virulent of the DHV species. DHV-3 has only been reported in the USA.
GeXP (Gene Expression Profiler) detection kit for differentiating 11 kinds of duck viral diseases
ActiveCN103773899AStrong specificityImprove throughputMicrobiological testing/measurementDNA/RNA fragmentationDiseaseDuck hepatitis A virus
Owner:GUANGXI VETERINARY RES INST
Korean novel duck hepatitis viral antibody ELISA (Enzyme-Linked Immunosorbent Assay) detection kit
InactiveCN102127531ASolve the difficulty of purificationImprove securityRecombinant DNA-technologyFermentationDuck hepatitis A virusViral antibody
The invention discloses a Korean novel duck hepatitis viral antibody ELISA (Enzyme-Linked Immunosorbent Assay) detection kit. The detection kit contains an ELISA board coated by Korean novel duck hepatitis VP1 (Phenotypic Variance1) recombination protein, a sample diluent, concentrated washing liquid, an enzyme conjugate working solution, a chromogenic reagent (A), a chromogenic reagent (B), a stopping solution, a positive contrast solution and a negative contrast solution. The VP1 recombination protein is obtained by using the following method: using Korean novel duck hepatitis viruses as a material, augmenting and cloning the VP1 gene through an RT-PCR (Reverse Transcriptase-Polymerase Chain Reaction) method to obtain recombinant expression plasmid pMD (physical medium dependent)-VP1; then, directionally inserting to an expression vector pET-32a (+) and screening to obtain recombinant expression plasmid pET-32a(+)-VP1; and inducing, expressing and purifying by ITPG (Isopropyl beta-D-Thiogalactopyranoside) to obtain VP1 recombination protein. The detection kit is used for detecting the Korean novel duck hepatitis and has strong specificity, high sensitivity, simplicity of operation, easiness of popularization and application in a large-area range and wide market prospects.
Owner:POULTRY INST SHANDONG ACADEMY OF AGRI SCI
Preparation method of I-type duck hepatitis refined yolk antibodies
InactiveCN105367654AReduce contentReduce the risk of adverse reactionsEgg immunoglobulinsImmunoglobulins against virusesDuck hepatitis A virusYolk
The invention provides a preparation method of I-type duck hepatitis refined yolk antibodies. The preparation method comprises following steps: I-type duck hepatitis virus hyper-immune egg yolk is collected, is subjected to primary inactivation, is subjected to primary acidification extraction with an acetate buffer solution, and then is subjected to secondary inactivation taking octanoic acid as the inactivator and extraction agent; filtering clarification, filtering sterilization, and ultrafiltration concentration are carried out; and then formaldehyde is used for a third time of inactivation so as to obtain a yolk antibody solution. According to the preparation method, content of lipid in the finished product is reduced effectively via acidification water-octanoic acid method, occurrence rate of adverse reaction is reduced, the step of ultrafiltration concentration is beneficial for ensuring product antibody concentration, and increasing protection rate, in application, dilution ratio of the finished product is relatively high because of the high antibody concentration, so that impurity content is reduced indirectly, and safety is improved.
Owner:TIANJIN RINGPU BIO TECH
Molecular biology identification method of i-form duck hepatitis virus
InactiveCN101358247ARapid diagnosisIncreased sensitivityMicrobiological testing/measurementFluorescence/phosphorescenceDuck hepatitis A virusTissue sample
The invention discloses a molecular biological identification method for I type duck hepatitis virus, which comprises the steps of treating samples to be tested, preparing RNA of the samples, obtaining cDNA of the samples by reverse transcription, identifying I type duck hepatitis virus by the fluorescent quantitive RT-PCR method or the nested PCR method, etc. The molecular biological identification method has the advantages of fast diagnosis, high sensitivity and wide applied range, can be used for identifying DHV-1 from the tissue samples, cotton swabs, ducks or chicken embryo allantoic liquid or cell cultures.
Owner:SOUTH CHINA AGRI UNIV
Duck hepatitis virus type I LAMP (loop-mediated isothermal amplification) detection kit
ActiveCN102344973AHigh sensitivityEasy to operateMicrobiological testing/measurementMicroorganism based processesDuck hepatitis A virusLoop-mediated isothermal amplification
The invention discloses a duck hepatitis virus type I LAMP (loop-mediated isothermal amplification) detection kit. A LAMP technology is adopted; and two specific inner primers, two specific outer primers and two specific ring primers are designed according to a gene conserved region shared by duck hepatitis viruses type I. Due to the application of the duck hepatitis virus type I LAMP detection kit and a detection method established by the invention, the defects of long detection time, large workload, cross pollution, complex operation and the like in the prior art are overcome. The duck hepatitis virus type I LAMP detection kit has the strong specificity and high sensitivity, is rapid, has low cost and simpler operation method and is particularly suitable for the requirement on field rapid detection of the duck hepatitis virus type I in the primary-level areas.
Owner:GUANGXI VETERINARY RES INST
PCR-RFLP primer for distinguishing DHV-1 and new serotype and method
InactiveCN105132589AStrong specificityHigh sensitivityMicrobiological testing/measurementDNA/RNA fragmentationRestriction Enzyme Cut SiteRNA extraction
The invention discloses a PCR-RFLP primer for distinguishing DHV-1 and a new serotype and a method for distinguishing the DHV-1 and the new serotype. The primer is an upstream primer P1:5'-CAATTGAGGACATGGCTAAGAAA-3' and a downstream primer P2:5'-TCAGAGASCCRTCRAAWCCAGAAA-3. According to the method, distinguishing is conducted according to the DHV-1 and the restriction enzyme cutting site difference contained in a new serotype coding area and includes the steps that RNA extraction is conducted, and after corresponding target fragments are obtained through RT-PCR amplification, RFLP analysis is conducted after XhoI restriction enzyme cutting. The distinguishing method is simple and fast in operation and high in accuracy.
Owner:INST OF ANIMAL HUSBANDRY & VETERINARY FUJIAN ACADEMY OF AGRI SCI
GeXP Detection Kits for Identification of 11 Kinds of Duck Virus Diseases
ActiveUS20160060716A1Strong specificityNucleotide librariesMicrobiological testing/measurementDuck hepatitis A virusDisease
Provided herein is a GeXP detection kit for identification of 11 kinds of duck virus diseases. The detection kit includes a primer set for identifying or auxiliarily identifying pathogens of duck communicable diseases, including one or more of primer pair A, primer pair B primer pair C, primer pair D, primer pair E, primer pair F, primer pair G, primer pair H, primer pair I, primer pair J, primer pair K and primer pair L. The can kit detect, simultaneously avian influenza virus, subtype H5, H7 and H9 of avian influenza virus, duck hepatitis virus, duck enteritis virus, duck Tembusu virus, Newcastle disease virus, egg drop syndrome virus, Muscovy duck reovirus, Muscovy duck parvovirus and duck circovirus with the primer set, PCR reagent or primer pairs provided in the present invention.
Owner:GUANGXI VETERINARY RES INST
Triple polymerase chain reaction (PCR) kit for duck hepatitis virus type I, duck circoviruses and Muscovy duckling parvovirosis and application of triple PCR kit
ActiveCN102719564AStrong specificityHigh sensitivityMicrobiological testing/measurementMicroorganism based processesDuck hepatitis A virusSingle strand
The invention discloses a primer pair group and a kit for performing identification or auxiliary identification on duck-related viruses and application of the primer pair group and the kit. The primer pair group consists of three primer pairs, and the three primer pairs consist of two single-stranded deoxyribonucleic acids (DNA) which are shown as a sequence 1 and a sequence 2, a sequence 3 and asequence 4, and a sequence 5 and a sequence 6 respectively; and the duck-related viruses are at least one of Muscovy duckling parvovirosis, duck circoviruses and duck hepatitis virus type I. A triplepolymerase chain reaction (PCR) technology that three kinds of pathogens, namely the Muscovy duckling parvovirosis, the duck circoviruses and the duck hepatitis virus type I can be simultaneously detected and identified through one-time PCR is established, and has the advantages of high specificity and sensitivity (the lowest detection line is 1pg), low cost, high efficiency and the like; and moreover, an amplification result is directly determined by utilizing the difference of the length of amplified fragments in the aspect of primer design, so that the method is relatively simple, convenient, intuitive and practical during result determination.
Owner:GUANGXI VETERINARY RES INST
Indirect ELISA method and kit for detecting serum 3-type duck hepatitis virus a antibody
The invention discloses an indirect ELISA method and kit for detecting serum 3-type duck hepatitis virus a antibody and belongs to the technical field of serum antibody detection. The indirect ELISA method includes that serum 3-type duck hepatitis virus VP1 protein is utilized as an envelope antigen with the peridium quantity as 0.1-1mug per hole, HRP-mice anti-duck IgY is utilized as the HRP with the use concentration as 0.2-2mug / mL, and the coloration time is 10 minutes. The kit comprises a VP1 protein antigen envelope board, the HRP-mice anti-duck IgY, sample diluent, a scrubbing solution, TMB solutions A and B and a stop solution. The method and the kit can be used for detecting the serum 3-type duck hepatitis virus a antibody in duck serum and duck egg yolk, and the serum does not have cross reaction with positive serum of other viruses. Compared with a traditional neutral reaction detection method, the method has the advantage of being convenient and accurate.
Owner:WUHAN CHOPPER BIOLOGY
Muscovy duck hepatitis virus and method for preparing muscovy duck hepatitis virus refined egg yolk antibody by adopting same
ActiveCN105950564ARemission of symptoms of clinical onsetAvoid infectionEgg immunoglobulinsSsRNA viruses positive-senseAntigenWhite liver disease
The invention provides a muscovy duck hepatitis virus and a method for preparing a muscovy duck hepatitis virus refined egg yolk antibody by adopting the muscovy duck hepatitis virus. The muscovy duck hepatitis virus strain GS14 is preserved in the China Center for Type Culture Collection on April 27, 2016, and the accession number is CCTCC No: V201623. The method for preparing the muscovy duck hepatitis virus refined egg yolk antibody by adopting the muscovy duck hepatitis virus comprises the following steps: preparing antigens by adopting the muscovy duck hepatitis virus strain GS14; carrying out inactivation on the antigens; preparing antigens for vaccines; preparing inactivated vaccines; and preparing the muscovy duck hepatitis virus refined egg yolk antibody. The muscovy duck hepatitis virus and the preparation method have the beneficial effects that the prepared muscovy duck hepatitis virus refined egg yolk antibody can prevent and treat the white liver disease of the muscovy duck, so that an effective means is provided for preventing and treating the white liver disease of the muscovy duck clinically.
Owner:CHONGQING SANJIE ZHONGXIN BIOLOGICAL ENG CO LTD
Duck enteritis virus and the uses thereof
ActiveUS10202582B2Early onsetEfficient expressionAntibacterial agentsInactivation/attenuationFowlDuck enteritis virus
Owner:CEVA SANTE ANIMALE
Preparation of duck hepatitis I type virus indirect hemagglutination diagnostic antigen and kit
InactiveCN101423548AQuick responseEasy to useVirus peptidesMaterial analysisDuck hepatitis A virusType antigen
The invention relates to a method for preparing Duck hepatitis virus I type indirect blood coagulation diagnosing antigen, in particular to a method for preparing indirect blood coagulation diagnosing antigen by Duck hepatitis virus I type antigen sensibilization double hydroformylation mutton red cell. The Duck hepatitis virus I type indirect blood coagulation diagnosing antigen produced by the method has long storage time up to half a year; moreover, the diagnosing time is shortened and only half an hour is needed, and the use is convenient and simple; therefore, the method is suitable to be popularized and applied to the practical production.
Owner:陶海静
RT-PCR discriminating diagnosis primers for type 1 and new serotype duck hepatitis virus
InactiveCN106011315AIdentification method is simpleFast and Accurate Differential Diagnostic TestingMicrobiological testing/measurementMicroorganism based processesDuck hepatitis A virusNucleotide
The invention discloses a group of RT-PCR discriminating diagnosis primers for discriminating type 1 and new serotype duck hepatitis virus. A primer sequence is shown as a SEQ ID NO. 1-2. The primer is designed according to existed difference of a 3'-terminal nucleotide sequence of a type 1 and new serotype duck hepatitis virus whole-genome sequence, The group of the primers performs discriminating diagnosis in a specific amplification area for amplifying the type 1 and new serotype duck hepatitis virus according to the length difference of the nucleotide sequences, wherein size of a type 1 duck hepatitis virus amplification fragment is about 258 bp, and the size of a new serotype duck hepatitis virus amplification fragment is about 321 bp. The identification method of the primer has the advantages of easy and rapid operation, and high accuracy, only one group of the primers can discriminate the type 1 and new serotype duck hepatitis virus types, and the primers provide technical guarantee for aquatic bird healthy culture.
Owner:INST OF ANIMAL HUSBANDRY & VETERINARY FUJIAN ACADEMY OF AGRI SCI
Preparation methods for duck hepatitis virus immunogen and hyperimmune serum and application of duck hepatitis virus hyperimmune serum
InactiveCN104926939AThe preparation method requires low conditionsEasy to operateSerum immunoglobulinsImmunoglobulins against virusesDuck hepatitis A virusSerum ige
The invention provides preparation methods for duck hepatitis virus immunogen and hyperimmune serum and application of the duck hepatitis virus hyperimmune serum. According to the preparation methods and the application of the duck hepatitis virus hyperimmune serum, the duck hepatitis virus immunogen is obtained through inoculating a serum 1 type duck hepatitis virus CH60 strain DHAV-1 (Duck Hepatitis A Virus type 1) or a serum 3 type duck hepatitis virus CH1 strain DHAV-3 (Duck Hepatitis A Virus type 3) to an allantoic cavity of a chick embryo of 9-10 days old or a duck embryo of 10-12 days old and carrying out proliferation and treatment, and the hyperimmune serum is obtained through mixing the duck hepatitis virus immunogen with a Freund's complete adjuvant or Freund's incomplete adjuvant to prepare solutions of different concentrations, carrying out repeated immunization on immune animals and then sampling and collecting blood and can be applied to the diagnosis and detection on a duck hepatitis virus. The preparation methods provided by the invention have the advantages that the conditional requirements are low, the operation is simple, and the obtained immunogen can meet the requirements on the preparation of specific antiserum.
Owner:SICHUAN AGRI UNIV
Riemerella anatipestifer OmpA/MotB truncated recombinant protein, antibody and preparation method and application thereof
ActiveCN104974249AImmunoreactiveEasy to prepareAntibacterial agentsImmunoglobulins against animals/humansDuck hepatitis A virusSerum ige
The invention discloses a Riemerella anatipestifer OmpA / MotB truncated recombinant protein, its antibody and a preparation method and an application thereof. The recombinant protein has immunoreactivity similar to natural OmpA / MotB protein, can bind to RA antibody specifically, will not generate cross reactions with Salmonella anatum positive serum, duck colibacilosis positive serum, duck swollen head septicemia virus positive serum, avian influenza virus (H5) positive serum, duck plague virus positive serum, duck hepatitis virus positive serum and the like, and can be used as a detection antigen for detecting whole bacteria antibody. As the recombinant protein is not whole bacteria, there is no risk of spreading bacteria when the recombinant protein is applied to detection. In addition, the preparation method of the recombinant protein is simple, is suitable for large-scale industrial production, can realize standardization of products and is beneficial to result comparison between different laboratories.
Owner:SICHUAN AGRI UNIV
Dual-PCR (Polymerase Chain Reaction) assay kit for duck circovirus and duck hepatitis virus
ActiveCN102605104AStrong specificityThe result is easy to judgeMicrobiological testing/measurementDNA/RNA fragmentationDuck hepatitis A virusPcr assay
The invention discloses a dual-PCR assay kit for duck circovirus and duck hepatitis virus. The dual-PCR assay kit provides a primer group for assaying duck circovirus and duck hepatitis virus, which consists of a primer 1, a primer 2, a primer 3 and a primer 4; and the nucleotide sequences of the primer 1, the primer 2, the primer 3 and the primer 4 are sequence 1, sequence 2, sequence 3 and sequence 4 sequentially in a sequence table. The dual-PCR technique which can simultaneously assay and identify DuCV (duck circovirus) and DHV (duck hepatitis virus) as two pathogens by establishing PCR reaction just once has good specificity, moreover, the experiment utilizes the difference of amplified fragment lengths to directly determine an amplification result in the primer design, and thereby the method is simpler, more visual and more practical during result determination.
Owner:GUANGXI VETERINARY RES INST
Duck enteritis virus and the uses thereof
ActiveUS20190151439A1High speedEarly onsetViral antigen ingredientsAntiviralsDuck enteritis virusVirology
Owner:CEVA SANTE ANIMALE
Novel freeze-drying protective additive for duck virus hepatitis live vaccines
ActiveCN103417980AEasy to prepareSuitable for mass productionPowder deliveryAntiviralsDuck hepatitis A virusAntigen
The invention relates to a novel freeze-drying protective additive for duck virus hepatitis live vaccines. The novel freeze-drying protective additive has the advantages that the vaccine is low in loss of antigen activity during freeze drying, and the problem that the duck hepatitis virus is sensitive to the freeze drying is effectively solved; the duck virus hepatitis vaccines freeze-dried by the protective additive are good in heat resistance, and storage life thereof is longer than 24 months at the temperature of 2-8 DEG C, good activity is still maintained in ten days after a resistance aging test at the temperature of 37 DEG C, so that the problem of a strict low-temperature cold chain of the vaccines upon storage and transportation is solved, and meanwhile vaccine storage and transportation costs are greatly lowered.
Owner:兆丰华生物科技(南京)有限公司
Method for establishing goose embryo epithelial cell line and established goose embryo epithelial cell line
ActiveCN104152403AGood growth divisionHigh purityMicroorganism based processesViruses/bacteriophagesBiotechnologyDuck hepatitis A virus
The invention discloses a method for establishing goose embryo epithelial cell line and the established goose embryo epithelial cell line. The invention relates to the method for establishing the goose embryo epithelial cell line, which is characterized in that a primary goose tissue adherent method, a differential velocity enzymatic digestion and a monoclonal screening method are combined, so that primary culture condition can be optimized. The method has the advantages of simple operation process, and convenient popularization and application. The invention also relates to the goose embryo epithelial cell line established by the method, a preservation number of the goose embryo epithelial cell line is CCTCC NO: C2014137, The establishment of the goose embryo epithelial cell line solves the problem of no well-established goose source cell line in prior art. The invention also relates to a kit used for culturing and / or proliferating goose parvovirus, muscovy duck parvovirus and type I duck hepatitis virus and novel duck hepatitis virus, and is characterized in that a host cell to be infected is / or comprises the goose embryo epithelial cell line. The method for establishing the goose embryo epithelial cell line verifies the sensitive characteristic of the goose parvovirus, muscovy duck parvovirus and type I duck hepatitis virus and novel duck hepatitis virus.
Owner:SHANDONG BINZHOU ANIMAL SCI & VETERINARY MEDICINE ACADEMY
Gelatin particle-agglutination detection reagent kit suitable for 1type duck hepatitis virus antibody and its uses
InactiveCN101101297AStrong specificityHigh sensitivityMaterial analysisDuck hepatitis A virusAgglutination
The invention belongs to the bird immunology technology area, it concretely relates to a kit of acceptable for the one type duck hepatitis virus antibody emulsion agglutination and the application of testing one type duck hepatitis virus antibody emulsion agglutination. The kit of this invention contains box body and core reagent in the box body, positive check sample, negative check sample, sample cell, scale sample sucker and the carrier of observed result. The characteristic is that core reagent is latex antigen from sublimating e duck hepatitis virus AV2111 and inactivating one type duck hepatitis virus sensitization polystyrene latex, the positive check sample is one type duck hepatitis virus antiserum, the negative check sample is the green duck of a day old of no one type duck hepatitis mother source antibody. The carrier is the paper characteristic latex agglutination card of having hollowness. The invention also discloses the preparation method and application of this kit. The kit has the significance merit of high specificity, high sensitivity, handy operation, and quickly diagnose.
Owner:HUAZHONG AGRI UNIV
One tube PCR type kit for discriminating I type duck hepatitis virus and new I type duck hepatitis virus
InactiveCN103255230ARapid differential diagnosisSensitive differential diagnosisMicrobiological testing/measurementMicroorganism based processesDuck hepatitis A virusEmbryo
The invention discloses a one tube PCR type kit for discriminating duck hepatitis virus (DHV-1A) and new I type duck hepatitis virus (DHV-1C), and the kit comprises a primer, a reagent for treating sample to be tested, a PCR reagent and an instruction, wherein, the primer is SEQ ID Nos:1-3. The kit of the present invention is suitable for identifying DHV-1A virus and DHV-1C virus in liver, spleen and kidney tissue samples, cloaca cotton swab, duck or chicken embryo allantoic liquid and cell culture sample of duck.
Owner:HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
Duck enteritis virus and the uses thereof
ActiveUS20180187164A1Early onsetEfficient expressionAntibacterial agentsInactivation/attenuationDuck enteritis virusVirology
Owner:CEVA SANTE ANIMALE
Duck hepatitis virus (DHV) I type VP1 protein inhibitory peptide and application thereof
InactiveCN104193803APrevent proliferationDownregulation of viral copy numberPeptide/protein ingredientsAnimal feeding stuffAnimal virusDuck hepatitis A virus
The invention discloses a duck hepatitis virus (DHV) I type VP1 protein inhibitory peptide and application thereof, and belongs to the technical field of animal virus molecular biology. The inhibitory peptide is characterized in that DHV-1VP1 protein is obtained by means of a gene engineering method; and the DHV-1VP1 protein is used as a target, and a phage 12 peptide library is utilized for screening, so that the inhibitory peptide specifically bound with the DHV-1VP1 is obtained, and the amino acid sequence of the inhibitory peptide is LLADTTHHRPWT. Tests prove that the DHV I type VP1 protein inhibitory peptide is capable of inhibiting proliferation of DHV-1 in duck embryo fibroblasts, remarkably reducing the virus copy number of the DHV-1 in the duck embryo fibroblasts under different concentrations, and remarkably reducing the virus titer of the DHV-1 in the duck embryo fibroblasts. The DHV I type VP1 protein inhibitory peptide can be used for preparing anti-DHV medicines or feed additives, and has excellent application prospects in prevention and treatment of DHV-1.
Owner:HENAN UNIV OF SCI & TECH
GAOH derivative and medical application thereof
ActiveCN104530176AGood curative effectOrganic active ingredientsNervous disorderImmunologic disordersAutoimmune disease
The invention provides a GAOH derivative of a new structure. The compounds have great curative effects on inflammatory bowel diseases, hepatitis viruses, foot swelling, ear swelling, arthritis, pneumonia, nephritis, rhinitis, cerebral apoplexy, myocardial ischemia, atherosclerosis, arrhythmia, autoimmune disease, senile dementia, depression, schizophrenia, malignancy and tumor adjuvant therapy, virus infectious diseases, peptic ulcer, analgesia, antianaphylaxis, antiendotoxin, antishock and diabetes or diabetic complication.
Owner:WUHAN WORLDNER UNITED PHARMA
Type 3 duck hepatitis A virus mutation gene ISA-A117C-T1142A and construction method
ActiveCN110684781AAccelerate cultivationSpeed up the research processSsRNA viruses positive-senseViral antigen ingredientsHost tropismDisease
The invention discloses a type 3 duck hepatitis A virus mutation gene ISA-A117C-T1142A and a construction method. According to the mutant gene ISA-A117C-T1142A, 117 nucleotide of a type 3 duck hepatitis A virus virulent strain genome 5'UTR is mutated from A to C, and 1,142<nd> nucleotide is mutated from T to A, so that 164 amino acid of a virus VP0 protein is mutated from tyrosine of a parent strain to asparagine. The mutant gene virus strain has the same antigenicity as the parent strain, and has higher proliferation efficiency and virus titer on duck embryos than the parent strain, and can proliferate on chicken embryos with good immunogenicity and genetic stability. The pathogenicity of the mutant gene virus strain on ducklings is significantly reduced. The mutant gene virus strain can replicate in the ducklings but does not cause disease to the ducklings, and the important foundation is laid for a pathogenic mechanism of the virus and the development of vaccines. To sum up,the type 3 duck hepatitis A virus mutation gene ISA-A117C-T1142A virus strain is an ideal vaccine candidate strain, can be used for the study of the host tropism and attenuated mechanism of type 3 duck hepatitis virus, and has broad application prospects.
Owner:SICHUAN AGRI UNIV
Triple inactivated vaccine for duck circovirus disease, novel duck reovirus disease and duck virus hepatitis and preparation method of triple inactivated vaccine
PendingCN113491767AHigh titer contentAvoid adverse reactionsSsRNA viruses positive-senseViral antigen ingredientsDuck hepatitis A virusDisease
Owner:哈药集团生物疫苗有限公司
H9N2 subtype avian influenza virus-duck enteritis virus living-vector vaccine
InactiveCN103881982AShort cycleThere is no cross-species transferBacteriaMicroorganism based processesAnimal GeneticsAvian influenza virus
The invention belongs to the technical field of animals genetic engineering, in particular relates to an application of H9N2 subtype avian influenza virus-duck enteritis virus living-vector vaccine strain rDEV-HA9 in constructing a living-vector vaccine. The vaccine strain is preserved in the China Center for Type Culture Collection( the preservation number is CCTCC NO: V201403). The construction method of the living-vector vaccine disclosed by the invention comprises inserting H9N2 subtype avian influenza virus ha gene fragment in the duck enteritis virus artificial chromosome plasmid pBAC-C-KCE to obtain a recombinant plasmid pBAC-C-KCE-HA9; wherein the genetic structural compositions of the plasmid pBAC-C- KCE are shown in Figure 7 and the genetic structural compositions of plasmid pBAC-C-KCE-HA9 are shown in Figure 15.
Owner:HUAZHONG AGRI UNIV
One-step process PCR detection method for I-type duck hepatitis viruses and duck plague viruses
ActiveCN106399591AAvoid time costReduce labor costsMicrobiological testing/measurementDuck hepatitis A virusBiology
The invention provides a one-step process PCR detection method for duck hepatitis viruses and duck plague viruses. The method comprises the following steps: respectively selecting highly conservative genes in the duck hepatitis viruses and the duck plague viruses as a target fragment I and a target fragment II, and designing and synthesizing corresponding specific primers I and II; respectively extracting genome RNAs and DNAs of the duck hepatitis viruses and the duck plague viruses, mixing the genome RNAs and DNAs as templates, and carrying out double PCR amplification; and analyzing the obtained double PCR products by virtue of agarose gel electrophoresis. The one-step process PCR detection method provided by the invention has the advantages that the operation is simple, and the time and the labor are saved; PCR and RT-PCR can be carried out in the same reaction system, and DNA viruses (duck plague viruses) and RNA viruses (duck hepatitis viruses) can be simultaneously amplified and detected, so that the detection time is greatly shortened, and the detection human cost is greatly lowered; the specificity is good, highly specific segments can be amplified, and impurities in products are extremely few; and the one-step process PCR detection method is suitable for being generalized and used.
Owner:HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
Nano-antibody for specifically recognizing duck hepatitis A virus 1
ActiveCN109021099ASpecific recognition abilityStrong specificityDigestive systemBiological material analysisDuck hepatitis A virusSite-directed mutagenesis
The invention discloses a nano-antibody for specifically recognizing duck hepatitis A virus 1. The amino acid sequence of the nano-antibody is represented by SEQ ID NO.1. The nano-antibody has the advantages of small molecular weight, high stability, excellent antigen binding performance and easy expression, and provides a basis for the detection of the duck hepatitis A virus 1 and the developmentof treatment medicines. A mutant with good endophilicity, specificity and stability can be obtained through reconstructing by using a random or site-directed mutagenesis technology with the nano-antibody as a precursor, and is used for developing proteins or polypeptides for being further used in medicines, industries and agriculture.
Owner:SHANDONG AGRICULTURAL UNIVERSITY
Preparation method and use of soluble type-I duck hepatitis virus 3C protein
The invention relates to a preparation method and use of a soluble type-I duck hepatitis virus 3C protein. The preparation method comprises connecting nucleotides at 3th to 574th sites shown in the formula of SEQ ID NO. 3 to polyclonal cleavage sites of a pET-32(a)+vector, carrying out activation in an Amp-resistant LB culture medium based on escherichia coli BL21 as host bacteria, and carrying out induced expression under conditions of the IPTG final concentration of 0.2-1.4 mmol / L and a temperature of 16-37 DEG C for 2-12h to obtain a soluble type-I duck hepatitis virus 3C protein. The protein is obtained by soluble expression, is natural, is free of denaturant-based denaturation and renaturation and can be used for 3C protease activity detection and 3C protease inhibitor screening. Thepreparation method has a great importance for research, development and screening of a drug for resisting type-I duck hepatitis viruses.
Owner:SICHUAN AGRI UNIV
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