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Muscovy duck hepatitis virus and method for preparing muscovy duck hepatitis virus refined egg yolk antibody by adopting same

A duck hepatitis virus, hepatitis virus technology, applied in the direction of virus/bacteriophage, biochemical equipment and methods, viruses, etc., can solve the problems of low cure rate, prolonged slaughter time, and blocked development of Muscovy ducks.

Active Publication Date: 2016-09-21
CHONGQING SANJIE ZHONGXIN BIOLOGICAL ENG CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After clinical onset, conventional antiviral combined with antibacterial treatment plan, the effect is not good, the cure rate is less than 40%, the course of disease is long, the development of resistant Muscovy duck is hindered, the feed meat is more than 20% higher than that of normal Muscovy duck, and the time for slaughter is extended by 15 days Above, the development is irregular, which has seriously affected the economic benefits of Muscovy duck breeding

Method used

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  • Muscovy duck hepatitis virus and method for preparing muscovy duck hepatitis virus refined egg yolk antibody by adopting same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0079] (1) Preparation of antigen:

[0080] Dilute Muscovy duck hepatitis virus GS14 strain 10,000 times with normal saline, inoculate 12-day-old susceptible Muscovy duck embryos through the allantoic cavity, 0.2ml / embryo, collect and inoculate dead Muscovy duck embryo allantoic fluid within 60-168 hours, no If the bacteria pass the test, it is an antigen and stored at -40°C; at the same time, samples are taken for identification; the identification method is as follows:

[0081] 1.1 Virus content

[0082] The antigen was serially diluted 10 times with normal saline, and 10 -5 、10 -6 and 10 -7 Inoculate 12-day-old susceptible Muscovy duck embryos at 3 dilutions, inoculate 5 embryos for each dilution, 0.2ml / embryo, seal the hole with wax, and incubate at 37°C, discard Muscovy duck embryos that died before 24 hours Regardless, the dead muscovy duck embryos were collected for 24 to 168 hours, and the virus content was calculated by the Reed-Muench method to be 10 ～ 6.25 ELD...

Embodiment 2

[0134] (1) Preparation of antigen:

[0135] Dilute Muscovy duck hepatitis virus GS14 strain 5000 times with normal saline, inoculate 11-day-old susceptible Muscovy duck embryos through the allantoic cavity, 0.1ml / embryo, collect and inoculate dead Muscovy duck embryo allantoic fluid within 60-168 hours, no If the bacterium is qualified in the test, it is an antigen, and it is stored at -20°C; at the same time, a sample is taken for identification; the identification method and identification results are the same as in Example 1;

[0136] (2) Antigen inactivation:

[0137] The antigen was added to a formaldehyde solution with a final volume concentration of 0.1%, and inactivated at 37°C for 24 hours, during which the antigen was shaken 4 times for 3 minutes each time; the formaldehyde content in the formaldehyde solution was 36%;

[0138] (3) Antigens for preparing vaccines:

[0139] Centrifuge the inactivated antigen at 8°C at 4,500 rpm for 20 minutes, collect the supernatan...

Embodiment 3

[0168] (1) Preparation of antigen:

[0169] Dilute Muscovy duck hepatitis virus GS14 strain 8000 times with normal saline, inoculate 12-day-old susceptible Muscovy duck embryos through the allantoic cavity, 0.15ml / embryo, collect and inoculate dead Muscovy duck embryo allantoic fluid within 60-168 hours, no If the bacterium is qualified in the test, it is an antigen, and it is stored at -30°C; at the same time, a sample is taken for identification; the identification method and identification results are the same as in Example 1;

[0170] (2) Antigen inactivation:

[0171] The antigen was added to a formaldehyde solution with a final volume concentration of 0.1%, and inactivated at 37°C for 24 hours, during which the antigen was shaken 4 times for 3 minutes each time; the formaldehyde content in the formaldehyde solution was 38%;

[0172] (3) Antigens for preparing vaccines:

[0173] The inactivated antigen was centrifuged at 4200 rpm for 25 minutes at 6°C, and the supernata...

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Abstract

The invention provides a muscovy duck hepatitis virus and a method for preparing a muscovy duck hepatitis virus refined egg yolk antibody by adopting the muscovy duck hepatitis virus. The muscovy duck hepatitis virus strain GS14 is preserved in the China Center for Type Culture Collection on April 27, 2016, and the accession number is CCTCC No: V201623. The method for preparing the muscovy duck hepatitis virus refined egg yolk antibody by adopting the muscovy duck hepatitis virus comprises the following steps: preparing antigens by adopting the muscovy duck hepatitis virus strain GS14; carrying out inactivation on the antigens; preparing antigens for vaccines; preparing inactivated vaccines; and preparing the muscovy duck hepatitis virus refined egg yolk antibody. The muscovy duck hepatitis virus and the preparation method have the beneficial effects that the prepared muscovy duck hepatitis virus refined egg yolk antibody can prevent and treat the white liver disease of the muscovy duck, so that an effective means is provided for preventing and treating the white liver disease of the muscovy duck clinically.

Description

technical field [0001] The invention relates to a muscovy duck hepatitis virus and a method for preparing the refined yolk antibody of the muscovy duck hepatitis virus by using the virus. Background technique [0002] Since 2013, Muscovy ducks have been prevalent in some areas of my country. The clinical symptoms are a sharp increase in feed intake and then a basic hunger strike. They are depressed, like to lie down, have yellow-green loose stools, and occasionally have respiratory rales. The sick duck is dehydrated and emaciated. Before death, there were neurological symptoms similar to duck viral hepatitis; autopsy symptoms were occasional pericardial effusion, myocardial hemorrhage and pancreatic necrosis, common liver enlargement, at least 20% larger than the normal Muscovy duck liver of the same age, and the background color of the whole liver It is dark yellow or off-white instead of the normal dark red of the liver. There are dense hemorrhage spots in the liver and pale...

Claims

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Application Information

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IPC IPC(8): C12N7/00C07K16/10C07K16/02
CPCC07K16/1009C07K2317/11C12N7/00C12N2770/32421
Inventor 魏思远赵光伟
Owner CHONGQING SANJIE ZHONGXIN BIOLOGICAL ENG CO LTD
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