Duck hepatitis virus type I LAMP (loop-mediated isothermal amplification) detection kit

Abstract

The invention discloses a duck hepatitis virus type I LAMP (loop-mediated isothermal amplification) detection kit. A LAMP technology is adopted; and two specific inner primers, two specific outer primers and two specific ring primers are designed according to a gene conserved region shared by duck hepatitis viruses type I. Due to the application of the duck hepatitis virus type I LAMP detection kit and a detection method established by the invention, the defects of long detection time, large workload, cross pollution, complex operation and the like in the prior art are overcome. The duck hepatitis virus type I LAMP detection kit has the strong specificity and high sensitivity, is rapid, has low cost and simpler operation method and is particularly suitable for the requirement on field rapid detection of the duck hepatitis virus type I in the primary-level areas.

Description

technical field [0001] The invention relates to the field of duck type I hepatitis virus detection, in particular to a duck type I hepatitis virus LAMP detection kit. Background technique [0002] Duck Hepatitis Virus type I is the causative agent of a highly lethal viral infectious disease targeting ducklings. The disease mainly affects ducklings within 4 weeks of age, and the mortality rate is as high as more than 90%. It is one of the most serious infectious diseases that endanger the duck industry. [0003] The traditional detection methods of duck hepatitis I virus include serum neutralization test, agar diffusion test and ELISA, etc., but these tests have disadvantages such as time-consuming, low sensitivity, and difficult standardization, which have certain limitations in practical application. At present, although duck hepatitis I virus PCR detection methods and fluorescent quantitative PCR detection methods have been established, their detection sensitivity is rela...

AI Technical Summary

Problems solved by technology

[0003] The traditional detection methods of duck hepatitis I virus include serum neutralization test, agar diffusion test and ELISA, etc., but these tests have disadvantages such as time-consuming, low sensitivity, and difficult standardization, which have certain limitations in practical application

At present, although duck hepatitis I virus PCR detection methods and fluorescent quantitative PCR detection methods have been established, their detection sensitivity is relatively high, but conventional PCR requires the use of a PCR machine and gel electrophoresis, and fluorescent quantitative PCR requires the use of more expensive Fluorescent quantitative PCR instruments and reagents, etc., so it is difficult to meet the needs of grassroots on-site detection

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