78results about How to "Avoiding the False Positive Problem" patented technology

A gene chip for detecting viruses of leguminous plant is disclosed, several probes and nature-control collations are fixed on surface of solidoid carrier, the probes has the nucleotide sequence showed on from SEQ ID No.1 to SEQ ID No.80; the nature-control collations consists of the surface chemistry LUT, fluorescence labeling LUT, positive LUT, negative LUT, cross LUT and blank control. The advantages and innovations of the invention are following: practicability, quick-speed, and idiocrasy, and sensitivity, low activity cost, repeatability, stable and reliable results. The testing system can guarantee the repeatability and accuracy of results that is proofed by the repeatability and stability test, the system can be widely used in area of plant quarantine of the outbound and enter the country test and quarantine system, the safety supervision of plant source food, the diagnosis of plant virus in plant protecting station and the authenticate of pathogenesis in research of plant taxonomic virology. The gene chip testing call for detecting viruses of leguminous plant can be used in about 600 daily quarantine pursuits of leguminous plant source food.

The invention discloses a process for detecting DNA, RNA and ultramicro protein, which can easily remove uncombined probe and other foreign matter through firstly sandwiching the ELISA detecting target molecular in liquid phase with capture probe 1 (can be ribonucleotide or antibody) on bead capture probe and capture probe 2 (can be ribonucleotide or antibody) on nano combining probe, and then absorbing magnetic beads with magnet. The detecting target molecular is amplified thousands of times since nano probe combining probe is combined with thousands of bar code DNA which is marked with biotin, each molecular bar code DNA can combine with 3 molecular DNA sequence which comprises T7 RNA promoter through utilizing a streptavidin-biotin system, thereby quantitatively detecting bar code DNA with the TMA technology, wherein bar code DNA is in proportion to target molecular content. The TMA technology can transcribe out of 10 thousand molecular DNA for special DNA sequence which comprises T7 RNA promoter in 3 hours according to a molecular DNA template, and can relatively amplify 10 thousand times bar code DNA.

The invention relates to a free DNA library construction method. A 3' end of a single-stranded free DNA is connected with a sole sequence tag; the sole sequence tag comprises a random sequence and a linker sequence; an upstream primer and a downstream primer for PCR amplification are added for PCR amplification; the upstream primer is a primer designed aiming at a target low frequency mutation; the downstream primer is the primer corresponding to the linker sequence. In addition, the invention further relates to a detection method for low and medium frequency mutation in a free DNA. The 3' end of the single-stranded free DNA is connected with the sole sequence tag; the upstream primer and the downstream primer for PCR amplification are added for PCR amplification; one end of each of the upstream primer and the downstream primer is connected with the sequence tag respectively; the amplified DNA is sequenced online on an Illumina platform. According to the method, detection on cfDNA low frequency mutation can be simply and efficiently achieved; the target of fetal inheritance or tumor cell mutation detection can be achieved through cfDNA detection.

The invention discloses a duck hepatitis virus type I LAMP (loop-mediated isothermal amplification) detection kit. A LAMP technology is adopted; and two specific inner primers, two specific outer primers and two specific ring primers are designed according to a gene conserved region shared by duck hepatitis viruses type I. Due to the application of the duck hepatitis virus type I LAMP detection kit and a detection method established by the invention, the defects of long detection time, large workload, cross pollution, complex operation and the like in the prior art are overcome. The duck hepatitis virus type I LAMP detection kit has the strong specificity and high sensitivity, is rapid, has low cost and simpler operation method and is particularly suitable for the requirement on field rapid detection of the duck hepatitis virus type I in the primary-level areas.

The invention discloses a primer and probe sequence used for LAMP-LFD detection of vibrio fluvialis and application of the primer and probe sequence. The primer and probe sequence is characterized by comprising three pairs of primers of LAMP including VflompU-F3 and VflompU-B3, VflompU-FIP and VflompU-BIP and VflompU-LF and VflompU-LB and a probe VflompU-HP, and a nucleotide sequence is shown as SEQ NO1-NO7. The primers and the probe are utilized to realize visual detection of vibrio fluvialis through a step of amplification by an LAMP reaction system, a step of enabling the probe to be hybridized with an LAMP reaction product and a step of LFD detection. The primer and probe sequence has the advantages of higher quickness, specificity and sensitivity, simplicity in instrument need, conduciveness to early diagnosis and detection of vibrio fluvialis and capability of meeting detection needs of primary detection mechanisms and on-site epidemic focuses.

The invention relates to a gene chip for detecting food-home virus and a kit, belonging to the inspection field. The surface of a solid phase carrier is fixed with a plurality of detection probes and quality control contrast probes, wherein, the detection probes consist of the probes for detecting hepatitis A virus, human astrovirus, norwalk virus G1, norwalk virus G2, rotavirus, polio virus 1, polio virus 2 and polio virus 3; and the quality control contrast probes consist of a spotting positive quality control probe, a chip hybridization positive quality control probe and a chip negative quality control probe. The gene chip and the kit have the advantages of: (1) high throughout: five common viruses are integrated and detected simultaneously, and the practicability is strong; (2) rapidness: the detection time is only 4 hours; (3) specificity: the false positive caused by the cross reaction is avoided; (4) flexibility: the detection flexibility of the chip is 10<8> virus particles per gram of the tissue sample, which is higher than that of RT-PCR; and (5) good repetitiveness and stable results. The gene chip and the kit can be widely applied to the food safety inspection of the inspections system.

The invention discloses an LAMP assay kit for identifying virulent and avirulent strains of mycoplasma gallisepticum. A primer group A provided by the LAMP assay kit consists of a primer group a and a primer group b; the primer group a consists of a primer 1 and a primer 2; the primer group b consists of a primer 3 and a primer 4; and the nucleotide sequences of the primer 1, the primer 2, the primer 3 and the primer 4 are sequence 1, sequence 2, sequence 3 and sequence 4 sequentially in a sequence table. An experiment for the LAMP assay kit proves that the primers and the method only need anordinary water bath rather other an expensive PCR (Polymerase Chain Reaction) instrument but have higher sensitivity than PCR assay, moreover, a result does not need to be observed by way of gel electrophoresis, and the LAMP assay kit is easy and rapid to operate, and is particularly suitable for field assay in grass roots.

The invention discloses primers and a probe for edwardsiella tarda LAMP-LFD (Loop-Mediated Isothermal Amplification-Lateral Flow Dipstick) detection and application of the primers and the probe. The primers and the probe are characterized by comprising three pairs of primers, namely EtaompA-F3, EtaompA-B3; EtaompA-FIP, EtaompA-BIP; EtaompA-LF and EtaompA-LB, of LAMP, and a probe EtaompA-HP, wherein the nucleotide sequence is shown as SEQ NO1-NO7; the visible detection of edwardsiella tarda is realized by utilizing the primers and the probe, and an amplification step of an LAMP reaction system, a hybridization step of the probe and an LAMP reaction product, and an LFD detecting step; the primers and the probe have the advantages that the rapidness, specificity and sensitivity are higher, the instrument requirement is simple, the primers and the probe are favourable for the early diagnosis and detection of the edwardsiella tarda, and the requirements on the detection of a primary detecting mechanism and an on-site epidemic focus can be met.

The present invention discloses primers and a probe for streptococcus iniae LAMP-LFD visual detection, and an application of the primers and the probe. According to the present invention, three pairs of LAMP primers such as gyrB-F3, gyrB-B3, gyrB-FIP, gyrB-BIP, gyrB-LF and gyrB-LB, and a probe gyrB-HP are provided, and the nucleotide sequences are represented by SEQID NO.1-EQID NO.7; and with the primers and the probe, a LAMP reaction system amplification step, a LAMP reaction product probe hybridization step and a LFD detection step are performed so as to achieve streptococcus iniae visual detection and further be used for kit preparation, wherein advantages of high rapidness, high specificity and high sensitivity are provided, instrument requirements are simple, early diagnosis and detection of streptococcus iniae can be easily achieved, and requirements of grass-roots detection facilities and field epidemic focus detection.

The invention discloses a dynamic social outsourcing data keyword query and verification method based on a block chain, and the method comprises the steps that an OSN operator outsources social data to a data provider, and the data provider provides data for a data consumer; the OSN operator constructs an auxiliary information block chain network and issues auxiliary information to the auxiliary information block chain network, wherein the auxiliary information block chain network constructs a block chain by nodes in an auxiliary information block chain; the data consumer verifies the authenticity of the query data through the auxiliary information block chain. The invention provides an efficient self-balancing Merkle hash tree data structure. The dynamic change of social data is supported, an updating algorithm supporting a self-balancing Merkle hash tree data structure is provided, a data consumer is allowed to efficiently check the authenticity of a queried data result, the decentralization of stored auxiliary information is realized, and the safety of the stored auxiliary information is improved; according to the invention, the social data is allowed to change dynamically, andthe expandability of the system is improved.

The invention relates to nucleic acid detection equipment capable of continuously working. The nucleic acid detection equipment comprises a nucleic acid amplification reaction module, a fluorescence detection device and a residue removal module, wherein the nucleic acid amplification reaction module comprises a micro-fluidic chip, a to-be-detected sample mixed solution can be injected into a flow channel of the micro-fluidic chip and an amplification reaction chamber, and a nucleic acid amplification reaction is carried out in the amplification reaction chamber; the fluorescence detection device is suitable for detecting a fluorescence signal of the amplification reaction chamber so as to obtain the amount of an amplification product in the amplification reaction chamber in real time according to the fluorescence signal; and the residue removal module comprises a high-temperature fluid cleaning module, and the high-temperature fluid cleaning module is used for injecting fluid with the temperature of 200 DEG C or above into the amplification reaction chamber of the nucleic acid amplification reaction module and the flow channel communicating with the amplification reaction chamber when a detection result of the nucleic acid detection equipment is positive, so that positive residues are removed. According to the nucleic acid detection equipment capable of continuously working, the positive residues in the equipment can be automatically removed, so that false positive of subsequent samples is avoided; and meanwhile, the use cost can be remarkably reduced.

The invention discloses a preparation method and application of an adamantanamine simulated magnetic molecularly-imprinted material, belonging to the technical field of food safety detection and solving the problem of false positive caused by high template leakage tendency in the adamantanamine separation and enrichment process in the prior art. The preparation method comprises the following steps: by using urotropine as a template molecule of simulated adamantanamine, 2-methacrylic acid as a functional monomer, 2-methyl-glycol-1,2-acrylate as a crosslinking agent and 2,2'azodiisobutyronitrile as an initiator, uniformly dispersing and mixing, initiating polymerization, and drying, thereby synthesizing the adamantanamine simulated magnetic molecularly-imprinted polymer. The target substance adamantanamine can be quickly combined to the surface of the synthesized magnetic molecularly-imprinted polymer, and quickly separated from the reaction system under the action of an external magnetic field, thereby efficiently and specifically extracting and separating the adamantanamine from the complex matrix, and enhancing the sample pretreatment efficiency and recovery rate.

The invention discloses a cell nucleus center point detection method based on a multi-task convolutional neural network. The method comprises the following steps: dotting and marking central points ofcell nucleuses, generating two types of masks during training directly according to coordinates of dotting, with a single type of Gaussian kernel being a Gaussian distribution two-dimensional array with the central point coinciding with the marked central point, and two types of equivalent kernels being an equivalent circular structure element two-dimensional array with the central point coinciding with marking data; a full convolutional network structure is adopted, two types of equivalent structure masks are firstly used for training two types of segmentation models, parameters of the segmentation models are used for initializing a multi-task model, and then the whole model is finely adjusted by combining losses of two task branches; and during prediction, inputting an RGB image, outputting two types of segmentation images and a single-type probability image by the model, and determining the position and the type of a final cell nucleus by combining the two types of segmentation images. According to the method, the problems of false negative, false positive and low convergence rate during direct regression of Gaussian nucleuses or equivalent structural element segmentation during pathological image cell nucleus detection are solved.

The present invention provides a visual detection method for PCR (polymerase chain reaction) amplification and an endpoint. The method comprises the steps as follows: adding a visible light dye with aconcentration of 0.1-0.3 mM into an amplification system before the PCR amplification reaction, and judging the reaction result by observing the color change of a complex formed by the visible lightdye and substances in the reaction system. The detection phase visually determines the detection result. The method is simple and easy, avoids cross-contamination caused by the cover opening, avoids harm to the human body, and reduces the detection cost.

The invention provides a parathyroid gland composite detection device, and belongs to the technical field of biological tissue detection and identification. The parathyroid gland composite detection device is characterized by comprising a probe, wherein a blood oxygen detection device and a parathyroid gland autofluorescence detection device are arranged on the probe. The parathyroid gland composite detection device further comprises a Raman detection device, wherein the Raman detection device comprises a Raman incident optical fiber, a return optical fiber, a Raman laser and a Raman spectrometer; the Raman incident optical fiber is connected with the Raman laser; and the Raman return optical fiber is connected with a processor. Compared with the prior art, the parathyroid gland compositedetection device can accurately detect the position of a parathyroid gland and judge the blood supply of the parathyroid gland at the same time; and in addition, the parathyroid gland composite detection device can effectively distinguish fat from the parathyroid gland through Raman scattering which is found first.

The invention discloses a primer set for detecting duck hepatitis A virus type 3, a kit and applications, and belongs to the technical field of virus detection. Two specific internal primers and two specific external primers, the kit based on the detection primers and applications for non-diagnostic purposes are included. The primer set, the kit and the applications are good in specificity, high in sensitivity, rapid, low in cost, more convenient in operation method and very suitable for primary laboratories to detect the duck hepatitis A virus type 3; and therefore, the defects in the prior art of long detection time, complex operation and expensive instruments can be solved.

The invention belongs to the field of medical detection, and particularly relates to a C-reactive protein colloidal gold detection reagent card as well as a preparation method and application thereof.The colloidal gold detection reagent card provided by the invention has the characteristics of low cost, no need of treatment of a detection sample, short detection time, high accuracy and the like,and can achieve the purposes of avoiding conventional blood sampling and painless detection.