3results about How to "Avoiding the False Positive Problem" patented technology

The application provides a Septin9 methylation detection composition and application thereof, and the composition comprises a methylation-dependent restriction enzyme, a capture oligonucleotide, a universal primer and a Septin9 methylation-specific primer; the capture oligonucleotide comprises a first universal sequence, a folding sequence and a binding capture sequence from 5' end to 3' end in sequence; the folding sequence is at least partially identical to a 5' end sequence of a Septin9 methylation site after the methylation-dependent restriction enzyme is cut; and the binding capture sequence is specifically bound to a fragment region where the detected Septin9 methylation site is located. The application is based on a methylation-dependent restriction enzyme and a universal primer fluorescence quantitative PCR technology, does not need bisulfite conversion, and realizes accurate and specific Septin9 methylation detection.

The application belongs to the technical field of biological detection and antibody engineering, and particularly relates to a nanobody for detecting tomato brown rugose fruit virus and a preparation method and application thereof. The application discloses a specific nanobody for detecting tomato brown rugose fruit virus and application thereof. Complementarity determining region sequences of the nanobody are shown in SEQ ID NO: 4-6; and a complete variable region amino acid sequence of the nanobody is shown in SEQ ID NO: 1. The application simultaneously provides a nucleotide sequence for encoding the nanobody, a recombinant expression vector, a host cell and a preparation method thereof. The nanobody has high affinity and excellent thermal stability to ToBRFV coat protein, and has no cross reaction with TMV, ToMV and other related viruses. A detection kit and a colloidal gold test strip constructed based on the antibody have a detection limit of 10 pg / mL, and are suitable for rapid screening and field quantitative diagnosis of ToBRFV.

The invention provides a kit based on high-throughput sequencing of crop pathogens and an application method, and the kit comprises a 16S full-length universal primer aiming at the 16S full length of the crop pathogens, an ITS full-length universal primer aiming at the ITS full length of the crop pathogens, and a specific primer for specifically detecting 27 crop pathogens, 27 pathogen crops can be rapidly detected by adopting a nanopore sequencing method.