Dual-PCR (Polymerase Chain Reaction) assay kit for duck circovirus and duck hepatitis virus

A duck circovirus and duck hepatitis virus technology, applied in the biological field, can solve the problems of immunosuppression, damage to the immune system of sick ducks, economic losses in the duck farming industry, etc., and achieve good specificity, important practical significance, and important practical value. Effect
CN102605104AActive Publication Date: 2012-07-25GUANGXI VETERINARY RES INST
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
GUANGXI VETERINARY RES INST
Publication Date
2012-07-25

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Abstract

The invention discloses a dual-PCR assay kit for duck circovirus and duck hepatitis virus. The dual-PCR assay kit provides a primer group for assaying duck circovirus and duck hepatitis virus, which consists of a primer 1, a primer 2, a primer 3 and a primer 4; and the nucleotide sequences of the primer 1, the primer 2, the primer 3 and the primer 4 are sequence 1, sequence 2, sequence 3 and sequence 4 sequentially in a sequence table. The dual-PCR technique which can simultaneously assay and identify DuCV (duck circovirus) and DHV (duck hepatitis virus) as two pathogens by establishing PCR reaction just once has good specificity, moreover, the experiment utilizes the difference of amplified fragment lengths to directly determine an amplification result in the primer design, and thereby the method is simpler, more visual and more practical during result determination.
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Description

technical field

[0001] The invention relates to the field of biotechnology, in particular to a double PCR detection kit for duck circovirus and duck hepatitis virus. Background technique

[0002] Duck circovirus (Duck Circovirus, DuCV) and duck hepatitis virus (DHV) are both common in ducks. Duck circovirus (DuCV) can cause symptoms such as growth retardation, messy feathers, and weight loss in ducks, and can infect the immune system of poultry, causing Immunosuppressive. Duck viral hepatitis caused by duck hepatitis virus (DHV) is a rapidly spreading viral disease that can make sick ducks highly lethal. The disease course is short and death is fast, and the mortality rate can be as high as 100%. These two diseases are widely prevalent, spread rapidly, have high morbidity and mortality, and are easy to be mixed with infection. At present, they are more harmful to waterfowl worldwide and bring huge economic losses to the duck industry. Duck circovirus destroys the immune sy...

Claims

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