45results about How to "Speed up the research process" patented technology

The invention relates to the technology of the near space aerocraft. In order to provide a system-level consistency check novel model verification system output on the basis of the dynamic time sequence of the near space aerocraft model, give qualitative model verification based on the monte carlo method, carry out risk analysis to the model, comprehensively carry out consistency check to a model to be verified and give a visual verification criterion, the invention adopts the technical scheme that the verification and simulation realization method for a near space aerocraft model comprises the following steps of: (1) on the basis of the monte carlo simulation method, carrying out qualitative verification; (2) carrying out the qualitative model verification based on a time domain analysis method; and (3) carrying out qualitative model verification based on risk analysis, taking a model parameter as a risk to give a visual qualitative model verification criterion, giving a model comparison standard when a plurality of models to be verified exist, verifying whether the model can be safely used, and giving a safe-use range. The verification and simulation realization method for a near space aerocraft model, which is disclosed by the invention, is mainly applied to the design of the near space aerocraft.

The invention discloses a method and application of knocking out a BnMAX1 gene in Brassica napus by a CRISPR-Cas9 system, two sgRNAs of specifically targeting Brassica napus BnMAX1 gene are designed and synthesized into oligo dimer, are connected with Cas9 vector, and introduced into hypocotyl callus of Brassica napus by Agrobacterium tumefaciens-mediated transformation to regerated into shoots. Cas9 nuclease was guided by sgRNA to cleave target sequence. Each sgRNA could pass through CRISPR-Cas9 system mediates the cleavage of BnMAX1 gene in A and C genomes, and achieves the goal of gene knockout. Phenotypic identification showed that homozygous mutant lines increased the number of branches and pods per plant, decreased plant height and increased yield.

The invention relates to a data information acquisition method, a data analysis method, a data information acquisition device, a data analysis device and a non-transitory computer readable storage medium. The data information acquisition method is applied to a database of target diseases. The data information acquisition method comprises the following steps: acquiring one or more clinical data corresponding to a data element based on an extraction path of the clinical data corresponding to the data element; based on the patient information, associating one or more clinical data according to the corresponding patient information; obtaining standard data in the corresponding data element based on the corresponding relationship between the data element and the clinical data; and performing associated storage on each standard data corresponding to the same patient information and each data element corresponding to each standard data. Through the data information acquisition method providedby the invention, various clinical data can be integrated based on the patient information, so that useful information can be quickly extracted during research, a research process is accelerated, andmedical development is promoted.

The invention discloses a kind of LDPC encode and decode hardware emulate system basing on FPGA. The system includes the control software of PC terminal and the hardware basing on DPFA which includes the control module of PCI interface, random number generator, Gaussian noise generator, LDPC encoder / decoder and so on. The invention bases on FPGA hardware and realizes simulation study of the LDPC, at the same time, the system is good at controllability, observation and reusability, and improves the pace of simulation ( is more than 300 times higher than the pace of the simulation software), offering a good lab environment for researching the same kind of error correcting codes further.

The invention relates to the field of civil aviation air traffic control and voice recognition, in particular to an all-end-to-end Chinese and English mixed air traffic control voice recognition method and device. According to the invention, voice features are extracted in advance through a feature learning module, so that a Chinese and English mixed air traffic control voice recognition model canextract more discriminative voice features, and better adapts to voice signals in different scenes; in a processing normal form from an original voice signal to a readable instruction text, a unifiedframework is applied to solve the problem of Chinese and English mixed voice recognition, a language attribute judgment link in an existing independent recognition system can be avoided, the system architecture of mixed voice recognition is simplified, and the speech features can be more reasonably and effectively applied to the recognition of the model, so that pronunciation and meaning are accurately judged, and the recognition performance and practicability of the mixed speech are improved.

The invention discloses a traditional Chinese medicine extract with an antitumor activity. The traditional Chinese medicine extract is extracted from paliurus ramosissimus leaves. The invention also discloses a method for preparing the traditional Chinese medicine extract by alcohol extraction with an ethanol solution, and use of the traditional Chinese medicine extract in preparing a medicament for treating tumors. The traditional Chinese medicine extract has good inhibition effects on tumors.

The invention discloses a test stand for testing of an electrical-power-assisted brake system, so that problems that the result is not accurate, the input can not be adjusted based on drivers with different personalities, and the assisting rate of the test stand can not be adjusted because of offline simulation in the prior art are solved. The test stand comprises an electrical-power-assisted brake assembly, a hydraulic module, and a real-time platform. The electrical-power-assisted brake assembly consists of an electric cylinder input device and an electrical-power assisting device; the electric cylinder input device includes an input push rod; and the electrical-power assisting device includes a tooth bar. The hydraulic module consists of a reaction plate and braking main cylinders. The electric cylinder input device is inserted into a through hole with a small diameter of the tooth bar by the input push rod and is connected with the electrical-power assisting device. The reaction plate is installed in a through hole with a large diameter of the tooth bar; the right end surface of the reaction plate and the left end surface of the input push rod are in contact with and in connection with an annular end surface of a stepped through hole of the tooth bar; and the reaction plate is connected with the right end of an output push rod of the braking main cylinder at the left side. The electrical-power-assisted brake assembly and the hydraulic module are connected with a real-time platform by a line.

The invention relates to the technical field of atmospheric science and environmental science, and discloses an atmospheric carbon dioxide high-altitude detecting instrument. The atmospheric carbon dioxide high-altitude detecting instrument comprises a detecting instrument shell; a first through hole is formed in the vertical side wall of the detecting instrument shell, the first through hole is internally and fixedly connected with a first sampling tube, and a first inlet valve is fixedly connected to the end, located outside the detecting instrument shell, of the first sampling tube; and a low-concentration standard intake pipe is fixedly connected to the end, away from the first sampling tube, of the first inlet valve, a first installing barrel is fixedly connected to the inner wall ofthe first sampling pipe, a first filtering film is fixedly connected to the inner wall of the first installing barrel, and a second through hole is formed in the vertical side wall of the detecting instrument shell. The atmospheric carbon dioxide high-altitude detecting instrument can accurately obtain the vertical distribution data of carbon dioxide concentration, effectively improves the accuracy of detection, reduces the cost of monitoring the global atmospheric carbon dioxide concentration, and facilitates acceleration of the research process of work such as a climate change research and carbon trading.

The invention discloses a dual-luciferase reporter gene carrier based on a human TLR4 gene promoter region and a construction method and application of the dual-luciferase reporter gene carrier, and belongs to the field of molecular genetics. By designing a primer TPprimer 1 and a primer TPprimer 2 with the 5' tail ends including homologous arms in the upstream and downstream sequences of an interface, the -3494 sequence to the +235 sequence of a TLR4 gene are obtained through amplification, the -3494 sequence to the +235 sequence of the TLR4 gene are seamlessly cloned to NcoI loci of a dual-luciferase reporter carrier pGL3-basic through a Gibson assembling method so as to construct the dual-luciferase reporter gene carrier pGLTlr4 / -3494+235 in the promoter region at the 5' end of the humanized TLR4 gene. The dual-luciferase reporter gene carrier can be digested through restriction enzymes such as BglII, KpnI and EcoRI and then reconnected to construct the derivative reporter gene carrier including promoters with different lengths, thus the derivative reporter gene carrier is used for analyzing the promoter sequence of the TLR4 gene in cells of different tissue sources, and specificity regulating and controlling mechanisms for promoter cells of the TLR4 gene and the like are investigated.

The invention provides an attack and defense confrontation simulation system. The system comprises an attack calculation module, a defense calculation module and a scenario evaluation module. The attack calculation module determines preliminary attack situation information based on the attack weapon parameters, receives attack adjustment feedback information generated based on the initial environment parameters and the situation information of the two parties, and adjusts the attack situation of the next moment; the defense calculation module determines preliminary defense situation information based on the defense system parameters, receives defense adjustment feedback information generated based on the initial environment parameters and the situation information of the two parties, and adjusts the defense situation of the next moment; and the scenario evaluation module generates attack adjustment feedback information and defense adjustment feedback information, and updates the attackadjustment feedback information and the defense adjustment feedback information according to the adjusted preliminary attack situation and preliminary defense situation. Thus, whether the weapon system performance meets the design index or not can be checked in the environment of simulating the real battlefield, calculation-in-loop real-time demonstration and verification are achieved, and the design process is accelerated.

The invention relates to an aluminum anode oxidation dual-test-piece experiment device which comprises a DC (direct-current) stabilized power supply and a glass container, wherein the glass container are symmetrically provided with two combined lead electrodes; the middle part of the glass container is provided with two anode oxidation test pieces; two negative electrode leads out of a DC stabilized power supply negative electrode terminal are respectively connected to the middle parts of the upper surfaces of the two combined lead electrodes; and two positive electrode leads out of a DC stabilized power supply positive electrode terminal are respectively connected with upper ends of the two anode oxidation test pieces. The experiment device has the most obvious characteristic that dual test pieces can be tested simultaneously, so that the speed and efficiency for preparing test pieces are greatly improved, the research process is accelerated greatly, and limited experiment resources can be saved.

The invention discloses a multi-span rotor test bench with adjustable shaft sections and supports. The bench comprises a drive motor, a drive shaft module, a sliding groove guide positioning module, amovable support module and a self-centering disc rotor module which are arranged on a base; the drive shaft module includes a plurality of drive shafts and a coupling, and the sliding groove guide positioning module includes a sliding block assembly sliding along a linear guide rail; the movable support module includes a self-centering sleeve in corresponding connection with the sliding block assembly through a support assembly, and the self-centering sleeve achieves locking fixing with the drive shafts by a locking assembly; the self-centering disc rotor module includes a counterweight discwhich is locked fixedly with the drive shafts by a locking assembly. The bench has the advantages of simple structure, convenient operation and reliable test, can effectively ensure high coaxiality between the shaft sections, carries out experimental researches of variable support position, variable shaft section length, variable shaft section outer diameter and the like, provides a practical andefficient rotor test system and greatly accelerates the research process.

The invention discloses a type 3 duck hepatitis A virus mutation gene ISA-A117C-T1142A and a construction method. According to the mutant gene ISA-A117C-T1142A, 117 nucleotide of a type 3 duck hepatitis A virus virulent strain genome 5'UTR is mutated from A to C, and 1,142<nd> nucleotide is mutated from T to A, so that 164 amino acid of a virus VP0 protein is mutated from tyrosine of a parent strain to asparagine. The mutant gene virus strain has the same antigenicity as the parent strain, and has higher proliferation efficiency and virus titer on duck embryos than the parent strain, and can proliferate on chicken embryos with good immunogenicity and genetic stability. The pathogenicity of the mutant gene virus strain on ducklings is significantly reduced. The mutant gene virus strain can replicate in the ducklings but does not cause disease to the ducklings, and the important foundation is laid for a pathogenic mechanism of the virus and the development of vaccines. To sum up,the type 3 duck hepatitis A virus mutation gene ISA-A117C-T1142A virus strain is an ideal vaccine candidate strain, can be used for the study of the host tropism and attenuated mechanism of type 3 duck hepatitis virus, and has broad application prospects.

The invention belongs to the field of plant biology, and relates to a method for determining the sex of an oil palm immature inflorescence by a cytohistology technique. The method comprises the steps: selecting an inflorescence in III period of a male and female non-dominant period, carrying out fixation, dehydration and transparence, wax dipping and embedding, slicing and baking, and dewaxing and staining to prepare a section, observing and photographing the section with a microscope, calculating the number of flower bract primordia, the number of flower bracts, the number of new flower bract primordia and the number of floral primordium meristems, and then determining the sex of the inflorescence according to a determining ruler of the sex of the oil palm immature inflorescence. According to the difference of oil palm male and female inflorescences in morphology and cytohistology levels, the sex of the inflorescence is determined in the male and female non-dominant period in advance by using the cytohistology technique, the research time is effectively shortened, the research process is accelerated, the basis is provided for further researching the relationship of an oil palm inflorescence sex differentiation mechanism with external factors, and important significance on flowering phase and sex ratio regulation, yield increasing and the like in oil palm production is achieved.

The invention discloses a YOLOv5-based side-scan sonar ocean sediment identification method and device, and the method comprises the steps: obtaining ocean sediment original data based on side-scan sonar, and obtaining an ocean sediment image based on side-scan sonar after analysis and reconstruction; marking the data set according to the type of the ocean substrate; constructing an ocean sediment recognition model based on a YOLOv5 target recognition algorithm, and performing model training; a plurality of different network model variants are added into the ocean sediment recognition model; in the model training process, a multi-scale training method is added; adding a transfer learning method; replacing the SGD optimization function with an Adam optimization function; and identifying a to-be-detected ocean sediment image by using the trained model to obtain an identification result. According to the method, the effect of real-time target detection on the video file can be achieved, the detection efficiency is improved, and development of actual ocean exploration related work is facilitated.

The invention discloses a primer for simultaneously detecting three viruses of CMV, SMV and BCMV in soybeans and a detection method thereof. The primer comprises the following steps: (1), designing the primer with primer sequences shown in SEQ ID NO. 1-6; (2), extracting soybean leaf RNA, and preparing cDNA through reverse transcription; (3), with the cDNA obtained in the step (2) as a template, performing multiplex PCR detection by using the primer in the step (1). The primer designed by the invention is strong in specificity and high in sensitivity, the detection speed of soybean viruses isgreatly increased, and especially when the number of samples to be detected is large, a great convenience is provided for solving the problems of great soybean yield reduction or even no yield causedby the soybean viruses and the like.

The invention provides spark plasma sintering equipment comprising a multi-cavity sample mold. According to the spark plasma sintering equipment, a plurality of samples can be sintered correspondingly, temperature and conditions needed by the different samples can be controlled for the different samples, and a positioning system is configured; and the problem of high-flux high-efficiency preparation of existing commercial spark plasma sintering in new material exploration is solved.

The invention discloses an attenuated JEV (Japanese encephalitis virus) having enhanced replicability, and a preparation method and application of the attenuated JEV (Japanese encephalitis virus) having enhanced replicability, and belongs to the technical field of biomedical science. The attenuated JEV disclosed by the invention is a Japanese encephalitis virus obtained in a manner that the 644th-site aspartic acid of an NS5 protein of the Japanese encephalitis virus is mutated into threonine, the 248th-site base T in a 3' noncoding region is mutated into C, the 254th-site base A is mutated into G and the 258th-site base A is mutated into G. A mutation strain obtained by the method disclosed by the invention has obviously-improved reproduction capacity, and besides, the pathogenicity of the mutation strain is reduced, so that when the mutation strain is used for vaccine production, the yield can be increased, and the cost can be reduced. The time period for obtaining the mutation strain by the method disclosed by the invention is short, operations are simple and convenient, and a process for incubating attenuated strains and researching a pathogenic mechanism of viruses can be accelerated.

The invention discloses a method for identifying protein phosphorylation sites, and relates to the field of molecular biology technology. Specifically, the method for identifying protein phosphorylation sites includes separating the protein expressed by expression vector 1 through immunoprecipitation experiments , using phosphorylated antibodies to perform immunoblotting experiments to detect the modification of the phosphorylation site to be tested, the expression vector 1 contains an amino acid sequence fragment with the site to be tested, which is under the condition of no phosphorylated antibody against a single protein site , can also effectively and accurately detect the phosphorylation modification of a single site of the protein, save the cost of preparing phosphorylated antibodies for specific sites, and speed up the research process of protein phosphorylation sites.

The invention discloses a multi-span rotor test bench with adjustable shaft sections and supports, which comprises a driving motor arranged on a base, a transmission shaft module, a chute guiding and positioning module, a movable supporting module, and a self-centering disc rotor module The drive shaft module includes a number of drive shafts and couplings, the chute guide positioning module includes a slider assembly that slides along a linear guide rail; the movable support module includes a self-centering that is connected to the slider assembly through a support assembly The self-centering sleeve is locked and fixed to the transmission shaft through a locking assembly; the self-centering disk rotor module includes a counterweight disk that is locked and fixed to the transmission shaft through a locking assembly. The present invention has the characteristics of simple structure, convenient operation, reliable test, etc., can effectively guarantee high coaxiality between each shaft section, and can carry out experimental researches such as variable support position, variable shaft section length, variable shaft section outer diameter, etc. A practical and efficient rotor test system is provided, which greatly speeds up the research process.

The invention discloses a type 3 duck hepatitis A virus mutant gene ISA-T1142A and its construction method. The 3403rd nucleotide G of the mutant gene genome is mutated into T, which can be used as a genetic marker for infectious clones and can be used to distinguish rescued strains With the parental strain and the wild strain; the 1142nd nucleotide is mutated from T to A, so that the 164th amino acid of the virus VPO protein is mutated from the tyrosine of the parental strain to asparagine. The mutant gene virus strain has the same antigenicity as the parent strain, and has higher proliferation efficiency and virus titer than the parent strain on duck embryos, and has good immunogenicity and genetic stability. The pathogenicity of the mutant gene virus strain to ducklings has been significantly reduced, which has laid an important foundation for the pathogenic mechanism of the virus and the development of vaccines. In conclusion, the type 3 duck hepatitis A virus mutant gene ISA‑T1142A virus strain can be used as a vaccine candidate or strain and can be used for the study of the attenuation mechanism of type 3 duck hepatitis A virus, with broad application prospects.

The invention relates to the technical fields of atmospheric science and environmental science, and discloses an atmospheric carbon dioxide high-altitude detector, comprising a detector casing, wherein a vertical side wall of the detector casing is provided with a first through hole, and the first through hole is opened in the vertical side wall of the detector casing. A first sampling tube is fixedly connected in the hole, the end of the first sampling tube located outside the detector shell is fixedly connected with a first intake valve, and the end of the first intake valve away from the first sampling tube is fixedly connected with a low-pressure valve. Concentration standard intake pipe, the inner wall of the first sampling pipe is fixedly connected with a first installation cylinder, the inner wall of the first installation cylinder is fixedly connected with a first filter membrane, and the vertical side wall of the detector housing is provided with second through hole. The atmospheric carbon dioxide high-altitude detector can accurately obtain the vertical distribution data of carbon dioxide concentration, effectively improve the detection accuracy, reduce the cost of monitoring the global atmospheric carbon dioxide concentration, and help improve climate change research, carbon trading and other work. research process.

The invention discloses a type-3 DHAV (duck hepatitis A virus) mutant gene ISA-A117C and a construction method. G of 3403th nucleotide of a mutant genome is mutated into T, serves as a genetic marker of infectious clone and can be used for distinguishing a rescue strain from a parent strain and a wild strain; A of 117th nucleotide of 5'UTR is mutated into C. A virus strain of the mutant gene has the same antigenicity as the parent strain, but has higher proliferation efficiency and virus titer than the parent strain on a duck embryo and can be proliferated on a chicken embryo, and the immunogenicity and the genetic stability are good. The pathogenicity of the virus strain of the mutant gene for ducklings is notably reduced, and the virus strain can be duplicated in the ducklings, but has no pathogenicity, which lays an important foundation for a virus pathogenic mechanism and vaccine development. To sum up, the type-3 DHAV mutant gene ISA-A117C virus strain can serve as a vaccine candidate or culture toxic strain, can be used for type-3 DHAV host tropism and attenuated mechanism research and has broad application prospects.

The invention discloses an establishment method of a tobacco hairy root genetic transformation system. Comprising the steps of sterilizing tobacco seeds to obtain sterile tobacco seeds, germinating the tobacco seeds to obtain tobacco sterile seedlings, preparing agrobacterium, preparing agrobacterium infection liquid, infecting, co-culturing, sterilizing and culturing, screening and culturing to obtain tobacco hairy roots capable of being used for gene function detection, identifying the hairy roots and the like. The method is simple and easy to implement and short in period, enough hairy roots can be obtained in 8 weeks, and the obtained hairy roots can be applied to functional identification of tobacco genes (including detection of expression quantity of transformed genes; detection of the expression quantity of other target genes, detection of the content of nicotine, nornicotine, neonicotine and other tobacco substances); the technical problem that the traditional stable tobacco plant transformation from genetic transformation to T1-generation plants capable of being used for gene function identification takes a long time of about one year is solved; compared with the prior art, the tobacco hairy root genetic transformation system obviously shortens the research period.

The invention relates to the technology of the near space aerocraft. In order to provide a system-level consistency check novel model verification system output on the basis of the dynamic time sequence of the near space aerocraft model, give qualitative model verification based on the monte carlo method, carry out risk analysis to the model, comprehensively carry out consistency check to a model to be verified and give a visual verification criterion, the invention adopts the technical scheme that the verification and simulation realization method for a near space aerocraft model comprises the following steps of: (1) on the basis of the monte carlo simulation method, carrying out qualitative verification; (2) carrying out the qualitative model verification based on a time domain analysis method; and (3) carrying out qualitative model verification based on risk analysis, taking a model parameter as a risk to give a visual qualitative model verification criterion, giving a model comparison standard when a plurality of models to be verified exist, verifying whether the model can be safely used, and giving a safe-use range. The verification and simulation realization method for a near space aerocraft model, which is disclosed by the invention, is mainly applied to the design of the near space aerocraft.

Based on first dimension chromatographic separation, the system applies multichannel chromatogram array to second chromatographic separation so that analysis efficiency is raised greatly. Multichannel chromatogram array is possible to expand to 2-50 channels. Multichannels are interfaced to first dimension chromatogram to complete separation procedure of enrichment and gradient elution from one column to another, providing effective measure for analyzing samples of protein group. Comparing with method of single channel one or two dimensions chromatogram, the invented method shortens analytical cycle, provides easy operation. Integrated assembles in the invention can be utilized in combined with laser ionization mass spectrum so as to realize automatic chromatograph-mass spectrograph for protein group.

The present invention disclosed a type 3 duck armor virus mutation gene isa‑a117c and the construction method. The 3403 -bit nucleotide of the mutant gene genome is T to T. The 117 nucleotide of 5'UTR from A and Wild strains is mutated from A to C. The mutant gene virus strain has the same antigenicity as the pro -based strain, and has higher proliferation efficiency and virus titer than the pro -pro -base strains on the duck embryo, and can be proliferated on the chicken embryo. The immunogenicity and genetic stability are good. The pathogenic force of mutant gene virus strains has fallen significantly to ducky ducks. It can be replicated in the ducky duck, but it does not cause disease to the duck. This has laid an important foundation for the virus pathogenic mechanism and vaccine development. In summary, the type 3 duck noreal virus mutation gene isa‑a117C virus strains can be used as a vaccine candidate or cultivated strain and can be used for the research of type 3 duck nail virus hosts and weak mechanisms. The application prospects are broad.

The orthogonal process for finding the effective chemical parts or components in compound Chinese medicine is disclosed, and the orthogonal process is superior to available physical and chemical analysis process. The orthogonal process includes water steam distillation to extract volatile oil, water decoction for twice or more times, concentration, filtering and other steps. The present invention is used for the separation and determination of effective components in Chinese medicine and preparation process Chinese medicine.