45results about How to "Speed up the research process" patented technology

The invention discloses a method and application of knocking out a BnMAX1 gene in Brassica napus by a CRISPR-Cas9 system, two sgRNAs of specifically targeting Brassica napus BnMAX1 gene are designed and synthesized into oligo dimer, are connected with Cas9 vector, and introduced into hypocotyl callus of Brassica napus by Agrobacterium tumefaciens-mediated transformation to regerated into shoots. Cas9 nuclease was guided by sgRNA to cleave target sequence. Each sgRNA could pass through CRISPR-Cas9 system mediates the cleavage of BnMAX1 gene in A and C genomes, and achieves the goal of gene knockout. Phenotypic identification showed that homozygous mutant lines increased the number of branches and pods per plant, decreased plant height and increased yield.

The invention relates to a data information acquisition method, a data analysis method, a data information acquisition device, a data analysis device and a non-transitory computer readable storage medium. The data information acquisition method is applied to a database of target diseases. The data information acquisition method comprises the following steps: acquiring one or more clinical data corresponding to a data element based on an extraction path of the clinical data corresponding to the data element; based on the patient information, associating one or more clinical data according to the corresponding patient information; obtaining standard data in the corresponding data element based on the corresponding relationship between the data element and the clinical data; and performing associated storage on each standard data corresponding to the same patient information and each data element corresponding to each standard data. Through the data information acquisition method providedby the invention, various clinical data can be integrated based on the patient information, so that useful information can be quickly extracted during research, a research process is accelerated, andmedical development is promoted.

The invention discloses a traditional Chinese medicine extract with an antitumor activity. The traditional Chinese medicine extract is extracted from paliurus ramosissimus leaves. The invention also discloses a method for preparing the traditional Chinese medicine extract by alcohol extraction with an ethanol solution, and use of the traditional Chinese medicine extract in preparing a medicament for treating tumors. The traditional Chinese medicine extract has good inhibition effects on tumors.

The invention provides an attack and defense confrontation simulation system. The system comprises an attack calculation module, a defense calculation module and a scenario evaluation module. The attack calculation module determines preliminary attack situation information based on the attack weapon parameters, receives attack adjustment feedback information generated based on the initial environment parameters and the situation information of the two parties, and adjusts the attack situation of the next moment; the defense calculation module determines preliminary defense situation information based on the defense system parameters, receives defense adjustment feedback information generated based on the initial environment parameters and the situation information of the two parties, and adjusts the defense situation of the next moment; and the scenario evaluation module generates attack adjustment feedback information and defense adjustment feedback information, and updates the attackadjustment feedback information and the defense adjustment feedback information according to the adjusted preliminary attack situation and preliminary defense situation. Thus, whether the weapon system performance meets the design index or not can be checked in the environment of simulating the real battlefield, calculation-in-loop real-time demonstration and verification are achieved, and the design process is accelerated.

The invention relates to an aluminum anode oxidation dual-test-piece experiment device which comprises a DC (direct-current) stabilized power supply and a glass container, wherein the glass container are symmetrically provided with two combined lead electrodes; the middle part of the glass container is provided with two anode oxidation test pieces; two negative electrode leads out of a DC stabilized power supply negative electrode terminal are respectively connected to the middle parts of the upper surfaces of the two combined lead electrodes; and two positive electrode leads out of a DC stabilized power supply positive electrode terminal are respectively connected with upper ends of the two anode oxidation test pieces. The experiment device has the most obvious characteristic that dual test pieces can be tested simultaneously, so that the speed and efficiency for preparing test pieces are greatly improved, the research process is accelerated greatly, and limited experiment resources can be saved.

The invention discloses a primer for simultaneously detecting three viruses of CMV, SMV and BCMV in soybeans and a detection method thereof. The primer comprises the following steps: (1), designing the primer with primer sequences shown in SEQ ID NO. 1-6; (2), extracting soybean leaf RNA, and preparing cDNA through reverse transcription; (3), with the cDNA obtained in the step (2) as a template, performing multiplex PCR detection by using the primer in the step (1). The primer designed by the invention is strong in specificity and high in sensitivity, the detection speed of soybean viruses isgreatly increased, and especially when the number of samples to be detected is large, a great convenience is provided for solving the problems of great soybean yield reduction or even no yield causedby the soybean viruses and the like.

The invention provides spark plasma sintering equipment comprising a multi-cavity sample mold. According to the spark plasma sintering equipment, a plurality of samples can be sintered correspondingly, temperature and conditions needed by the different samples can be controlled for the different samples, and a positioning system is configured; and the problem of high-flux high-efficiency preparation of existing commercial spark plasma sintering in new material exploration is solved.

The invention discloses an attenuated JEV (Japanese encephalitis virus) having enhanced replicability, and a preparation method and application of the attenuated JEV (Japanese encephalitis virus) having enhanced replicability, and belongs to the technical field of biomedical science. The attenuated JEV disclosed by the invention is a Japanese encephalitis virus obtained in a manner that the 644th-site aspartic acid of an NS5 protein of the Japanese encephalitis virus is mutated into threonine, the 248th-site base T in a 3' noncoding region is mutated into C, the 254th-site base A is mutated into G and the 258th-site base A is mutated into G. A mutation strain obtained by the method disclosed by the invention has obviously-improved reproduction capacity, and besides, the pathogenicity of the mutation strain is reduced, so that when the mutation strain is used for vaccine production, the yield can be increased, and the cost can be reduced. The time period for obtaining the mutation strain by the method disclosed by the invention is short, operations are simple and convenient, and a process for incubating attenuated strains and researching a pathogenic mechanism of viruses can be accelerated.

The invention discloses a type-3 DHAV (duck hepatitis A virus) mutant gene ISA-A117C and a construction method. G of 3403th nucleotide of a mutant genome is mutated into T, serves as a genetic marker of infectious clone and can be used for distinguishing a rescue strain from a parent strain and a wild strain; A of 117th nucleotide of 5'UTR is mutated into C. A virus strain of the mutant gene has the same antigenicity as the parent strain, but has higher proliferation efficiency and virus titer than the parent strain on a duck embryo and can be proliferated on a chicken embryo, and the immunogenicity and the genetic stability are good. The pathogenicity of the virus strain of the mutant gene for ducklings is notably reduced, and the virus strain can be duplicated in the ducklings, but has no pathogenicity, which lays an important foundation for a virus pathogenic mechanism and vaccine development. To sum up, the type-3 DHAV mutant gene ISA-A117C virus strain can serve as a vaccine candidate or culture toxic strain, can be used for type-3 DHAV host tropism and attenuated mechanism research and has broad application prospects.

The invention discloses an establishment method of a tobacco hairy root genetic transformation system. Comprising the steps of sterilizing tobacco seeds to obtain sterile tobacco seeds, germinating the tobacco seeds to obtain tobacco sterile seedlings, preparing agrobacterium, preparing agrobacterium infection liquid, infecting, co-culturing, sterilizing and culturing, screening and culturing to obtain tobacco hairy roots capable of being used for gene function detection, identifying the hairy roots and the like. The method is simple and easy to implement and short in period, enough hairy roots can be obtained in 8 weeks, and the obtained hairy roots can be applied to functional identification of tobacco genes (including detection of expression quantity of transformed genes; detection of the expression quantity of other target genes, detection of the content of nicotine, nornicotine, neonicotine and other tobacco substances); the technical problem that the traditional stable tobacco plant transformation from genetic transformation to T1-generation plants capable of being used for gene function identification takes a long time of about one year is solved; compared with the prior art, the tobacco hairy root genetic transformation system obviously shortens the research period.

The orthogonal process for finding the effective chemical parts or components in compound Chinese medicine is disclosed, and the orthogonal process is superior to available physical and chemical analysis process. The orthogonal process includes water steam distillation to extract volatile oil, water decoction for twice or more times, concentration, filtering and other steps. The present invention is used for the separation and determination of effective components in Chinese medicine and preparation process Chinese medicine.