The invention provides a method for measuring the content of Danshensu in shenshuaining capsules by utilizing an
HPLC method, comprising the following steps: 1, weighing shenshuaining capsules into a measuring flask, crushing, adding
methanol, performing
ultrasound in water bath, placing in the ice-water bath, taking out, adding
methanol, centrifuging, and taking the supernate as the
test solution of the shenshuaining capsules; 2, weighing Danshensu
sodium comparison products, placing in the measuring flask, performing
ultrasound in water bath, taking out, placing in the ice-water bath, taking out, adding
methanol to
shake up for being served as the comparison products solution; 3, leading the
chromatographic column to be Inertsil ODS-3 (4.6X 150mm 5mu m), the mobile phase to be methanol-water-glacial
acetic acid, the volume ratio to be 19:80:1, the flow velocity to be 1.0ml / min, the detected
wavelength to be 281nm, the sensitivity to be 0.01AUFS, the sample size to be 10 mu L, the
column temperature to be 40 DEG C, and the theoretic plate number to be no less than 5000 calculated by chromatographic peak of the Danshensu; and 4, injecting Danshensu
sodium comparison products solution and shenshuaining capsules
test solution into liquid chromatograph, and measuring the chromatographic peak presented by shenshuaining capsules
test solution with the same chromatographic retention as the Danshensu
sodium comparison products solution. The invention provides a new
comparison standard for accurately assessing the quality of shenshuaining capsules, thus strengthening
controllability of product quality of shenshuaining capsules and being beneficial for comprehensive monitoring of product quality of shenshuaining capsules.