Method for determining sex of oil palm immature inflorescence by cytohistology technique

Abstract

The invention belongs to the field of plant biology, and relates to a method for determining the sex of an oil palm immature inflorescence by a cytohistology technique. The method comprises the steps: selecting an inflorescence in III period of a male and female non-dominant period, carrying out fixation, dehydration and transparence, wax dipping and embedding, slicing and baking, and dewaxing and staining to prepare a section, observing and photographing the section with a microscope, calculating the number of flower bract primordia, the number of flower bracts, the number of new flower bract primordia and the number of floral primordium meristems, and then determining the sex of the inflorescence according to a determining ruler of the sex of the oil palm immature inflorescence. According to the difference of oil palm male and female inflorescences in morphology and cytohistology levels, the sex of the inflorescence is determined in the male and female non-dominant period in advance by using the cytohistology technique, the research time is effectively shortened, the research process is accelerated, the basis is provided for further researching the relationship of an oil palm inflorescence sex differentiation mechanism with external factors, and important significance on flowering phase and sex ratio regulation, yield increasing and the like in oil palm production is achieved.

Description

technical field [0001] The invention belongs to the field of plant biology, and relates to a method for judging the sex of inflorescences, in particular to a method for judging the sex of young oil palm inflorescences by cytohistological technology. Background technique [0002] The sexual differentiation of plant inflorescence is an extremely important stage in the development of flowering plants, a key link in the continuation of plant life, a complex morphogenesis process, and a special organogenesis phenomenon. Understanding the sex differentiation process of plant inflorescences, including changes in cell histological characteristics and related endogenous hormone content, is helpful for understanding its developmental biology and reproductive biology characteristics, realizing the regulation of flowering period and sex ratio in production, hybridization, utilization of heterosis, etc. is of great significance. [0003] Oil palm is an important woody oil crop. Its oil ...

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Problems solved by technology

[0005] The winter temperature in southern my country is relatively cold, and the locally cultivated oil palm is adaptable to the environment, which is significantly different from the oil palm cultivated near the equator. However, there has been no relevant report on the cytohistological study of its inflorescence sex differentiation in China so far.

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