Sperm antibody testing reagent

A technology of anti-sperm antibody and detection reagent, applied in the field of anti-sperm antibody detection reagent, can solve the problems of immune infertility diagnosis, treatment deviation, background noise, anti-sperm false positive, etc., and achieve easy results, simple detection and specificity strong effect

Inactive Publication Date: 2003-07-30
ANHUI ANKE BIOTECHNOLOGY (GRP) CO LTD
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AI Technical Summary

Problems solved by technology

At present, whether it is imported kits or domestic kits used in China, most of them are ELISA reagents. ELISA detection of anti-sperm antibodies mainly has the following shortcomings: 1. Due to the existence of soluble FC receptors in seminal plasma on the surface of sperm, the test results have serious background noise
2. The detected antisperm antibodies are free antibodies in body fluids

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0035] The anti-sperm antibody detection reagent is composed of the following components in weight ratio:

[0036] 2.5% sensitized sheep red blood cell suspension 20ul

[0037] 1:2 9 Rabbit anti-human IgG 20ul

[0038] The method of making the above-mentioned anti-sperm antibody detection reagent:

[0039] A. Production process of 2.5% sensitized sheep red blood cell suspension

[0040] 1. Choose 1 healthy young sheep, take 50ml blood without jugular vein, store the blood in Alsever solution at 4℃, and shake it well.

[0041] 2. Take 50ml of the above fresh sheep red blood cell solution and wash it 3 times in a centrifuge with physiological saline, and the process condition is 500 rpm.

[0042] Three, hydroformylation treatment

[0043] Take PH7.2 phosphate buffer to make a 5% suspension, slowly drop 2.5% glutaraldehyde on a shaker, and continue to shake for 60 minutes after the addition;

[0044] Wash the above sheep red blood cell suspension 5 times with physiological saline in ...

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Abstract

The sperm antibody testing reagent includes sensitized sheep erythrocyte suspension of 2-3 % concentration and rabbit antihuamn IgG in the concentration of 1 to 2 to the (8-10)-th power. The sensitized sheep erythrocyte suspension of 2-3% concentration is prepared through taking sheep blood, washing, hydroformylation, tanning, sensitizing and other steps. The preparation of rabbit antihuman IgG in the concentration of 1 to 2 to the (8-10)-th power includes taking rabbit blood, separating blood serum and other steps. The reagent has strong specificity, high detection sensitivity, no false positive and no false negative phenomenon, high stability, and storage life as long as one year. Using the reagent can obtain easy-to-judge detection result sipmle and fast.

Description

Technical field [0001] The invention relates to an anti-sperm antibody detection reagent. Background technique [0002] Immune factors are one of the causes of male infertility. According to the classification of the causes of human infertility by WHO, the immune factors in the causes of infertility are defined as: normal sex and ejaculatory function, in at least one semen specimen, mixed antiglobulin reaction test (MAR) or Immune Bead Test (IBT) No less than 50% of the motile sperm surface is coated with antibodies. In 2000, it was clearly stipulated that only this test can be used as the diagnostic criteria for immune infertility in the diagnosis of infertility. [0003] The domestic anti-sperm antibody detection mainly uses the classic agglutination test, immobilization test and enzyme immunoassay (ELISA). The latter is coated with intact sperm or purified sperm antigen after death, and the enzyme-labeled anti-human antibody is used with the corresponding substrate.物色。 The obj...

Claims

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Application Information

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IPC IPC(8): G01N33/50G01N33/563
Inventor 徐振山杨玲杜贤宇李平生宋礼华
Owner ANHUI ANKE BIOTECHNOLOGY (GRP) CO LTD
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