A kind of Mycoplasma bovis antibody detection reagent and preparation method thereof

A technology for detection of mycoplasma bovis and antibodies, which is applied in botany equipment and methods, biochemical equipment and methods, biological testing, etc., can solve the problems of unpublished methods, insufficient specificity and complicated operation steps of the whole bacterial protein method, and achieve Avoid the effects of short storage time, easy long-term storage, and high hemagglutination titer
CN104062439BActive Publication Date: 2016-06-08LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
Publication Date
2016-06-08

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Abstract

The invention relates to a biological detection reagent and a preparation method thereof, and particularly relates to a detection reagent for a mycoplasma bovis antibody and a preparation method thereof. The detection reagent for the mycoplasma bovis antibody is composed of one part of a mycoplasma bovis P48 recombined and expressed fusion protein antigen and one part of 10% hydroformylation-tanned sheep red blood cells (v / v) which are uniformly mixed. The gene sequence of the P48 recombined and expressed fusion protein antigen for the detection reagent for the mycoplasma bovis antibody is SEQ ID No.1. The detection reagent can be used for really detecting mycoplasma bovis and has the advantages of long preservation time, high sensitization titer, easiness in long-time preservation, high blood coagulation titer, simplicity in operation, convenience and rapidness, low cost, safety and stability, no toxin, no environmental pollution and the like.
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Description

technical field

[0001] The invention relates to a biological detection reagent and a preparation method of the detection reagent, specifically, the present invention relates to a detection reagent of Mycoplasma bovis antibody and a preparation method thereof. Background technique

[0002] Mycoplasma bovis is an important pathogenic pathogen that infects cattle. Hale first isolated the pathogen from the milk of mastitis in the United States in 1961. It has been confirmed that Mycoplasma bovis not only causes bovine pneumonia and mastitis, but also causes arthritis, keratoconjunctivitis, otitis, reproductive tract inflammation, miscarriage and infertility. Since 2008, an outbreak of "contagious Mycoplasma bovis pneumonia" characterized by necrotizing pneumonia has occurred in newly imported beef cattle in some areas of my country. It has caused huge economic losses to my country's cattle industry. Since then, clinical diagnosis has been reported in Gansu, Ningxia, Qinghai, Gu...

Claims

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