34 results about "Erythrocyte antibody" patented technology

The invention relates to a method for detecting a plurality of antigenic molecules carried by erythrocytes and/or a plurality of anti-erythrocyte antibodies of an individual, comprising bringing a sample into contact with distinguishable beads, on which are attached a) antibodies specific for said antigens, or b) erythrocytes, erythrocyte membrane fragments or blood group antigens.

The invention relates to an SPA-antibody tripolymer, a cell treating kit containing the tripolymer, a preparation method and application thereof. The tripolymer comprises an anti erythrocyte antibody, an SPA and an antibody of a certain anti leukocyte antigen or other antigens. The tripolymer is combined with an erythrocyte through the anti erythrocyte antibody per se, the other antibody is combined with a corresponding leukocyte antigen (or other antigens), and then leukocyte (other cells, factors) and the erythrocyte are deposited through an erythrocyte sedimentation process or other methods of density gradient centrifugation and the like, thereby achieving the purpose of eliminating ingredients of corresponding cells and the like, connecting the erythrocyte with a corresponding antigen by the SPA, and being used as a mean for detecting a certain antigen. The invention has convenience and simpleness, and can be directly used for clinically separating and extracting stem cells/progenitor cells; and the collected and extracted stem cells/progenitor cells have no external markers. Meanwhile, the kit based on the tripolymer can be industrially produced so as to realize the popularization of the separation and purification technology of hematopoietic stem cells.

The invention discloses a blood group antigen chip and application thereof in detection of unexpected erythrocyte antibodies. The detection comprises: (1) coating a carrier with a blood group erythrocyte membrane antigen; (2) contacting a tested sample with the antigen of the carrier; (3) adding a second antibody labeled with a marker; and (4) performing signal detection on the marker. The blood group antigen chip and a detection method of unexpected erythrocyte antibodies are high in sensitivity, good in accuracy, independent of erythrocytes, and capable of detecting binding antibodies of a plurality of rare blood group antigens.

A process for the detection of antibodies in a test sample by carrying out the following steps:

• (a) preparing a suspension of erythrocytes with a test serum or plasma by mixing a test serum or plasma with erythrocytes;
• (b)incubating the suspension formed in step (a) at a temperature of from 37° C. to 45° C. for a period of time from sixty seconds to 10 minutes to bind any antibodies in the test serum or plasma to the surface of said erythrocytes
• (c)combining said suspension with an amount of a solution of a macromolecule which is effective for agglutination of said erythrocytes;
• (d) packing the resultant red cell agglutinates by centrifugation from the suspension of step (c); and,
• (f) determining the presence of anti-erythrocyte antibodies by observing if antibody-dependent erythrocyte aggregation has occurred.

The invention relates to a kit and use of SPA (staphylococcal protein A)-antibody trimer. The antibody trimer is formed by an anti erythrocyte antibody, SPA and antibodies of some anti-leukocyte antigen or other antigens. According to the antibody trimer, the self anti erythrocyte antibody is combined with erythrocyte, the other antibody is combined with corresponding leukocyte antigen (or other antigen), then leukocyte (or other cell, factor) and erythrocyte are deposited by a blood sedimentation process or methods, such as density gradient centrifugation to achieve the purpose of eliminating components of corresponding cell and the like, and the erythrocyte can be also connected with corresponding antigen by virtue of the SPA to be used as means for detecting certain antigens. The kit is convenient and simple and can be directly used for clinical separation and extraction of stem cells/progenitor cells, and the collected and extracted stem cells/progenitor cells do not have any foreign markers. The kit based on the trimer can be industrially produced to realize popularization of human hematopoietic stem cell separation and purification technology.

The invention relates to a piperacillin induced hemolysis detection reagent kit and a method for preparing the same. The piperacillin induced hemolysis detection reagent kit comprises piperacillin inducible hemolysis detection reagent cards I, piperacillin inducible hemolysis detection reagent cards II, piperacillin treated red blood cells, non-piperacillin treated red blood cells, antibody positive control liquid and antibody negative control liquid. The piperacillin treated red blood cells are normal O Rh (rhesus) negative red blood cells treated by piperacillin medicine solution; the non-piperacillin treated red blood cells are normal O Rh negative red blood cells with the concentration of 2-2.5%; the piperacillin antibody positive control liquid is piperacillin antibody positive serum; the piperacillin antibody negative control liquid is piperacillin antibody negative serum. The piperacillin induced hemolysis detection reagent kit and the method have the advantages that the piperacillin inducible hemolysis detection reagent kit is high in sensitivity, good in repeatability and stable in quality, detection procedures are short in time consuming, the method is simple, convenient and feasible, and results are easy to judge.

The invention discloses test paper for quantitatively determining urea and a preparation method of the test paper, and belongs to the field of in-vitro diagnosis. The test paper comprises a diffusionlayer, a blood filtering layer, a reaction layer and a hydrophobic breathable color developing layer, wherein the blood filtering layer comprises buffer salt, divalent metal ions, polyvinylpyrrolidone, hemagglutinin or an anti-erythrocyte antibody, and the reaction layer comprises buffer salt, a surfactant, divalent metal ions, urease, polysaccharide, ascorbic acid oxidase, polyol, polyvinylpyrrolidone and an enzyme protective agent; the color developing layer contains an alcohol and a color developing agent. A blood filtering solution, a reaction solution and a color developing agent solutioncontaining related components are prepared, a blood filtering layer and a reaction layer material are soaked in the blood filtering solution and the reaction solution respectively, the color developing agent solution is sprayed on a hydrophobic breathable color developing layer material and dried to obtain a blood filtering layer, a reaction layer and a hydrophobic breathable color developing layer, and the blood filtering layer, the reaction layer and the hydrophobic breathable color developing layer are assembled with other components to obtain the test paper. The test paper provided by theinvention can simply, rapidly and quantitatively detect the content of urea in whole blood, plasma and serum.

The invention belongs to a method for whole blood quick determination of homocysteine. The method comprises the following steps: (1) filtering whole blood by a filter material to filter red cells in the whole blood; (2) performing absorption processing on the filtered whole blood by anti-erythrocyte antibodies to adsorb unfiltered red cells; (3) reducing homocysteine in the whole blood subjected to the absorption processing by a reducing agent into reduced homocysteine, and then performing pyrolysis on the reduced homocysteine by homocysteine lyase to generate H2S; (4) performing a fluorescence reaction on the H2S and a color developing agent and finally reading a result. The method needs no special separation device, can remove the red cells in the whole blood by the filter material and the anti-erythrocyte antibodies, and is good in removing effect, thus ensuring the testing accuracy and greatly simplifying the operation.

The invention provides a use of a heat shock protein gp96 in treatment on autoimmune hemolytic anemia and belongs to the technical field of protein engineering and biomedical application. The amino acid sequence of the heat shock protein gp96 is shown in the formula of SEQ ID NO. 2 or is a specific fragment of the amino acid sequence shown in the formula of SEQ ID NO. 2. The heat shock protein gp96 can induce the production of regulatory T cells, can reduce anti-erythrocyte antibody titer, can improve the number of red blood cells and content of hemoglobin, can be used for preparation of a drug for treating autoimmune hemolytic anemia and has a wide application prospect.

The invention relates to a rapid immune detection method and a detection kit for a blood sample. The method comprises the following step of: removing erythrocyte from the sample. The step of removing the erythrocyte is that: the whole blood sample is enabled to flow through a solid phase carrier which is coated and fixed with an anti erythrocyte antibody or an active section of the anti erythrocyte antibody; in the process that the sample flows through the solid phase carrier, the erythrocyte is combined with the anti erythrocyte antibody or the active section of the anti erythrocyte antibody, so that the erythrocyte is retained in the solid phase carrier; and the solid phase carrier consists of a water absorption medium which is subjected to sealing treatment. By the rapid immune detection method and the detection kit for the blood sample, the erythrocyte in the blood sample is effectively retained; and compared with the prior art, the invention has the advantages that: the detection can be directly performed by taking the whole blood as the sample, the limitation of a blood serum test method which only takes blood serum as a sample is overcome, blood volume and detection time are saved, and the detection sensitivity is high.

The invention provides an anti-interference film and a liver function combined test strip. The anti-interference film is obtained by wetting and treating a substrate with anti-interference treatment fluid. The anti-interference treatment fluid comprises 10-100KU/L of pyruvic oxidase, 100-500KU/L of catalase, 3-30 micronM of cupric salt and 0.1-0.3g/L of erythrocyte antibody. The liver function combined test strip comprises a diffusion film, the anti-interference film, a blood filter film, a reaction film, a color development film and a bottom board, which are stacked up in sequence. The liverfunction combined test strip prepared by the anti-interference film can detect ALT and AST two substances simultaneously and can obtain ALT, AST and ALT/AST three indicators, thereby providing more reliable guidance for clinical diagnosis; and the test strip is simple and fast, adopts smaller matched instruments and is flexible and wide in application.

The present invention relates to an in-vitro diagnosis device for detecting and/or identifying antigen and/or antibody from a sample of biological fluid, particularly from a sample of blood or sample of blood components. The invention also relates to different uses of this device such as the detection and/or the identification of red blood cells antigens, platelet antigens, viralantigens, bacterial antigens, parasite antigens, the detection and/or the identification of anti-red blood cells antibodies, antiplatelet antibodies, antiviral antibodies, antibacterial antibodies and antiparasitic antibodies.

The invention discloses a filtering membrane pretreatment liquid for improving immunofluorescence detection sensitivity and a preparation method and application thereof. The filtering membrane pretreatment liquid for improving the immunofluorescence detection sensitivity comprises a solvent and solute, the solvent is a buffer solution Tris-HCL, the concentration of the Tris-HCL is 50-100 Mm/L, the solute comprises 2-6 g/L of a surfactant, 1-5 g/L of PVP, 3-6 g/L of PEG6000, 20-60 g/L of saccharides, 30-60 g/L of inert protein, 1-5 mg/L of heterophagy antibody, 1-5 mg/L of anti-erythrocyte antibody and 0.5-1 g/L of preservative. The filter membrane pretreatment liquid has the advantage of improving the detection sensitivity of the micro-fluidic chip.

The present invention relates to an immunochromatographic test strip, which comprises: a sample pad; a marker pad, which is connected to the sample pad; a coating pad, which is connected to the markerpad, and is equipped with a detection line and a quality control line sequentially; and a water absorption pad, which is connected to the coating pad; wherein the sample pad comprises a glass fiber pad, and an RBC antibody compound is sprayed on the glass fiber pad. The invention also provides a device comprising the immunochromatographic test strip. The invention relates to the immunochromatographic test strip and the device comprising the same, an RBC antibody in the RBC antibody compound and red blood cells are subjected to immunoaffinity reaction to form a cross-linked substance of "red blood cell-RBC antibody compound-red blood cell", compared with a single RBC antibody, the RBC compound is larger in size and more in red blood cell binding sites, so that the RBC compound is easier tobind with red blood cells and is intercepted on the sample pad.

The invention discloses a kit for detecting thymidine kinase 1 in whole blood and a detection method, belongs to the technical field of immunodetection, and solves the technical problem of long time consumption of an existing detection method. The kit comprises a reagent R1, a reagent R2 and a reagent R3, wherein the R1 reagent comprises a magnetic separation reagent and an anti-erythrocyte antibody; the R2 reagent comprises a TK1 monoclonal antibody labeled by horse radish peroxidase; the R3 reagent comprises a TK1 monoclonal antibody marked by Acridan; the method comprises the following steps: S1, adding a reagent R4 and a reagent R1 into human whole blood, reacting, and carrying out magnetic separation to obtain a reaction solution A; s2, adding a reagent R2 and a reagent R3 into the reaction solution A, and incubating to obtain a reaction solution B; and S3, adding an excitation liquid separant into the reaction liquid B, and determining. The kit and the method are used for detecting the TK1 content in human whole blood, and have the advantages of high detection efficiency and less time consumption.

The invention relates to an antibody to a red blood cell for use in treating or preventing an inflammatory disorder, and to methods of treating or preventing an inflammatory disorder comprising administering to a subject in need thereof a therapeutically effective amount of an antibody to a red blood cell.

The invention discloses a kit for detecting calcium ossein and a detection method, belongs to the technical field of immunodetection, and solves the problem of long time consumption of related detection methods. The kit comprises a calibrator, normal saline, a reagent R1, a reagent R2 and a reagent R3, the R1 reagent comprises a magnetic separation reagent and an anti-erythrocyte antibody; the R2 reagent comprises a BGP monoclonal antibody marked by HRP (horse radish peroxidase); the R3 reagent comprises a BGP monoclonal antibody marked by Acridan; the method comprises the following steps: S1, adding normal saline and an R1 reagent into human whole blood, reacting, and carrying out magnetic separation to obtain a reaction solution A; s2, adding a reagent R2 and a reagent R3 into the reaction solution A, and incubating to obtain a reaction solution B; s3, adding excitation liquid and spacer liquid into the reaction liquid B, and measuring; and S4, drawing a standard curve, and determining the BGP content. The kit and the method are used for detecting the BGP content in human whole blood, and have the advantages of high detection efficiency and less time consumption.