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Rapid immune detection method and detection kit for blood sample

A whole blood sample and sample technology, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve problems such as rapid diagnosis and inability to use whole blood, and achieve the goal of overcoming limitations, saving blood volume and detection time, and high detection sensitivity Effect

Inactive Publication Date: 2011-09-21
SUZHOU HAOOUBO BIOPHARML
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

On the one hand, it can effectively retain the red blood cells in the whole blood sample, and solve the problem in the prior art that the red blood cells cannot be removed and the whole blood cannot be used as the sample for rapid diagnosis; on the other hand, the sealed water-absorbing medium is used as the Solid phase carrier, while effectively retaining red blood cells, basically does not cause the loss of other components in the sample

Method used

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  • Rapid immune detection method and detection kit for blood sample
  • Rapid immune detection method and detection kit for blood sample
  • Rapid immune detection method and detection kit for blood sample

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] In this embodiment, the above-mentioned detection kit was used to conduct a comparative test to test its function of retaining red blood cells.

[0025] Take three of the above-mentioned detection kits, respectively kits A, B, and C;

[0026] Kit A: The rabbit anti-human anti-erythrocyte IgG antibody is coated and fixed in the sample loading area (the coating method is to directly lyophilize the antibody and the non-water-absorbing medium in the sample loading area).

[0027] Kit B: Rabbit IgG antibody is coated and fixed in the sample loading area (the coating method is the same as kit A).

[0028] Kit C: No antibody is coated in the sample loading area.

[0029] Then add a drop of whole blood to each of the sample loading areas of the three kits (each kit adds the same whole blood, about 40 microliters). The experimental results are shown in Table 1. The results show that the rabbit anti-human anti-erythrocyte IgG can effectively retain the red blood cells in the sa...

Embodiment 2

[0033] The above detection kit and the rapid immunoassay method for blood samples of the present invention are used to detect the IgE content in the whole blood.

[0034] In the detection kit in this embodiment, the rabbit anti-human anti-erythrocyte IgG antibody is coated and fixed in the sample application area (the coating method is to directly freeze and freeze the antibody and the non-water-absorbing medium in the sample application area); Contains (unimmobilized form) mouse anti-human IgE monoclonal antibody coupled to blue beads; capture zone coated with immobilized goat anti-human IgE polyclonal antibody.

[0035] The samples were 5 parts of heparinized whole blood (background IgE 10IU / ml), to which 200, 100, 50, 20, 10 IU / ml of IgE were added respectively. Take 5 above-mentioned detection kits, and correspondingly, add 5 samples (about 40 microliters each) to the sample loading area of ​​each kit, and start the detection. The experimental results are shown in Table 2...

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Abstract

The invention relates to a rapid immune detection method and a detection kit for a blood sample. The method comprises the following step of: removing erythrocyte from the sample. The step of removing the erythrocyte is that: the whole blood sample is enabled to flow through a solid phase carrier which is coated and fixed with an anti erythrocyte antibody or an active section of the anti erythrocyte antibody; in the process that the sample flows through the solid phase carrier, the erythrocyte is combined with the anti erythrocyte antibody or the active section of the anti erythrocyte antibody, so that the erythrocyte is retained in the solid phase carrier; and the solid phase carrier consists of a water absorption medium which is subjected to sealing treatment. By the rapid immune detection method and the detection kit for the blood sample, the erythrocyte in the blood sample is effectively retained; and compared with the prior art, the invention has the advantages that: the detection can be directly performed by taking the whole blood as the sample, the limitation of a blood serum test method which only takes blood serum as a sample is overcome, blood volume and detection time are saved, and the detection sensitivity is high.

Description

technical field [0001] The invention belongs to the technical field of diagnostic immune analysis. Background technique [0002] Serological assay is one of the most important rapid medical diagnostic methods at present. The basic detection principle of the serological assay is to detect various indicators (such as the content of a certain immunoglobulin) in the patient's serum sample by using enzyme-linked immunosorbent assay. [0003] Currently, serological assays can complete the detection within a short period of time (10-30 minutes), achieving the purpose of rapid diagnosis. However, this method requires serum as a sample. Usually serum samples are prepared from whole blood, and only about 0.3 ml of experimental serum can be prepared from 1 ml of whole blood, and it takes 30 to 60 minutes. For special cases, for example, it is difficult to prepare sufficient serum samples in cases of limited blood collection (such as pediatrics), or in time-critical situations (such ...

Claims

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Application Information

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IPC IPC(8): G01N33/545G01N33/577
Inventor 李纪阳
Owner SUZHOU HAOOUBO BIOPHARML
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