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Test paper for quantitatively determining urea and preparation method of test paper

A technology for quantitative determination of urea, applied in biological testing, measuring devices, and material analysis through observation of the influence of chemical indicators, etc., can solve problems such as complex operations, and achieve simple structure of test paper, shortened detection time, and convenient operation Effect

Inactive Publication Date: 2020-11-24
湖北金鉴生物有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The enzyme coupling rate method can only detect serum and plasma samples and requires special equipment for detection, and the operation is relatively complicated

Method used

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  • Test paper for quantitatively determining urea and preparation method of test paper
  • Test paper for quantitatively determining urea and preparation method of test paper
  • Test paper for quantitatively determining urea and preparation method of test paper

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] The test paper for the quantitative determination of urea in this embodiment is composed of an upper plate, a diffusion layer, a blood filter layer, a reaction layer, a hydrophobic and gas-permeable color developing layer, and a lower plate, wherein:

[0037] The upper plate is made of ABS plastic, and the middle of the upper plate is provided with a sample hole;

[0038] The diffusion layer is made of commercially available hydrophilic gauze (selected from the commercially available Sati's hydrophilic series gauze), and cut the gauze into a size of 4mm*8mm for use;

[0039] The blood filter layer is made of glass fiber material (the commercially available PALL series glass fiber material is used) immersed in the blood filter solution, and the immersion treatment capacity is 495g / m 2 (That is, the glass fiber material is impregnated with 495g blood filtration solution per square meter), put it in a blast drying box and dried at 37℃, and the drying time is 12h. After drying, the...

Embodiment 2

[0065] The preparation of the test paper in this example, except that the composition of the blood filtration solution is 0.5% magnesium chloride, 150 mmol / L TAPS, 0.3 mg / mL hemagglutinin (PHA), and 1% polyvinylpyrrolidone, all other steps are the same. example 1.

Embodiment 3

[0067] The preparation of the test paper in this example, except that the composition of the reaction solution is 120mmol / L of CHES, 0.8% of Triton-100, 0.6% of zinc chloride, 150U / g of urease, 2% of sucrose, and 300U / g of ascorbic acid Except for oxidase, 2% sorbitol, 1% polyvinylpyrrolidone, and 0.5% casein, the other steps are the same as in Example 1.

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Abstract

The invention discloses test paper for quantitatively determining urea and a preparation method of the test paper, and belongs to the field of in-vitro diagnosis. The test paper comprises a diffusionlayer, a blood filtering layer, a reaction layer and a hydrophobic breathable color developing layer, wherein the blood filtering layer comprises buffer salt, divalent metal ions, polyvinylpyrrolidone, hemagglutinin or an anti-erythrocyte antibody, and the reaction layer comprises buffer salt, a surfactant, divalent metal ions, urease, polysaccharide, ascorbic acid oxidase, polyol, polyvinylpyrrolidone and an enzyme protective agent; the color developing layer contains an alcohol and a color developing agent. A blood filtering solution, a reaction solution and a color developing agent solutioncontaining related components are prepared, a blood filtering layer and a reaction layer material are soaked in the blood filtering solution and the reaction solution respectively, the color developing agent solution is sprayed on a hydrophobic breathable color developing layer material and dried to obtain a blood filtering layer, a reaction layer and a hydrophobic breathable color developing layer, and the blood filtering layer, the reaction layer and the hydrophobic breathable color developing layer are assembled with other components to obtain the test paper. The test paper provided by theinvention can simply, rapidly and quantitatively detect the content of urea in whole blood, plasma and serum.

Description

Technical field [0001] The invention belongs to the field of in vitro diagnosis, and relates to the detection of urea, in particular to a test paper for quantitative determination of urea and a preparation method thereof. Background technique [0002] Urea is the end product of amino acid catabolism in the body. Hepatocytes can make ammonia produce urea, and the liver is the most important organ for producing urea. Urea is the main component of non-protein nitrogen in the blood, and normally accounts for about 50% of the total non-protein nitrogen. Urea is mainly excreted by the kidneys. All urea in the blood is filtered by the glomerulus, and 30%-40% is reabsorbed by the renal tubules. The renal tubules also secrete a small amount of urea, which increases in severe renal failure. The determination of urea is one of the commonly used indexes to measure glomerular function. [0003] At present, the most commonly used methods for determining urea in clinical laboratories are the d...

Claims

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Application Information

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IPC IPC(8): G01N33/62G01N33/52G01N21/78
CPCG01N33/62G01N33/526G01N21/78
Inventor 熊攀李强
Owner 湖北金鉴生物有限公司
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