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Method and reagent card for whole blood quick determination of homocysteine

A technology for rapid determination of homocysteine, applied in the field of rapid determination of homocysteine ​​in whole blood, can solve the problem of inability to achieve efficient and simple homocysteine ​​testing, hemolysis or red blood cell removal is not clean, inconvenient and instant Detection and other problems, to achieve the effect of not easy hemolysis, good removal effect, and simplified operation

Inactive Publication Date: 2016-02-03
ANHUI DAQIAN BIO ENG LIMITED
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the methods for determining homocysteine ​​in the human body mainly include: chemiluminescence method, cystathionine method, hydrolytic enzyme method, liquid fluorescence method, etc. All of the above methods require pre-separation of red blood cells from whole blood before testing. A special separation device, because the red blood cells in whole blood contain a large amount of homocysteine, which has a great impact on the test results. Hemolysis or unclean removal of red blood cells will affect the measurement results
Moreover, all the above-mentioned methods are liquid-phase reaction systems, which require special equipment for reaction.
Therefore, none of the above-mentioned methods can achieve efficient and simple testing of homocysteine, and it is even more inconvenient to perform instant testing outdoors and in remote areas.

Method used

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  • Method and reagent card for whole blood quick determination of homocysteine
  • Method and reagent card for whole blood quick determination of homocysteine
  • Method and reagent card for whole blood quick determination of homocysteine

Examples

Experimental program
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Effect test

Embodiment 1

[0037] see figure 1 , the reagent card for rapid determination of homocysteine ​​in whole blood in this embodiment includes a filter layer 1, an adsorption layer 2, a reagent layer 3 and a color development layer 4 from top to bottom, the filter layer 1 and the adsorption layer 2 Made of cellulose membrane, the adsorption layer 2 contains anti-erythrocyte antibody, the reagent layer 3 is made of nitrocellulose membrane (NC), and the reagent layer 3 contains reducing agent 2-mercaptoethanol and homosemi Cystine lyase, the color-developing layer 4 is made of qualitative filter paper, and the color-developing layer 4 contains color-developing agents ferric chloride and N,N'-di-n-butyl-p-phenylenediamine;

[0038] The thicknesses of filter layer 1, adsorption layer 2, reagent layer 3 and chromogenic layer 4 are 200 μm, 370 μm, 250 μm and 120 μm respectively, and the dosage of 2-mercaptoethanol is 0.5 g / dm 2 , the dosage of homocysteine ​​lyase is 5KU / dm 2 , the amount of ferric ...

Embodiment 2

[0041] see figure 1 , the reagent card for rapid determination of homocysteine ​​in whole blood in this embodiment includes a filter layer 1, an adsorption layer 2, a reagent layer 3 and a color development layer 4 from top to bottom, the filter layer 1 and the adsorption layer 2 Made of cellulose membrane, the adsorption layer 2 contains anti-erythrocyte antibody, the reagent layer 3 is made of nitrocellulose membrane (NC), and the reagent layer 3 contains reducing agent 2-mercaptoethanol and homosemi Cystine lyase, the color-developing layer 4 is made of qualitative filter paper, and the color-developing layer 4 contains color-developing agents ferric chloride and N,N'-di-n-butyl-p-phenylenediamine;

[0042] The thicknesses of filter layer 1, adsorption layer 2, reagent layer 3 and chromogenic layer 4 are 120 μm, 250 μm, 120 μm and 370 μm respectively, and the dosage of 2-mercaptoethanol is 0.1 g / dm 2 , the dosage of homocysteine ​​lyase is 1KU / dm 2 , the amount of ferric ...

Embodiment 3

[0045] see figure 1 , the reagent card for rapid determination of homocysteine ​​in whole blood in this embodiment includes a filter layer 1, an adsorption layer 2, a reagent layer 3 and a color development layer 4 from top to bottom, the filter layer 1 and the adsorption layer 2 Made of cellulose membrane, the adsorption layer 2 contains anti-erythrocyte antibody, the reagent layer 3 is made of nitrocellulose membrane (NC), and the reagent layer 3 contains reducing agent 2-mercaptoethanol and homosemi Cystine lyase, the color-developing layer 4 is made of qualitative filter paper, and the color-developing layer 4 contains color-developing agents ferric chloride and N,N'-di-n-butyl-p-phenylenediamine;

[0046] The thicknesses of filter layer 1, adsorption layer 2, reagent layer 3, and chromogenic layer 4 are 370 μm, 120 μm, 370 μm, and 200 μm, respectively, and the dosage of 2-mercaptoethanol is 1 g / dm 2 , the dosage of homocysteine ​​lyase is 10KU / dm 2 , the amount of ferri...

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Abstract

The invention belongs to a method for whole blood quick determination of homocysteine. The method comprises the following steps: (1) filtering whole blood by a filter material to filter red cells in the whole blood; (2) performing absorption processing on the filtered whole blood by anti-erythrocyte antibodies to adsorb unfiltered red cells; (3) reducing homocysteine in the whole blood subjected to the absorption processing by a reducing agent into reduced homocysteine, and then performing pyrolysis on the reduced homocysteine by homocysteine lyase to generate H2S; (4) performing a fluorescence reaction on the H2S and a color developing agent and finally reading a result. The method needs no special separation device, can remove the red cells in the whole blood by the filter material and the anti-erythrocyte antibodies, and is good in removing effect, thus ensuring the testing accuracy and greatly simplifying the operation.

Description

technical field [0001] The invention relates to the technical field of medical testing and determination, in particular to a method for rapidly measuring homocysteine ​​in whole blood and a reagent card. Background technique [0002] Homocysteine ​​(HCY) is a sulfur-containing amino acid produced by the metabolism of methionine. 80% of HCY binds to proteins through disulfide bonds in the blood, and only a small part of free homocysteine ​​participates in the circulation. Homocysteine ​​has been widely accepted as an independent cardiovascular risk indicator, and it is another risk factor besides hyperlipidemia, smoking, and diabetes. Although much remains to be clarified about the biochemical basis of hyperhomocysteinemia, the relationship of its detection to various diseases and the detection of treatment will require further investigation. [0003] At present, the methods for determining homocysteine ​​in the human body mainly include: chemiluminescence method, cystathion...

Claims

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Application Information

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IPC IPC(8): G01N21/64G01N1/28
Inventor 芮双印
Owner ANHUI DAQIAN BIO ENG LIMITED
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