97results about How to "Many binding sites" patented technology

The invention belongs to the field of water treatment, and particularly relates to a magnetic covalent bond type chitosan-based modified flocculant and a preparation method and application thereof. According to the invention, a cationic monomer and a hydrophobic monomer are introduced into chitosan molecules through a graft copolymerization reaction, so that the flocculant is ensured to have good stability, the electricity neutralization capability and the demulsification capability on oil droplets of the flocculant in a flocculation process are improved, the solubility of the flocculant is increased, the pH application range of the flocculant is widened, the dosage is reduced, meanwhile, the nano Fe3O4 has superparamagnetism, the settling performance of floc can be remarkably improved under the action of an external magnetic field when the nano Fe3O4 is introduced into the flocculating agent, and the settling time is shortened. Results of the embodiment show that the flocculant provided by the invention is good in lipophilicity and excellent in flocculation performance, and has efficient oil-water separation capacity, wider pH application range and excellent recoverability.

The invention discloses a high-solid content polyamide polyamine epichlorohydrin wet strength agent, as well as a preparation method and an application thereof. The preparation method comprises the following steps: performing condensation on a dibasic acid and a diamine under the action of an acidic catalyst to get a polyamide polyamine intermediate; enabling the polyamide polyamine intermediate, an organic chloride and a cation active monomer or a mixture of the cation active monomer and sodium hydroxide to perform nucleophilic addition reaction to get a high-viscosity cation polymer; and further adding water-soluble hydroxy amine to react and regulating pH with an acid to get the high-solid content polyamide polyamine epichlorohydrin wet strength agent. The obtained wet strength agent has the advantages of high solid content above 40% (in percentage by weight), high charge density, stability in storage, great wet-strengthening performance and few chlorine-containing byproducts, and can be applied in papermaking wet-end chemistry process.

The invention discloses a negative pole of an enzymatic biofuel cell as well as a preparation method and application of the negative pole. The negative pole is prepared by taking carbon paper as an electrode substrate, an enzyme as a catalyst and a carbon nano-tube / cibacron blue composite as an enzyme carrier, wherein the carbon nano-tube / cibacron blue composite is a carbon nano-tube modified by cibacron blue. The negative pole of the enzymatic biofuel cell disclosed by the invention has rich surface active groups which provide rich bonding sites for the load of enzyme, and has favorable conductivity; an enzymatic biofuel cell electrode prepared by the negative pole of the enzymatic biofuel cell can be used for greatly increasing the power density of the enzymatic biofuel cell.

The invention discloses a manufacturing method of a drug vessel support with antibody immobilized on the surface of the support. The manufacturing method comprises the following steps: 1) preparing polyester high polymer with carboxyl functional groups by using a synthesis or hydrolysis mode; 2) coating drug loading polyester high polymer on the surface of the support; and 3) immobilizing the antibody with the function of capturing endothelial progenitor cells on the surface of the support body by using a chemical coupling mode. In respect of the drug vessel support with antibody immobilized on the surface of the support, polyester high polymer with carboxyl functional groups is taken as the support drug loading coating to obtain the drug support the surface of which is subject to carboxylation, so that the surface of the support has both biocompatible compatibility and biological activity, and the carboxyl functionalized surface of the support provides abundant binding sites for loading effective dosage of antibody in the next step. Meanwhile, the support has a drug carrying function and an endothelial cell capturing function, thereby playing excellent roles in treating restenosis and promoting endothelial cell repairing.

The invention discloses a method for preparation of triazole molecularly imprinted electrochemical sensor based on an electropolymerized OPD / OAP composite membrane. The method includes glassy carbon electrode pretreatment, electropolymerization of OPD / OAP composite membrane, template molecule elution and other steps in order. According to the invention, a triazole substance is taken as the template molecule, OPD and OAP are adopted as the mixed functional monomer, the use of the composite functional monomer improves the selection performance of the binding site in a polymer film, and is conducive to forming a stable host-guest compound from a template molecule and a functional monomer, so that the template molecule can maintain a good rigid structure in a polymer network structure and form a cavity with memory effect. The preparation method is simple and easy to control, and the sensor has the advantages of strong selectivity, good stability, high sensitivity, low cost, and fast response. The invention also provides application of the sensor in adsorption of triazole substances. The sensor prepared by the method provided by the invention is suitable for analysis and detection of residual triazole pesticide in vegetables, fruits and other agricultural products.

The invention discloses a method for preparing sulfydryl modified ferroferric oxide / silicon dioxide magnetic nanoparticles. The method comprises the following steps that 1, magnetic ferroferric oxide particles are prepared with trivalent ferric salt serving as raw materials through a solvothermal method; 2, tetraethoxysilane is adopted as a silicon source, generated silicon dioxide wraps the surfaces of the magnetic ferroferric oxide particles through a sol-gel method, and ferroferric oxide / silicon dioxide magnetic nanoparticles are formed; 3, the ferroferric oxide / silicon dioxide magnetic nanoparticles are placed in a methylbenzene solution and dispersed evenly to obtain a solution A, dimercaptosuccinate is added to a dimethyl sulfoxide solution and dispersed evenly to obtain a solution B, the solution A and the solution B are mixed and stirred and react for a period of time, then solid-liquid separation, cleaning and drying are carried out, and then the sulfydryl modified ferroferric oxide / silicon dioxide magnetic nanoparticles are obtained. The magnetic nanoparticles are good in dispersing performance, achieve good stability and biocompatibility, and has potential application value in the bio-medical field.

The invention discloses a dual-response type virus molecularly imprinted sensor based on magnetism and photons, and a construction method thereof. The dual-response type virus molecularly imprinted sensor based on magnetism and photons takes magnetic particles as an inner core, the surfaces of the magnetic particles are coated with silicon dioxide, a metal organic framework is grafted to the silicon dioxide, the metal organic framework is connected with a photoresponse polymer, and the photoresponse polymer is connected with a target virus template. The dual-response type virus molecular imprinting sensor based on magnetism and photons has a good binding behavior on a template virus, and is fast in binding kinetics, high in adsorption capacity and good in selectivity.

The invention belongs to the technical field of biological detection and discloses a method for detecting pathogens with sandwich structure through SERS. The method comprises the following steps thatincubation is performed on antimicrobial peptide functionalized magnetic nanoparticles and bacteria, magnetic nanoparticles which adsorb the bacteria are separated out under the effect of an externalmagnetic field to obtain a magnetic nanoparticle-bacterium compound, then SERS probe co-incubation is performed on a mercaptophenylboric acid modified graphene oxide-silver-coated gold nano-compound to form a sandwich structure formed by antimicrobial peptide functionalized magnetic nanoparticle-bacterium-SERS probe, the sandwich structure is separated out under the effect of the external magneticfield, and detection and identification are performed on different bacteria through SERS.

The invention belongs to the technical field of SERS substrates and relates to a silver nanoflower composite graphene SERS substrate, a preparation method and application thereof. According to the structure of the SERS substrate, two electric conduction electrodes are arranged at two ends of one surface of a substrate respectively; a groove is formed between the two electric conduction electrodesand the substrate; a first graphene layer, a first silver nanoflower layer, a second graphene layer and a second silver nanoflower layer are sequentially distributed in the groove from bottom to top;the first silver nanoflower layer and the second silver nanoflower layer are respectively grown on the first graphene layer and the second graphene layer; and the second graphene layer is grown on thefirst silver nanoflower layer. According to the SERS substrate of the invention, the graphene formed between the two silver nanoflower layers forms a coupled electromagnetic enhanced region, so thatthe hot spot density of the surface of the material can be remarkably increased; and the silver nano-structure at the bottom enables the graphene coating the surface of the silver nano-structure to form a fluctuating fold-shaped form, and therefore, the specific surface area of the surface of the material is increased, and more binding sites are provided for molecules to be detected.

The invention discloses a supported single-layer and few-layer two-dimensional transition metal sulfide catalyst and a preparation method thereof. According to the method, water is used as a solvent and mixed with a carrier, a soluble metal precursor and glutathione, and the supported single-layer and few-layer two-dimensional transition metal sulfide catalyst is prepared through one-step hydrothermal synthesis at the temperature of 160-240 DEG C. The process is easy to implement; and the prepared catalyst is small in single-layer and few-layer two-dimensional transition metal sulfide size andgood in dispersity, and the average stacking layer number is 1-10. The obtained catalyst has application prospects in the aspects of hydrogenation, hydrogen production, electrochemical sensing, secondary batteries and the like.

The invention relates to the field of SPR sensor chips, and particularly relates to a ractopamine molecularly imprinted SPR sensor chip as well as a preparation method thereof. A ractopamine molecularly imprinted polymer, graphene nanogold, and 1-octyl-3- methylimidazole hexafluorophosphate are taken as chip substrate modifiers for the ractopamine molecularly imprinted SPR sensor chip. The ractopamine molecularly imprinted SPR sensor chip provided by the invention has high-specificity adsorption capability to ractopamine, and has obvious response signal variation to low-concentration ractopamine in samples. The ractopamine molecularly imprinted SPR chip prepared by the invention is simple in steps, is short in analysis time, and has relatively high accuracy and flexibility when being used for analyzing the low-concentration ractopamine residue.

The invention belongs to the technical field of a magnetic molecular imprinting polymer, and particularly relates to a magnetic molecular imprinting polymer for separating and enriching perfluorooctane sulfonate and a preparation method thereof. The magnetic molecular imprinting polymer has an ultrathin molecular imprinting film, and achieves fast adsorption dynamics, high adsorption selectivity and repeated use performance; the perfluorooctane sulfonate in water can be effectively and fast removed.

The invention relates to a compound flocculant with polymerization ferric chloride and amination polymers based on papermaking sludge and a method for preparing the compound flocculant. The method includes synthesizing amination polymers from the papermaking sludge generated in the papermaking industry; compounding the amination polymers and the inorganic flocculant polymerization ferric chloride to prepare the compound flocculant with the polymerization ferric chloride and the amination polymers based on the papermaking sludge. The compound flocculant and the method have the advantages that the color removal rate of disperse yellow water samples can reach 95% at least when the compound flocculant is used for treating the simulated dye water samples and the dosing quantity of the compound flocculant is 5 mg / L, excellent flocculation effects can be realized by the compound flocculant for water samples under different acidity and alkalinity conditions and other diversified water samples, the capital construction cost of reaction tanks and sedimentation tanks can be reduced, and the compound flocculant is high in electricity performance and flocculation efficiency, good in dissolving property, long in storage cycle and applicable to the field of water supply, wastewater treatment and the like and suitable for actual application to water treatment.

The invention discloses a soil restoration agent for restoring heavy metal polluted soil and a preparation method thereof. The soil restoration agent is prepared from turfy soil, brown coal, porous modified biocarbon, composite bacterium powder, and medical stone. The preparation method comprises following steps: (1) weighing all raw materials according to a formula; (2) adding brown coal and medical stone into a pulverizer, and grinding the brown coal and medical stone; (3) adding all raw materials into a high speed mixing machine, stirring, and evenly mixing; and (4) carrying out wet granulation to convert the mixture into granules, namely the soil restoration agent. The raw materials are easily available, the preparation technology is scientific, and the soil restoration agent can restore soil polluted by various heavy metals, is capable of improving the quality and structure of soil and increasing the nutrients of soil, and creates great social benefits, economic benefits, and environmental benefits.

The invention relates to a preparation method of a graphene oxide / cellulose composite biological supported biodiesel catalyst. The graphene oxide / cellulose composite biobased supported catalyst is made by adopting microwave-assisted ionic liquid dissolution cellulose technology, high-speed homogeneous ultrasonic-assisted peeling technology, particle-size-controllable sphere preparation technology, composite microsphere amino modification technology and supporting-amount-controllable composite microsphere supporting technology. Through adjustment of technical parameters, catalysts with different particle sizes, amino grafting types and heteropoly acid supporting amounts are obtained and can be used for adapting to different base oil types, reactant consumption and reaction environments, thereby achieving the maximum biodiesel yield. Moreover, the prepared composite microsphere catalyst selects renewable raw materials, is low in price, and is green and eco-friendly. The catalyst is excellent in catalytic activity and mechanical property, high in recycling frequency, and convenient to recycle after reaction, and is an ideal heterogeneous catalyst in biodiesel production.

The invention aims to provide a preparation method of a lithium ion battery negative electrode material. According to the technical scheme, the method comprises the following steps: mixing proper amount of 2,2-bis[4-(4-aminophenoxy)phenyl]hexafluoropropane, 3,3,4,4-biphenyl tetracarboxylic dianhydride and graphene oxide to prepare PAA / GO, further adding acetic anhydride and triethylamine to prepare a concentrated solution, carrying out high-temperature sintering to obtain a PI-rGO composite material, then adding a proper amount of N-methyl pyrrolidone, polyimide, polyvinylidene fluoride, nanosilicon particles, carbon nanotubes and beta-cyclodextrin to obtain conductive slurry, further drying and cooling, and slicing on a manual slicing machine to obtain the required lithium ion battery negative electrode material. In the use process of the negative electrode material, the probability of side reaction is low, silicon particles attached to the surface of the negative electrode materialare not prone to falling off in the charging and discharging process, sudden reduction of capacity is prevented, and the negative electrode material has good lithium ion conduction performance.

The invention relates to an anti-shearing reinforcement device and method for a concrete box girder web. In order to solve the problem that in existing engineering technologies, the concrete box girder web has cracking inclined cracks and insufficient anti-shearing bearing capacity, an external vertical prestressing tendon reinforcement device and method are provided. The device comprises groove-shaped steel, L-shaped steel, vertical prestressing tendons, wedge-shaped steel cushion blocks and an anti-corrosion coating at the outer side of the web. The groove-shaped steel is horizontally fixedto the junction of the upper end of the web and a top plate in the girder length direction. The L-shaped steel (6) is horizontally fixed to the lower end of the web in the girder length direction andis parallel to the groove-shaped steel (5). The vertical prestressing tendons are vertically mounted on the groove-shaped steel and the L-shaped steel, and it is ensured that the vertical prestressingtendons are parallel to the plane of the web. According to the anti-shearing reinforcement device and method, the web is connected with the top plate and a bottom plate through finish rolling twistedsteel, the force transmission path is definite, the risk that web concrete is pulled broken is reduced, and meanwhile, midspan deformation is reduced; the problem of insufficient main tensile stressof the web concrete is effectively solved, and the situations of continuous extension of the inclined cracks of the web or new inclined cracks are prevented.

The invention belongs to the technical field of preparation of electrochemical electrode materials, and provides an electrochemical sensor for detecting glucose and a preparation method thereof. The electrochemical sensor is an electrochemical sensor based on cobalt@nickel-metal organic framework-graphene (CNMs-GR), and the sensor is used for simultaneously detecting glucose in human serum. The sensor shows high sensitivity and selectivity, the electrode modification process is simpler and more convenient, and good stability and reproducibility are achieved. Based on a cobalt@nickel-metal organic framework-graphene (CNMs-GR) nano composite material, a bimetallic framework is synthesized through a simple hydrothermal reaction, and the cobalt@nickel-metal organic framework-graphene CNMs-GR nano composite material is prepared. The sensitivity of the electrode is improved; and the electrode modification process is simpler. The electrochemical sensor is used for constructing a sensing system for detecting glucose in human serum, and the selectivity of the electrode is remarkably improved. The detection limit is 0.087 [mu]mol / L. The linear range is wide and the sensitivity is high.

The invention belongs to the field of immunological detection and particularly relates to a method for evaluating the blocking activity of an immune checkpoint molecular blocking antibody by magneticbeads. Magnetic beads with solid-phase anti-tag molecular antibodies and immune checkpoint molecular protein expressing label molecules (label I) subjected to solid-phase on the surfaces of the magnetic beads are used; the magnetic beads can be specifically bound with a ligand of an immune checkpoint molecule with another label molecule (label II), and then a label II specific binding antibody labeled by a biologic substance (fluorescent or visible light) is used for quantitatively detecting the immune checkpoint molecular protein / ligand molecular protein compound on the surface of the magnetic bead. When the blocking antibody of the immune checkpoint molecule, the magnetic beads with the immune checkpoint molecule protein in the solid phase and the ligand protein coexist, the blocking activity of the antibody is inversely proportional to the final luminous intensity of the magnetic beads, so that quantitative analysis on the blocking activity of the antibody is realized through lightintensity detection. The magnetic bead method is simple to operate, quick in reaction, economical and time-saving.

The invention discloses a molecule imprinted fluorescent sensor for detecting viruses. A metal organic framework is used as a carrier material of the sensor, a metal chelator is used as a functional monomer, and a six-membered ring is formed between a template molecule and the functional monomer through metal chelation, so as to prepare the sensor. Specific identification capability of the sensorfor template viruses is improved through deactivation. The metal organic framework has high specific surface area, and can provide more imprinting sites, improving response sensitivity of the sensor.Deactivation lowers non-specific adsorption, so that selectivity of the sensor for the template molecule is improved; metal coordination reduces interference of covalent force to template elution andfixes template molecule more effectively relative to non-covalent force; the fluorescent analysis method has advantages of high flexibility, high selectivity, convenience in operation, and so on. Compared with other analysis methods or sensors, the molecule imprinted fluorescent sensor prepared in the invention has high selectivity for template molecules and high detection flexibility, and has high practical application value and significance in biosensing and virus detection and prevention.

The invention discloses a carrier material of immobilized papain and a preparation method of the carrier material. The method comprises the steps as follows: liquid paraffin and trichloromethane are mixed, then an emulsifier is added, the mixture is uniformly stirred and kept at the temperature of 60-70 DEGC, a mixed solution is added to the mixture with stirring, the mixture is continuously stirred for 20-30 min after addition and cooled to the room temperature, a supernatant is removed through centrifugation, prepared microspheres are washed three times with petroleum ether and then washed with water, and the carrier material of the immobilized papain is prepared. The invention further discloses the immobilized papain from the carrier material and a preparation method of the immobilized papain. The carrier material can be firmly combined with the papain, and the papain cannot fall off easily during usage. Besides, the papain can still keep higher activity after being immobilized on the carrier.

The invention discloses a boron hydride anion supramolecular organic frame material. The material is obtained by forming a two-dimensional plane structure by complexing a metal ion M and an organic nitrogen-containing ligand L and then connecting cage-like boron hydride anions [B12H12]2- or [B10H10]2- through various supramolecular interaction of negative hydrogen-normal hydrogen dihydrogen bond action, negative hydrogen-metal action and the like, wherein the metal ion M is selected from at least one of Zn, Cu, Ni and Co, and the organic nitrogen-containing ligand L is selected from at least one of pyrazine, dipyridyl, dipyridyl acetylene, dipyridyl ethylene, dipyridyl benzene and azobispyridine. The boron hydride anion supramolecular organic frame material can be used for high-selectivityadsorption and separation of propane / ethane / methane, carbon dioxide / methane and acetylene / ethylene.

The invention discloses a flexible conductive fiber membrane material and a preparation method thereof. The preparation method comprises steps as follows: a fiber base material, a conductive nano material and a surfactant are dissolved in an organic solvent, and a spinning solution is prepared; a nano fiber membrane is prepared from the spinning solution with an electrospinning process; a conductive polymer is polymerized on the nano fiber membrane in situ, and the flexible conductive fiber membrane material is prepared, wherein the nano fiber membrane has a structure of mutually connected primary main fiber and secondary cobweb fiber, and the conductive polymer is polymerized in situ and formed on the primary main fiber and the secondary cobweb fiber. In the presence of the cobweb fiber,the specific surface area of fiber in samples is increased, so that during in-situ polymerization reaction, more binding sites can be provided for attachment of the conductive polymer, and the conductive performance of the conductive fiber membrane material is substantially improved.

The invention discloses a preparation method of a modified lithium ion battery diaphragm, and belongs to the technical field of modification of lithium ion battery diaphragms. The preparation method comprises the following steps: preparing an ethanol solution of Zn (AC) 2.2 H2O and CTAB (cetyltrimethyl ammonium bromide), mixing to form a faint yellow precipitate, adding nano-diamond, heating to obtain a nano-diamond / zinc oxide composite material ZnO / NDs, preparing an NDs colloidal solution or a ZnO / NDs colloidal solution, coating a polypropylene diaphragm with the solution to obtain the modified lithium ion battery diaphragm material, and the like. The novel diaphragm modification material prepared by the invention has good electrochemical performance, and the lithium ion battery prepared from the novel diaphragm modification material has good cycle stability and incremental capacity.

The invention relates to the technical field of biotechnology, in particular to a quantitative detection method of creatine kinase isoenzyme MB. The method comprises preparation of a creatine kinase isoenzyme MB solution, a microfluidic creatine kinase isoenzyme MB detection chip and a use method of the microfluidic creatine kinase isoenzyme MB detection chip. A micro-fluidic chip is structurallycharacterized by comprising a micro-pipeline network. Each working unit can independently complete single reaction or unit operation, and can also cooperate with other units to complete complex work,so that the whole chip is called a dynamic reaction system. The method has the main characteristics of high flux, miniaturization and automation, and is an innovation and leap in the aspects of traditional biotechnology, disease diagnosis and the like; immunomicrosphere chemiluminescence is combined with a micro-fluidic chip, so that a full-automatic instrument is more miniaturized and simplified,and later pipeline maintenance and mechanical arm maintenance are reduced; and the combination of the two converts the original POCT application solid-phase reaction into liquid-phase homogeneous reaction, and the method has the advantages of high reaction efficiency, high sensitivity and detection interval of the traditional tubular chemiluminiscence.

The invention provides a preparation method of an SERS substrate of a Ti / TiOx / Ag hybrid composite material. The preparation method comprises the following steps of: firstly, preparing a Ti / TiOx nano-film from a titanium foil and a NaOH solution through a hydrothermal synthesis reaction at 200 DEG C for 6 hours; immersing the Ti / TiOx film into an AgNO3 solution and carrying out redox reaction for 2hours under the irradiation of an ultraviolet lamp of a three-purpose ultraviolet analyzer with the wavelength of 365nm, so as to enable nano-silver to modify in the interior of the Ti / TiOx nano-film, thereby obtaining the SERS substrate of the Ti / TiOx / Ag hybrid composite material. The SERS substrate of the Ti / TiOx / Ag hybrid composite material prepared by the invention is simple in preparation method and low in equipment requirement, and has relatively good sensitivity and relatively good uniformity and stability in real object detection.

The invention relates to a novel synthesis acrylic acid emulsion for spinning and a preparation method thereof. The emulsion is prepared from, by weight, 50-60 parts of a soft monomer, 20-30 parts ofa hard monomer, 10-30 parts of a functional monomer, 3-8 parts of an emulsifier, 0.3-1 part of an initiator, 0.5-2 parts of organic modified nanometer layered bimetal hydroxide, 1-8 parts of a pH regulator and 100-200 parts of deionized water. Compared with the prior art, by adding MgAl-SDS-LDH, more bonding loci exist between the sheet inorganic additive and polymer molecules, the more dense structure is formed, and the tenacity of the film is improved; due to the mutual effect between the LDH sheet layer and the polymer molecules, the system moisture absorption and re-stickiness are lowered,and the technology is simple and suitable for industrial production.

The invention relates to the technical field of catalysts, and discloses a Ni-NiO / C core-shell structure nanomaterial electrocatalyst and a preparation method thereof so as to solve the problem that an existing electrocatalyst is low in catalytic efficiency of catalytic hydrogen evolution; the preparation method comprises the following steps: (1) dissolving nickel chloride hexahydrate in distilled water to form a first solution; (2) adding aminoacetic acid and isopropanol into the first solution to form a second solution; (3) stirring the second solution to form turbid liquid, transferring the turbid liquid into a container, carrying out gradient heating reaction, and cooling to room temperature to obtain light green precipitate; (4) washing the collected light green precipitate cleanly, and performing vacuum drying to obtain a precursor; (5) preliminarily calcining the precursor in inert gas to obtain a Ni / C compound; and (6) carrying out secondary calcination on the Ni / C compound in air. The synthesis method provided by the invention has the characteristics of simple process, low energy consumption, mild conditions, good product morphology and the like, and is suitable for large-scale production and application.

The invention relates to the technical field of building crack repairing, in particular to an outer wall construction joint leakage repairing technology. The outer wall construction joint leakage repairing technology comprises the following construction steps that S1, an outer wall construction joint facing layer is shoveled; s2, embedding a grouting needle head; s3, slurry preparation and grouting; and S4, the grouting needle head is removed, and the grouting hole is blocked. By means of the construction method and the obtained leaking stoppage grouting material, the anti-permeability of the leaking stoppage grouting material is remarkably improved, repeated cracking caused by damp or other reasons is not likely to happen, and then the repairing effect of a wall is guaranteed.

The invention discloses a nickel oxide electrochromic composite film and a preparation method and application thereof. The nickel oxide electrochromic composite film comprises a nickel oxide layer and an amorphous zinc tin oxide buffer layer which are arranged in a laminated mode. The nickel oxide layer is provided with a crystal nano columnar structure, and the diameter of the crystal nano columnar structure is 10 to 100 nm. The charge capacity of the nickel oxide electrochromic composite film is 6.0 to 12.0 mC *cm<-2>. The nickel oxide electrochromic composite film prepared by the preparation method disclosed by the invention has better optical modulation amplitude, cycling stability and higher charge capacity, and has a good application prospect in a low-temperature coated flexible electrochromic device.