The application provides a
nucleic acid test strip and a detection method for detecting
smut genes, and belongs to the technical field of detection. The application comprises a bottom plate, a sample pad and a water absorption pad; an NC film is attached to the bottom plate, the NC film is provided with a T line and a C line, the T line is sprayed with a
coupling agent of a capture probe and
streptavidin; the C line is sprayed with a
coupling agent of a
quality control probe and
streptavidin; the
quality control probe is complementary to
a DNA sequence of
a DNA1-AgInS2 / ZnS fluorescent probe. The application utilizes the advantages of AgInS2 / ZnS
quantum dots (AgInS2 / ZnS QDs), such as good
biocompatibility, high
fluorescence intensity and wide spectral range. The test strip based on AgInS2 / ZnS
quantum dots has higher sensitivity and stronger quantitative capacity than traditional
test strips. The
fluorescence ratio of the T line and the C line is detected by using a
fluorescence immunoassay instrument for quantitative detection, the
fluorescence immunoassay instrument can quickly read out the value, and has the advantages of rapidness, simple operation, low sensitivity, good selectivity and the like.