Applications of SPA-antibody trimer as indicator in detection of blood antigens

A trimer and antibody technology, applied in the field of biomedicine, can solve the problems of high cost, high technical requirements, and difficulty in clinical application and application, and achieve the effects of low cost, easy promotion and simple method.

Inactive Publication Date: 2014-03-05
王信
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The common problems of these methods are: firstly, all types of stem cells cannot be effectively preserved; secondly, the cost is expensive and the technical requirements are relatively high
There are certain difficulties in clinical application

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Example 1: Cell Treatment Kit

[0045] 1. Trimer of SPA (staphylococcal protein A), red blood cell antibody and specific lymphocyte antibody. The preparation process of the trimer: the reaction concentrations of SPA and anti-erythrocyte monoclonal antibody and anti-cd3 antibody, cd14 antibody, cd19 antibody and cd56 antibody are all 0.1 mg / ml. SPA is 1.5ml, anti-erythrocyte monoclonal antibody is 1ml, anti-cd3 antibody, cd14 antibody, cd19 antibody, and cd56 antibody are each 0.25ml. Incubate in a 37°C water bath for 30 minutes. Add PBS solution, adjust the concentration of Spa-antibody trimer to 1 μg / ml, volume to 100ml, pH 7.4. Store at 4°C.

[0046] 2. Human lymphocyte separation medium (commercially available) 100ml, centrifugal density 1.077g / ml.

[0047] 3. Cell chromatography plate: polystyrene chromatography plate, after disinfection and sterilization, add anti-cd3 antibody, cd14 antibody, cd19 antibody, and cd56 antibody at a concentration of 0.1mg / ml, just ...

Embodiment 2

[0051] Example 2: Cell Treatment Kit

[0052] 1. Trimer of SPA (staphylococcal protein A), red blood cell antibody and specific lymphocyte antibody. The preparation process of the trimer: the reaction concentrations of SPA and anti-erythrocyte monoclonal antibody and anti-cd3 antibody, cd14 antibody, cd19 antibody and cd56 antibody are all 0.1 mg / ml. SPA is 1.5ml, anti-erythrocyte monoclonal antibody is 1ml, anti-CD3 antibody, CD14 antibody, CD19 antibody, CD56 antibody is 0.25ml. Incubate in a 37°C water bath for 30 minutes. Add PBS to make the Spa-antibody trimer concentration 1 μg / ml, volume 100ml, pH 7.4. Store at 4°C.

[0053] 2. Lymphocyte separation solution 100ml: an aqueous solution composed of 0.3% sodium diatrizoate and 8% polysucrose 400#, with a density of 1.077g / ml.

[0054] 3. Antibody conjugate: mix anti-CD3 antibody, CD14 antibody, CD19 antibody, CD56 antibody and nanoparticles of polystyrene material. The antibody concentration is 0.1 mg / ml, the volume is...

Embodiment 3

[0058] Example 3: Spa-antibody

[0059] Chicken Marek's disease (MD) is a highly contagious lymphoproliferative disease caused by Marek's virus. Its main features are: lymphocyte infiltration and swelling of peripheral nerves, various organs, iris and skin. Marek's disease mainly occurs in chickens, and has also been reported in turkeys, pheasants, ducks, quails, swans and other poultry. The disease is highly contagious, spreads quickly, and has a long incubation period. Acute onset chickens are eliminated and the mortality rate reaches 10% to 80%. So far, in the diagnosis of Marek's disease, except for AGPT, there is no practical technique that can be easily applied at the grassroots level.

[0060] Trimer of SPA (staphylococcal protein A) and erythrocyte antibody, anti-Marek's virus antibody. The preparation process of the trimer: the reaction concentrations of SPA, anti-erythrocyte monoclonal antibody and anti-Marek's virus antibody are all 0.1 mg / ml. SPA is 1.5ml, ant...

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PUM

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Abstract

The invention relates to applications of an SPA-antibody trimer as an indicator in detection of blood antigens. The trimer is formed of anti erythrocyte antibodies, SPA and some kind of anti leucocyte or other antigen antibodies. The self anti erythrocyte antibodies are combined with erythrocytes, another kind of antibodies are combined with corresponding leucocyte antigens (or other antigens), then leucocytes (or other cells and factors) and erythrocytes are sedimentated through a blood sedimentation process or other methods such as density gradient centrifugation and the like, so the trimer has the effect of removing components of corresponding cells and the like. The trimer also can combine erythrocytes and corresponding antigens by means of SPA and is used for detecting certain antigens.

Description

[0001] The present invention is a divisional application for an invention patent titled "SPA-antibody trimer, a cell treatment kit containing the trimer, its preparation method and use" (application number 201010022753.4, application date January 13, 2010) patent. technical field [0002] The invention relates to the field of biomedical technology, in particular to the use of staphylococcal protein A (staphylococal protein A, hereinafter referred to as SPA)-antibody trimer as an indicator for testing blood antigens. technical background [0003] SPA can bind to Fc fragments in human and various mammalian serum IgG molecules. The order of binding affinity is pig, dog, rabbit, human, monkey, rat, mouse and cow. In addition to binding to IgG, SPA can also bind to a small amount of IgM and IgA in serum. The adsorption rate of SPA bacteria to various immunoglobulins: 90% to 98% for IgG, 2% to 30% for IgM, and 1.5% for IgA %~20%. [0004] SPA and IgG with co-precipitation react...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/577G01N33/544C12N5/0789
CPCG01N33/6803G01N33/555G01N2333/31
Inventor 张厚亮
Owner 王信
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