115 results about "Antibody formation" patented technology

The present invention relates to a diagnostic device for measuring the ratio of similar structural proteins among the proteins secreted in a liquid test sample taken from diagnosis subject. In further detail, the test device according to the present invention comprises detection marker-antibody conjugate recognizing the same site on two or more similar structural proteins and a detection zone in which antibody specifically recognizes each of said proteins via formation of sandwich type complex, wherein said antibodies form a set, and the present Invention relates to a diagnostic device for early diagnosis of polycystic ovary syndrome, abnormal pregnancy, prostatic carcinoma etc. based on determination of the ratio of follicle stimulating hormone and luteinizing hormone in case of polycystic ovary syndrome, the ratio between hCG isomers in case of abnormal pregnancy, and the ratio of prostate-specific antigens (PSA) in case of prostatic carcinoma.

The present invention relates to a polynucleotide construct encoding a fusion protein consisting of a domain which binds the immunoglobulin Fc region, genetically fused to a truncated form of Pseudomonas exotoxin A (PE). In particular, the invention discloses the fusion protein, ZZ-PE38, and further provides immunotoxins, formed from complexes of the fusion protein with antibodies for targeted cell killing.

The invention provides classes of immunomodulatory compositions which comprise an average of one or more immunostimulatory sequence (ISS) containing polynucleotide conjugated, or attached, to antigen. The extent of conjugation affects immunomodulatory properties, such as extent of antigen-specific antibody form ration, including Th1-associated antibody formation, and thus these various conjugate classes are useful for modulating the type and extent of immune response. The invention also includes methods of modulating an immune response using these compositions.

The invention relates to a protein chip and a kit for detecting abnormal DCP (Des--Carboxy-Prothrombin) in serum and a preparation method thereof, and belongs to the technology of protein detection. The protein chip for detecting the abnormal DCP in the serum is characterized in that substrate slide glass of the protein chip at least comprises a detecting subarea, and one detecting subarea detectsone serum sample; a detecting spot area and a control spot area are formed in the detecting subarea; the detecting spot area has detecting spots formed by fixing a DCP specific antibody; the controlspot area has control spots formed by fixing bovine serum albumin; the concentrations of the substances on all detecting spots in the same detecting spot area are the same; the total amount of the DCPspecific antibody fixed on each of the detecting spot is 3nl; each detecting spot is formed by spraying 10 times, and 300pl is spotted each time. The protein chip and the kit disclosed by the invention can accurately detect the abnormal DCP; in clinical use, the protein chip and the kit have the advantages of high sensitivity, time conservation, convenience and quickness, economy and the like.

The invention belongs to the technical field of biomedical examination, and particularly relates to a calibration product stabilizer, a C peptide determination detection kit and a detection method. The kit comprises a calibration product, a reagent 1, a reagent 2, a reagent R3 and a reagent R4. According to the kit, an acridinium ester-labeled anti-C peptide antibody, an antigen and a horse radishperoxidase-labeled anti-C peptide antibody are utilized to form an antibody-antigen-antibody compound. A triggering agent is added into the compound without a washing process, the compound is continuously detected for a period of time, the peak area is calculated every 0.02-0.05 S, and a dosage-reaction curve is made by using C peptide with known concentration and the calculated peak area; and the content of the C peptide in the sample to be detected is calculated according to the curve. The kit for detecting the C peptide by adopting the spatial proximity chemiluminiscence method provided bythe invention has the advantages of strong calibration product stability, strong reagent anti-interference capability, high accuracy, good specificity and wide linear range, and is suitable for beingused by medical and research institutions at all levels in combination with instrument measurement.

The invention relates to protein peptide powder for enhancing immunity and a preparation method and application thereof. The protein peptide powder is prepared from the following raw materials by weight: 30%-45% of marine fish skin collagen oligopeptide powder, 30%-50% of soybean oligopeptide powder, 10%-20% of Isomaltooligosaccharide, 8%-13% of polydextrose and 0%-0.5% of a sweetener. The protein peptide powder can increase macrophage phagocytic rate and phagocytic index and serum hemolysin content, enhances lymphocyte proliferation and NK cell activity, promotes antibody formation cell number increase, improves the body's immunity, and enhances body immunity.

An immunological analyzing reagent comprising a composition containing a biotin-conjugated antigen or antibody against a substance to be analyzed, and a composition containing an avidin-conjugated microparticle, wherein the biotin-conjugated antigen or antibody contains an amount of biotin which does not substantially agglutinate with the avidin-conjugated microparticle in the absence of the substance to be analyzed, is disclosed. Further, an immunological analyzing method comprising the steps of: bringing into contact a sample, a biotin-conjugated antigen or antibody against a substance to be analyzed, and an avidin-conjugated microparticle, the biotin-conjugated antigen or antibody containing an amount of biotin which does not agglutinate with the avidin-conjugated microparticle in the absence of the substance to be analyzed; and detecting the degree of aggregation between the avidin-conjugated microparticle, and an immunocomplex formed from the substance to be analyzed and the biotin-conjugated antigen or antibody, is disclosed. According to the immunological analyzing reagent and analyzing method, a procedure in which an antibody or antigen is carried on a latex particle is not necessary; self-aggregation of latex particles does not occur; a precise measurement of the substance to be analyzed may be carried out; and an excellent reactivity the same as or superior to that obtained by the conventional sensitized latex method may be obtained.

The invention relates to a mode identification method based on an immune antibody network, which is used for mode identification. The technical scheme of the invention comprises the following steps: generating an original antibody in the immune antibody network by extracting all classes of training samples with a certain number stochastically and completing the initialization of the immune antibody network; taking all the training samples as an input antigen, training the immune antibody network according to an antibody formation algorithm and extracting the mode characteristics of all classesof training samples effectively; and training the immune antibody network repeatedly, stopping training when the continuous training results of two times are accordant, preserving the obtained immuneantibody network and carrying out the mode identification. The invention has simple and convenient calculation and very high correct identification rate without manually setting parameters and thresholds and is suitable for the computer application fields of character identification, fault diagnosis and the like.

The present invention provides a method of quickly and accurately detecting and / or assaying an antigen using fluorescence correlation spectroscopy (FCS), which involves a fluorescence-labeled antibody fragment and a non-fluorescence-labeled intact antibody that form a complex with the antigen. There is a significant difference in diffusion rate between the fluorescence-labeled antibody fragment not bonded to the antigen and the complex formed by the the fluorescence-labeled antibody fragment, the antigen, and the non-fluorescence-labeled intact antibody, and this diffusion rate can be determined using FCS. The antigen can be an antigenic protein, such as an abnormal prion or a harmful protein contained in a food material. According to this method, antigens over a wide scope can be assayed regardless of the shape or molecular weight.

The invention discloses a preparation method of a nanobody. The preparation method comprises the following steps: immunizing animals with a target antigen to obtain peripheral blood mononuclear cellsof the immunized animals, and enriching B cells; marking the target antigen by using FITC fluorescence; culturing the cells by using a semi-solid culture medium to form independent single B cell clones, selecting single B cell clones secreting positive heavy-chain antibodies, extracting total RNAs of positive B cells, carrying out reverse transcription to obtain cDNAs, and carrying out VHH fragment amplification and sequencing; and performing recombinant expression and activity identification of the nanobody. According to the method, the positive B cell clones secreting VHH antibodies can be directly observed and selected, and fluorescence signals are formed through the secreted antibodies, so the secretion functional characteristics of B cell antibodies are better conformed to. The nanobody screened and prepared by the method has the advantages of small molecular weight, strong stability, high affinity, good solubility, easiness in expression and the like, and has a wide application scope.