Application of Glypican-1 protein in diagnosis of pancreatic cancer, detection method of positive exosome concentration, and use of detection method
A detection method, exosome technology, applied in the field of biomedicine, can solve the problem of evaluating the value of pancreatic cancer without Glypican-1 protein
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Embodiment 1
[0044] Glypican-1 protein is used to prepare products for the diagnosis of pancreatic cancer. Wherein, the product is a chip, a detection reagent or a detection kit.
[0045]Preferably, the chip includes a protein chip, and the detection kit includes a protein immunodetection kit.
Embodiment 2
[0047] A method for detecting the concentration of Glypican-1 positive exosomes is a nanoplasmon enhanced scattering method.
[0048] The detection reagent used in the nano-plasmon-enhanced scattering method includes the following components:
[0049] (I) Amino-functionalized glass slides (NH 2 glass), methanol, nitrogen (N2) flow, 50-well polydimethylsiloxane membrane (PDMS);
[0050] (II) Phosphate Buffered Washing Solution (PBS), pH 7.4, its components are: 140 mM NaCl, 2.7 mM KCl, 10 mM Na 2 HPO 4 , 1.8mM KH 2 PO 4 ;
[0051] (III) The blocking solution is PBS containing 5% bovine serum albumin (BSA), the pH value is 7.4, and its components are: PBS, 5% BSA;
[0052] (IV) Carboxyl functionalized gold nanoparticles (GNP): 50nm gold nanospheres (radiation green light), 25×60nm gold nanorods (radiation red light), when the two are combined, the signal is enhanced and the yellow light is emitted;
[0053] (V) MES solution, the pH value is 4.7, and its composition is: 2-...
Embodiment 3
[0076] The above-mentioned nanoplasma-enhanced scattering method for detecting the concentration of Glypican-1 positive exosomes was used to detect the content of Glypican-1 protein positive exosomes in the serum of pancreatic cancer for the diagnosis and staging of pancreatic cancer.
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