Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Kit and system for predicting susceptibility of malignant tumor of ovary

A technology of ovarian malignancy and susceptibility, applied in the field of ovarian malignancy susceptibility prediction kit and ovarian malignancy susceptibility prediction system, STR kit, can solve unscientific, impossible early warning of tumor susceptibility, impossible And other issues

Active Publication Date: 2018-11-23
JILIN UNIV
View PDF12 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the occurrence and development of tumors is a very complicated process, and it is obviously impossible and unscientific to use the changes of a single molecular genetic marker to diagnose the disease
With the existing technical means, it is not possible to provide a more accurate early warning of tumor susceptibility only through genetic information, and the current early identification and prediction methods for tumors need to be improved

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Kit and system for predicting susceptibility of malignant tumor of ovary
  • Kit and system for predicting susceptibility of malignant tumor of ovary
  • Kit and system for predicting susceptibility of malignant tumor of ovary

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0108] Example 1 A Prediction System for Ovarian Malignant Tumor Susceptibility

[0109] A susceptibility prediction system for ovarian malignancies, comprising:

[0110] A device for obtaining the number of repetitions of the STR site short tandem sequence of the sample DNA;

[0111] Data processing and judging device, it comprises following module (as figure 1 ):

[0112] The data input module is used to input the age, gender, and repeat times of the short tandem sequence of the subject;

[0113] The database management module is used for the operation management of data storage, modification, deletion, query and printing;

[0114] The data calculation module is used to calculate the result of the discriminant function according to the number of repetitions of the STR site short tandem sequence in the data input module;

[0115] The analysis, discrimination and result output module is used to compare the results of the discriminant function, so as to predict the suscept...

Embodiment 2

[0149] Embodiment 2 A kind of ovarian malignant tumor susceptibility prediction kit

[0150] A kit for predicting susceptibility to malignant ovarian tumors, comprising the following components: STR-1 primers, STR-2 primers, STR-3 primers, STR-4 primers, STR-5 primers, STR-6 primers, PCR amplification Reaction solution, LIZ-500 molecular weight internal standard, deionized formamide, instruction manual.

[0151] The concentrations of the above STR-1 primers, STR-2 primers, STR-3 primers, STR-4 primers, STR-5 primers, and STR-6 primers are all 10 μM, and the primer sequences are shown in the following table:

[0152]

[0153]

[0154] The PCR amplification reaction solution is a mixture of the following reagents: TaqDNA polymerase (5U / μL), Tris-HCl (100mM, pH 8.8 at 25°C), KCl (500mM), ethylphenyl polyethylene glycol (0.8 %(v / v)), MgCl 2 (25mM), dNTP (10mM), deionized water.

[0155] The PCR amplification reaction solution is stored at -20°C; the LIZ-500 molecular weig...

Embodiment 3

[0157] Example 3 Using the system of Example 1 and the kit of Example 2 to predict the risk of the subject suffering from ovarian cancer

[0158] Subject: Female, 58 years old, was treated in the Department of Obstetrics and Gynecology of the Second Hospital of Jilin University. After being fully informed of the purpose and use of the examination and voluntary, she signed an informed consent and collected 1 mL of anticoagulant blood through a peripheral vein.

[0159] Use the kit of embodiment 2, carry out the following steps according to the method recorded on the kit instruction manual:

[0160] (1) Extract sample DNA: Use a DNA extraction kit to extract blood genomic DNA;

[0161] (2) PCR reaction

[0162] (2-1) Take out STR-1 primer, STR-2 primer, STR-3 primer, STR-4 primer, STR-5 primer, STR-6 primer, PCR amplification reaction solution from the refrigerator, equilibrate to room temperature, each After the components are fully dissolved, quickly centrifuge for 10 second...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a kit for predicting the susceptibility of a malignant tumor of an ovary. The kit is prepared from the following components: an STR (Short Tandem Reapeats)-1 primer, an STR-2primer, STR-3 primer, STR-4 primer, STR-5 primer and STR-6 primer; further, the kit is also prepared from a PCR (Polymerase Chain Reaction) amplification reaction solution, an LIZ-500 molecular weightinterior label and deionized formamide. The kit for predicting the susceptibility of the malignant tumor of the ovary, disclosed by the invention, can be used for diagnosing the malignant tumor of the ovary and predicting the susceptibility. The invention also provides a system for predicting the susceptibility of the malignant tumor of the ovary.

Description

technical field [0001] The present invention relates to the field of biomedicine. Specifically, it relates to an ovarian malignancy susceptibility prediction kit and an ovarian malignancy susceptibility prediction system. More specifically, the present invention relates to a kit for detecting STRs of ovarian cancer susceptibility-related genes through short tandem repeats (Shorttandem repeats, referred to as STR) site fragment analysis methods, and by combining discriminant analysis statistical methods, thereby Early warning of the subject's susceptibility to ovarian malignancies. Background technique [0002] Tumor is a disease closely related to genetics. To study the molecular genetic basis of tumors, and then propose tumor-specific genetic markers, it is hoped that it can be used for routine detection, clinical diagnosis, personalized treatment, and disease tracking after recovery. Etc. provides a simple and feasible method. However, the variability of individual pati...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/6886C12Q1/6869G06F19/18G06F19/24
CPCC12Q1/6869C12Q1/6886C12Q2600/156C12Q2525/151C12Q2531/113C12Q2537/165
Inventor 任明郝书弘王晓峰杨麒巍
Owner JILIN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com