Liver cirrhosis detection kit and detection method thereof
A technology for detection kits and detection reagents, which is applied in the biological field and can solve problems such as reliability discounts
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[0032] The following describes specific embodiments created by the present invention:
[0033] A detection reagent for liver cirrhosis, including the following reagents: CHI3L1 microtiter plate, CHI3L1 detection antibody, HRP-labeled avidin, HRP-labeled avidin diluent, CHI3L1 sample diluent, developer A, Toner B, washing solution, stop solution, calibrator and quality control products.
[0034] The developer A is a buffer solution containing hydrogen peroxide, and the developer B is a buffer solution containing 3,3',5,5'-tetramethylbenzidine. The microwells of the CHI3L1 microtiter plate are coated with rat anti-CHI3L1 monoclonal antibodies, and the CHI3L1 microtiter plate is provided with 8 microwells. The CHI3L1 detection antibody is a biotin-labeled goat anti-CHI3L1 polyclonal antibody. The stop solution is 1 mol / L sulfuric acid solution.
[0035] The concentration of CHI3L1 in the calibrator is as follows: the fixed value of the concentration is derived from LC-MS measurement....
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