SPA-antibody tripolymer, cell treating kit containing tripolymer, preparation method and application thereof

Abstract

The invention relates to an SPA-antibody tripolymer, a cell treating kit containing the tripolymer, a preparation method and application thereof. The tripolymer comprises an anti erythrocyte antibody, an SPA and an antibody of a certain anti leukocyte antigen or other antigens. The tripolymer is combined with an erythrocyte through the anti erythrocyte antibody per se, the other antibody is combined with a corresponding leukocyte antigen (or other antigens), and then leukocyte (other cells, factors) and the erythrocyte are deposited through an erythrocyte sedimentation process or other methods of density gradient centrifugation and the like, thereby achieving the purpose of eliminating ingredients of corresponding cells and the like, connecting the erythrocyte with a corresponding antigen by the SPA, and being used as a mean for detecting a certain antigen. The invention has convenience and simpleness, and can be directly used for clinically separating and extracting stem cells/progenitor cells; and the collected and extracted stem cells/progenitor cells have no external markers. Meanwhile, the kit based on the tripolymer can be industrially produced so as to realize the popularization of the separation and purification technology of hematopoietic stem cells.

Description

SPA-antibody trimer, cell treatment kit containing the trimer, preparation method and use thereof technical field The invention relates to the field of biomedical technology, in particular to a staphylococcal protein A (staphylococal protein A, hereinafter referred to as SPA)-antibody trimer, a cell treatment kit based on the polymer, and a preparation method and use thereof. technical background SPA can bind to Fc fragments in human and various mammalian serum IgG molecules. The order of binding affinity is pig, dog, rabbit, human, monkey, rat, mouse and cow. In addition to binding to IgG, SPA can also bind to a small amount of IgM and IgA in serum. The adsorption rate of SPA bacteria to various immunoglobulins: 90% to 98% for IgG, 2% to 30% for IgM, and 1.5% for IgA %~20%. SPA and IgG with co-precipitation reaction must have two binding sites, which are respectively on the Fc and Fab segments. Although the lack of one can bind to SPA, co-precipitation reaction does no...

AI Technical Summary

Problems solved by technology

The common problems of these methods are: firstly, all types of stem cells cannot be effectively preserved; secondly, the