7 results about "Immune recognition" patented technology

Provided herein are CD70-targeted chimeric antigen receptors (CARs), genetically engineered cells such as T cells containing the same, and related methods and uses of the genetically engineered cells in allogeneic cell therapy. Also provided are T cells that are genetically engineered with a CAR, such as a CD70-targeted CAR, and are further genetically engineered by one or more strategies to reduce host immune recognition of the engineered T cells, such as by heterologous expression of one or more additional transgenes and by genetic disruption to reduce or eliminate expression or one or more endogenous protein. Also provided are methods of making and using the engineered T cells for cell therapy, including in connection with cancer immunotherapy comprising adoptive transfer of the engineered T cells.

The present application relates to a kind of encoding aspergillus fumigatus chimeric antigen circular RNA and its application, belong to biomedical and vaccine technology field.The present application first applies circular RNA technology to anti-aspergillus fumigatus infection, provides new strategy for fungal vaccine development.Select the non-transmembrane region of aspergillus fumigatus cell membrane protein FtrA to construct antigen, avoid the transmembrane region expression problem and immune recognition uncertainty;Fusion IgK signal peptide and Fc domain, significantly improve antigen secretion efficiency, stability and immune presentation effect.LNP delivery system mature, efficient, can effectively deliver vaccine to host cell and express antigen, the LNP@CircRNA provided by the present application, especially the LNP@CircRNA FtrA2 , can induce high level of IgG antibody and T cell immune response in mouse in vivo without using adjuvant, realize the dual activation of humoral immunity and cellular immunity.

The application provides a gold-silver alloy nanoflower SERS substrate and a preparation method and application thereof. 3+ and Ag + The preparation method is as follows: an aluminum foil paper substrate is placed in a growth solution containing Au 3+ and Ag + , a gold-silver alloy nanoflower array pattern layer with a multi-level branch structure is formed on the surface of the aluminum foil paper substrate by a displacement reaction of aluminum and noble metal ions in situ. The gold-silver alloy nanoflower array pattern layer has rich nanotips and a three-dimensional rough structure, can form a large number of electromagnetic 'hot spots', and can significantly enhance the Raman signal intensity. Relying on the excellent SERS enhancement performance, the gold-silver alloy nanoflower SERS substrate is applied to an immune recognition mediated sandwich detection mode, and high-sensitivity and accurate detection of a hidden blood marker in a fecal sample is realized. The preparation process is simple, the cost is low, and complex equipment is not needed; the obtained SERS substrate has strong stability and excellent repeatability, and can meet the needs of early screening and on-site rapid detection of digestive diseases.

This invention relates to a method for predicting T lymphocyte antigen binding based on residual graph attention, belonging to the field of bioinformatics. First, a heterogeneous graph structure and node features of T lymphocytes and antigens are constructed through preprocessing. A two-layer graph attention network with residual connections is used to encode cross-molecular features. Then, a difficult negative sampling strategy is introduced to screen high-confidence negative samples, improving the ability to distinguish boundary samples. Simultaneously, a pairwise AUC loss function is used to optimize ranking performance, and three optimizers—Adam, PESG, and SGD—are combined for collaborative updates to achieve simultaneous improvement in local convergence and global ranking ability. Furthermore, the weights of positive samples are fixed outside the training loop. This invention significantly improves the prediction performance of T lymphocyte-antigen binding, effectively solving problems such as extreme sample imbalance, performance instability, and redundant training computation, providing a reliable and efficient computational tool for research on immune recognition mechanisms and precision immunotherapy.

This invention provides a gold-silver alloy nanoflower SERS substrate, its preparation method, and its application. The preparation method involves placing an aluminum foil paper substrate in an Au-containing... 3+ and Ag + In a growth solution, a gold-silver alloy nanoflower array pattern layer with a multi-level branched structure is grown in situ on the surface of an aluminum foil substrate using a displacement reaction between aluminum and noble metal ions. This gold-silver alloy nanoflower array pattern layer has abundant nano-tip and three-dimensional rough structure, which can form a large number of electromagnetic "hot spots" and significantly enhance the Raman signal intensity. Based on its excellent SERS enhancement performance, this gold-silver alloy nanoflower SERS substrate is applied to the immune recognition-mediated sandwich detection mode to achieve highly sensitive and accurate detection of occult blood markers in fecal samples. The preparation process of this invention is simple, low-cost, and requires no complex equipment. The resulting SERS substrate has strong stability and excellent repeatability, which can meet the needs of early screening and rapid on-site detection of digestive tract diseases.

Provided herein are CD70-targeted chimeric antigen receptors (CARs), genetically engineered cells such as T cells containing the same, and related methods and uses of the genetically engineered cells in allogeneic cell therapy. Also provided are T cells that are genetically engineered with a CAR, such as a CD70-targeted CAR, and are further genetically engineered by one or more strategies to reduce host immune recognition of the engineered T cells, such as by heterologous expression of one or more additional transgenes and by genetic disruption to reduce or eliminate expression or one or more endogenous protein. Also provided are methods of making and using the engineered T cells for cell therapy, including in connection with cancer immunotherapy comprising adoptive transfer of the engineered T cells.