15 results about "Immune recognition" patented technology

The application discloses a kind of rice mycotic acid hapten and antigen preparation application, it is related to antigen preparation technical field, introduce sulfydryl in the 2 of rice mycotic acid, and obtain rice mycotic acid antigen by coupling with protein through connecting arm, rice mycotic acid hapten and antigen preparation application include the following specific flow: S1, the synthesis of rice mycotic acid hapten;S2, the preparation of rice mycotic acid immunogen;S3, the preparation of rice mycotic acid coating original;S4, the preparation of rice mycotic acid monoclonal antibody;S5, the preparation of rice mycotic acid immunofluorescence detection card;S6, rice mycotic acid detection in food;S7, detection limit experiment;S8, false positive rate, false negative rate experiment.The rice mycotic acid hapten retains the structural characteristics of rice mycotic acid, better exposes rice mycotic acid structural characteristics, improves the immunity and immune recognition ability of antigen, lays foundation for preparing specific, high sensitivity antibody, and improves its rapid detection ability.

Provided herein are CD70-targeted chimeric antigen receptors (CARs), genetically engineered cells such as T cells containing the same, and related methods and uses of the genetically engineered cells in allogeneic cell therapy. Also provided are T cells that are genetically engineered with a CAR, such as a CD70-targeted CAR, and are further genetically engineered by one or more strategies to reduce host immune recognition of the engineered T cells, such as by heterologous expression of one or more additional transgenes and by genetic disruption to reduce or eliminate expression or one or more endogenous protein. Also provided are methods of making and using the engineered T cells for cell therapy, including in connection with cancer immunotherapy comprising adoptive transfer of the engineered T cells.

The invention discloses a flow type single molecule detection probe and method based on rolling circle amplification and application in the technical field of biomolecule detection. The flow type single molecule detection probe comprises a plurality of groups of magnetic bead detection probes and antibody binding probes which are used in pairs, each magnetic bead detection probe comprises a superparamagnetic microsphere, and the surface of the superparamagnetic microsphere is covalently connected with a plurality of fixed antibody probes; each fixed antibody probe comprises a first antibody and a first fixed DNA chain; each antibody binding probe comprises a second antibody and a second fixed DNA chain; aiming at the same target antigen, the first antibody and the second antibody in the same group can be specifically combined with different epitopes of the target antigen to form a sandwich immune complex. According to the invention, the hairpin structure DNA probe is combined with the adjacent ligation and rolling circle amplification technology to form dual specific screening of immune recognition and DNA ligation, so that the signal-to-noise ratio and sensitivity of detection are remarkably improved, and the design complexity, development cost and cross reaction risk of a multi-target detection system are greatly reduced.

According to the echinococcus granulosus vaccine based on the self-assembly peptide, a peptide fragment, rich in dominant epitopes, in EgG1Y162-2 is connected to a Q11 amino terminal through '-SGSG-', due to the self-assembly effect of the Q11 peptide, the peptide fragment rich in dominant epitopes is repeatedly expressed on the surface of nanofibers, and therefore EgG1Y162 dominant multi-epitope self-assembly nano-polypeptide is formed; the EgG1Y162 dominant multi-epitope self-assembled nano polypeptide is adopted in the vaccine, so that the immune recognition and response of a body are effectively enhanced.

The invention discloses an intelligent artificial bionic DC cell nanoparticle nDC-M and a preparation method, application and a detection method thereof. The nano-particle is of a core-shell structure, the inner core is a hypoxia response type nano-micelle of a sound-sensitive agent SP8 and IL-2 for activating T cells to be mature, the outer layer is coated with a mature DC cell membrane, and the nano-particle has the immune recognition characteristic and the treatment function. In a tumor hypoxic microenvironment, the micelle specifically releases SP8 and IL-2; sP8 generates ROS through ultrasonic excitation, tumor cell immunogenicity death is induced, and antigens are released; iL-2 activates proliferation and functions of tumor infiltration lymphocytes; the outer DC cell membrane and the antigen capture functional group enhance the collection and activation of immune cells through surface antigen presentation, and amplify the anti-tumor immune response. By combining sonodynamic therapy and immunoregulation, tumor growth is inhibited, metastatic load is reduced, good biocompatibility and safety are achieved, and a novel bionic delivery system is provided for metastatic tumor immune combined therapy.

The present application relates to a kind of encoding aspergillus fumigatus chimeric antigen circular RNA and its application, belong to biomedical and vaccine technology field.The present application first applies circular RNA technology to anti-aspergillus fumigatus infection, provides new strategy for fungal vaccine development.Select the non-transmembrane region of aspergillus fumigatus cell membrane protein FtrA to construct antigen, avoid the transmembrane region expression problem and immune recognition uncertainty;Fusion IgK signal peptide and Fc domain, significantly improve antigen secretion efficiency, stability and immune presentation effect.LNP delivery system mature, efficient, can effectively deliver vaccine to host cell and express antigen, the LNP@CircRNA provided by the present application, especially the LNP@CircRNA FtrA2 , can induce high level of IgG antibody and T cell immune response in mouse in vivo without using adjuvant, realize the dual activation of humoral immunity and cellular immunity.

The invention provides a design method for improving the population coverage rate of a multi-epitope vaccine, which comprises the following steps: carrying out epitope prediction on B cells and T cells according to candidate proteins to obtain B cell epitopes and T cell epitopes; setting a sliding window for the candidate antigen protein; judging whether the needed epitope exists in the sliding window or not; if the needed epitope exists, shortening the length of the window by one amino acid, and judging whether the specifically bound MHC-I / II allele is reduced or not; if the MHC-1 / II allele is reduced, the window shortening operation is revoked, and overlapping epitopes which are specifically combined with the maximum MHC-1 / II allele are reserved, so that candidate overlapping epitopes are obtained. According to the invention, a three-type epitope maximization strategy based on a sliding window is adopted, and population coverage rate maximization and dual immune recognition are realized in a vaccine construct with a compact structure by recognizing the number of specific binding MHC-I / II alleles and the number of partially overlapped B cell epitope amino acids.

The application provides a gold-silver alloy nanoflower SERS substrate and a preparation method and application thereof. 3+ and Ag + The preparation method is as follows: an aluminum foil paper substrate is placed in a growth solution containing Au 3+ and Ag + , a gold-silver alloy nanoflower array pattern layer with a multi-level branch structure is formed on the surface of the aluminum foil paper substrate by a displacement reaction of aluminum and noble metal ions in situ. The gold-silver alloy nanoflower array pattern layer has rich nanotips and a three-dimensional rough structure, can form a large number of electromagnetic 'hot spots', and can significantly enhance the Raman signal intensity. Relying on the excellent SERS enhancement performance, the gold-silver alloy nanoflower SERS substrate is applied to an immune recognition mediated sandwich detection mode, and high-sensitivity and accurate detection of a hidden blood marker in a fecal sample is realized. The preparation process is simple, the cost is low, and complex equipment is not needed; the obtained SERS substrate has strong stability and excellent repeatability, and can meet the needs of early screening and on-site rapid detection of digestive diseases.

The invention belongs to the technical field of microorganisms and genetic engineering. The invention provides a listeria monocytogenes strain as well as a preparation method and application thereof, the flagellum formation ability of the strain is enhanced, the environmental stress resistance is enhanced, and the immune recognition of host cells on listeria monocytogenes can be enhanced; the strain is subjected to delta yxeA gene deletion mutation; the bacterial strain is named as Listeria monocytogenes Lm-delta lmo2568, the preservation number of the bacterial strain is CGMCC (China General Microbiological Culture Collection Center) No.36001, and the bacterial strain is preserved in the China General Microbiological Culture Collection Center on September 22, 2025. The invention finds that the deletion of Listeria monocytogenes YxeA promotes the formation and expression of flagellum, and the obtained strain can enhance the immune recognition of host cells on Listeria monocytogenes, and can be used for preparing related preparations.

This invention relates to a method for predicting T lymphocyte antigen binding based on residual graph attention, belonging to the field of bioinformatics. First, a heterogeneous graph structure and node features of T lymphocytes and antigens are constructed through preprocessing. A two-layer graph attention network with residual connections is used to encode cross-molecular features. Then, a difficult negative sampling strategy is introduced to screen high-confidence negative samples, improving the ability to distinguish boundary samples. Simultaneously, a pairwise AUC loss function is used to optimize ranking performance, and three optimizers—Adam, PESG, and SGD—are combined for collaborative updates to achieve simultaneous improvement in local convergence and global ranking ability. Furthermore, the weights of positive samples are fixed outside the training loop. This invention significantly improves the prediction performance of T lymphocyte-antigen binding, effectively solving problems such as extreme sample imbalance, performance instability, and redundant training computation, providing a reliable and efficient computational tool for research on immune recognition mechanisms and precision immunotherapy.

The present disclosure provides immunogenic compositions and methods for vaccination with a CelTOS immunogen. The immunogenic composition comprises Babesia, Theileria or Cytauxzoon CelTOS. The immunogenic composition may also comprise CelTOS with structural changes that affect immune recognition.

This invention provides a gold-silver alloy nanoflower SERS substrate, its preparation method, and its application. The preparation method involves placing an aluminum foil paper substrate in an Au-containing... 3+ and Ag + In a growth solution, a gold-silver alloy nanoflower array pattern layer with a multi-level branched structure is grown in situ on the surface of an aluminum foil substrate using a displacement reaction between aluminum and noble metal ions. This gold-silver alloy nanoflower array pattern layer has abundant nano-tip and three-dimensional rough structure, which can form a large number of electromagnetic "hot spots" and significantly enhance the Raman signal intensity. Based on its excellent SERS enhancement performance, this gold-silver alloy nanoflower SERS substrate is applied to the immune recognition-mediated sandwich detection mode to achieve highly sensitive and accurate detection of occult blood markers in fecal samples. The preparation process of this invention is simple, low-cost, and requires no complex equipment. The resulting SERS substrate has strong stability and excellent repeatability, which can meet the needs of early screening and rapid on-site detection of digestive tract diseases.

The invention provides a bimodal cortisol immunodetection kit and a detection method thereof. The kit comprises a coated 96-well plate, a multifunctional nano immune receptor solution, a cortisol antibody solution, a washing buffer solution and a TMB (Tetramethylbenzidine) developing solution. Wherein a specific antibody is loaded on the surface of the multifunctional nano immune receptor, and the multifunctional nano immune receptor has peroxidase-like catalytic activity and endogenous fluorescence emission characteristics at the same time. In the detection process, a detected sample and a cortisol antibody solution are mixed with the multifunctional nano immune receptor after being incubated, a TMB developing solution is added after incubation is continued, a colorimetric signal and a fluorescence signal are synchronously obtained through the same reaction system, and the sum of the ratio of the colorimetric signal and the fluorescence signal is used as a quantitative criterion of the cortisol concentration. Immune recognition, signal catalysis and bimodal output are integrated in a single nano immune receptor, matrix interference is effectively reduced, detection precision and stability are improved, and the method is suitable for high-reliability quantitative detection of cortisol in complex body fluid.

Provided herein are CD70-targeted chimeric antigen receptors (CARs), genetically engineered cells such as T cells containing the same, and related methods and uses of the genetically engineered cells in allogeneic cell therapy. Also provided are T cells that are genetically engineered with a CAR, such as a CD70-targeted CAR, and are further genetically engineered by one or more strategies to reduce host immune recognition of the engineered T cells, such as by heterologous expression of one or more additional transgenes and by genetic disruption to reduce or eliminate expression or one or more endogenous protein. Also provided are methods of making and using the engineered T cells for cell therapy, including in connection with cancer immunotherapy comprising adoptive transfer of the engineered T cells.

According to the preparation method, the SERS substrate material with a highly ordered arrangement structure is prepared by taking gold nanorods as a core construction unit and adopting a phase transfer induced self-assembly strategy; a high-sensitivity SERS (Surface Enhanced Raman Scattering) analysis chip aiming at a nervous system injury marker GFAP (Glial Fibroblast Acid Protein) is constructed by further capturing an antibody through immobilized GFAP (Glial Fibroblast Acid Protein), an SERS probe with good immune recognition specificity is designed and prepared, and the SERS probe can be specifically combined with a target antigen. Based on the SERS platform, rapid, accurate and quantitative detection of the GFAP biomarker in the whole blood biological sample is realized, and the average detection time is only 5 minutes.