Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for constructing spiral immunization assembly sensor for determining neomycin

A construction method, neomycin technology, applied in the field of material chemistry

Active Publication Date: 2012-05-02
MEIZHENG BIO TECH CO LTD
View PDF2 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it is still blank to use this assembly system combined with the principle of immune recognition to construct small molecule detection sensors.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for constructing spiral immunization assembly sensor for determining neomycin
  • Method for constructing spiral immunization assembly sensor for determining neomycin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] (1) Preparation of water-soluble helical polymer

[0032] A, the preparation of methyl 4-aminobenzoate

[0033] 2.6g of methyl 4-bromobenzoate, 113mg of tributylphosphine, 321g of bis(dibenzylideneacetone)palladium and 3.75g of bis-(trimethylsilyl)amide lithium were placed in a 50mL Erlenmeyer flask, argon Gas protection, add 26mL of anhydrous toluene. The Erlenmeyer flask was sealed with a rubber stopper, the mixture was stirred at room temperature, and the reactant was monitored by TLC. After two days of reaction, the crude product was diluted by adding 100 mL of diethyl ether, at the same time, a small amount of hydrochloric acid was added, and then 300 mL of diethyl ether was added and stirred. The precipitate of methyl 4-aminobenzoate was washed with ether and dried in air, then 100 mL of 2M NaOH solution was added, and the mixture was washed with 100 mL of CH 2 Cl 2 extraction. Then add 100 mL of saturated NH 4 Cl solution was diluted. The organic layer was...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for constructing a spiral immunization assembly sensor for determining neomycin, belonging to the technical field of material chemistry. The method comprises the following steps: preparing a water-soluble spiral polymer, modifying the spiral polymer with semi-antigen, coupling gold nano particles with antibody, and constructing a neomycin sensor. The invention provides a water-soluble spiral polymer, that is, poly(4-isocyano-benzoic acid). According to the invention, by immunization identification (neomycin semiantigen modified spiral polymer) and gold nanoparticle (coupled with antibody) assembly, the circular dichroism spectrum (CD) signals generated by the assembly detect the neomycin.

Description

technical field [0001] The invention discloses a helical immune assembly sensor for detecting neomycin, which belongs to the technical field of material chemistry. Background technique [0002] Helices exist widely in nature, and natural macromolecules such as DNA, protein, collagen, etc. will present magical helical structures, and scientists have found that helical structures are an important factor for these molecules to exhibit biological activity. Fascinated by these magical helical structures, scientists designed and synthesized some helical polymers. Macromolecules with helical structures are uniquely attractive to polymer scientists because of the unique properties and wide-ranging applications of synthesized helical polymers. Potential uses of helical macromolecules include: molecular recognition, chiral separation, chiral catalysis, data storage, display materials, etc. [0003] Nanoparticles (Nanoparticles, Nas) generally refer to particles with at least one dim...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N33/53
Inventor 王利兵胥传来许宙
Owner MEIZHENG BIO TECH CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com