Grass carp bacteria small peptide recognition receptor as well as preparation method and application thereof

Abstract

The invention discloses a grass carp bacteria small peptide recognition receptor and a preparation method and application thereof, the grass carp bacteria small peptide recognition receptor is grass carp NOD2-LRR protein, the amino acid sequence is as shown in SEQ ID NO.1, and the cDNA sequence of the coding gene is as shown in SEQ ID NO.2. The preparation method of the grass carp bacterium small peptide recognition receptor comprises the following steps: cloning a grass carp NOD2 gene, expressing, purifying and renaturating. In the invention, an immune recognition model of grass carp NOD2 on bacterial MDP is established by using a molecular docking method for the first time, NOD2-LRR protein is successfully obtained by using a prokaryotic expression system, and the result shows that the NOD2-LRR protein has binding activity on bacterial MDP, can specifically recognize bacterial MDP, and plays an important role in immune recognition of bacterial MDP; the method has potential application value in the aspects of developing novel antibacterial agents, immunopotentiators, feed additives and the like.

Description

technical field [0001] The invention relates to a grass carp bacterial small peptide recognition receptor and a preparation method and application. Background technique [0002] Bacterial enteritis in fish is known for its wide incidence (involving almost all farmed fish), serious harm (high infection rate and mortality rate), and complex pathogenesis (affected by factors such as host, pathogen and environment). It has attracted much attention and has become a hot and difficult point in the field of aquatic disease and immunology research. For the pathogenic bacteria of fish intestinal infection, the early immunological theory believed that the body can use its own innate immune system (Innate immunity system) to recognize foreign pathogens and the danger warning signals generated by them, induce the body to produce an immune response, and finally eliminate it. Exogenous pathogenic substances to maintain intestinal homeostasis. Studies have shown that innate immunity is th...

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Problems solved by technology

[0004] At present, studies on the expression and activity of NOD2 in lower vertebrate fish are relatively scarce

At the same time, compared with higher animals, because fish NOD2 has a large difference in structure, it is not clear whether the pathogenic pattern recognition receptor protein NOD2 is involved in the bacterial enteritis response of fish.

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