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Photoelectric chemical detection method of zearalenone based on TiO2 mesocrystal

A technology of zearalenone and mesoscopic crystals, applied in the direction of electrochemical variables of materials, can solve the problems of low photoelectric conversion efficiency and large forbidden band width, and achieve the effect of improving specificity and sensitivity

Inactive Publication Date: 2017-02-08
FUJIAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, TiO 2 The band gap is large and can only be excited by ultraviolet light, so the photoelectric conversion efficiency is low in the visible light region

Method used

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  • Photoelectric chemical detection method of zearalenone based on TiO2 mesocrystal
  • Photoelectric chemical detection method of zearalenone based on TiO2 mesocrystal
  • Photoelectric chemical detection method of zearalenone based on TiO2 mesocrystal

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Experimental program
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Effect test

Embodiment 1

[0030] A TiO-based 2 The preparation method of the photoelectrochemical sensor of mesotube crystal (such as figure 1 shown):

[0031] (1) Pretreatment of the glassy carbon electrode: the glassy carbon electrode is first mechanically polished and polished on the suede covered with alumina powder, washed with secondary water to remove the residual powder on the surface, and then moved into an ultrasonic water bath for cleaning until it is cleaned, and finally Wash thoroughly with ethanol, dilute acid and water;

[0032] (2) Add dropwise 4 μL of RTM suspension with a concentration of 3 mg / ml on the surface of a clean glassy carbon electrode, dry it under infrared light, and cool to room temperature;

[0033] (3) Immerse the electrode in 0.5 mg / ml PDA solution for 30 min, and dry it at room temperature;

[0034] (4) Put the modified electrode in 5 μLAb 1 (2 mg / m) solution and incubated at 37°C for 1h, then washed with pH 7.5 phosphate buffer solution to remove excess Ab 1 The...

Embodiment 2

[0037] Rutile TiO 2 Preparation of mesoscopic crystal (RTM) materials:

[0038] 0.5 g sodium dodecylbenzenesulfonate (SDBS) dissolved in 25 mL 2.2 mol / ml HNO 3 solution, stirred for 15 minutes. Then 0.5 mL titanium(IV) isopropoxide was added and stirred at 80 °C for 48 h. Subsequently, the obtained product was centrifuged, washed 4-5 times with ultrapure water and ethanol, and dried overnight at 60 °C. The above product was calcined in the air at 400 °C for 1 h to remove residual organic matter to obtain rutile TiO 2 mesoscopic crystals. Electron emission scanning electron microscope (SEM) image of RTM, as shown in Figure 2A, RTAM is a porous structure with a size of 90-130 nm. Transmission electron microscope (TEM) image and selected area electron diffraction (SAED) image of RTM, as shown in Figure 2 B and Figure 2B As shown in the inset, it is shown that the rutile TiO 2 The formation of mesogens.

Embodiment 3

[0040] Preparation of Mesoporous Cobalt Tetroxide (OMCO) Solution:

[0041] Preparation of mesoporous cobalt tetroxide (OMCO): 3 g of KIT-6 molecular sieve was added to 30 mL of 0.84 mol / L Co(NO 3 ) 2 •6H 2 O in ethanol and evaporated to dryness at 80 °C. Repeat the above steps. Finally, the material was calcined at 450 °C for 6 h. KIT-6 hard template was removed with 2 mol / L NaOH solution, sodium silicate was removed by centrifugation, and the sample was dried at 100°C to obtain 3d-Co 3 o 4 powder. The electron emission scanning electron microscope (SEM) image of OMCO, shown in Fig. 2C, shows that OMCO has a periodic mesoporous network structure. Transmission electron microscopy (TEM) images of OMCO, such as Figure 2D As shown, it shows that OMCO has a regular and ordered mesoporous structure, which can immobilize more biomacromolecules, so that it can effectively improve the sensitivity of PEC biosensors.

[0042] Mesoporous cobalt tetroxide labeled secondary antib...

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Abstract

The invention discloses a photoelectric chemical detection method of zearalenone based on a TiO2 mesocrystal. According to the method, a polydopamine-sensitized rutile type TiO2 mesocrystal is used as a photoelectric active substrate material and is applied to an immobilization zearalenone antibody; a mesoporous cobaltosic oxide marked zearalenone secondary antibody is used as a signal probe; a photoelectric chemical sensor based on a sandwich immune recognition mode is prepared by using a signal amplification effect of the probe on the substrate material and is applied to quantitative detection of zearalenone; based on stability and high conductivity of polydopamine, polydopamine and an RTM composite can accelerate transferring speed of photoinduced electrons and increasing photocurrent signals; through a sandwich immune recognition process, OMCO with competitive light-harvesting capability is introduced into a sensing interface; and high-sensitivity detection on zearalenone in a concentration range of 1x10<-6>ng / Ml-20ng / mL can be achieved.

Description

technical field [0001] The invention belongs to the technical field of novel functional materials and biosensing detection, and specifically relates to a TiO-based 2 Photoelectrochemical detection of zearalenone in mesoscopic crystals. Background technique [0002] Zearalenone (Zearalenone), also known as F-2 toxin, is a metabolite of Gibberella zeae. It is widely distributed in contaminated grains, agricultural by-products, and dairy products, especially corn and its processed products. Zearalenone has reproductive and developmental toxicity, immunotoxicity and strong teratogenic toxicity, etc. It can also affect endocrine and may induce tumors. At present, the bioassay methods for the detection of zearalenone mainly include enzyme-linked immunosorbent assay, electrochemistry, and fluorescence spectroscopy. However, the further enhancement of the sensitivity of these methods and the exploration of a sensing platform with low background signal, good reproducibility, and w...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/30G01N27/38
CPCG01N27/30G01N27/38
Inventor 戴宏汪慧霞高利红林燕语
Owner FUJIAN NORMAL UNIV
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