10 results about "Human lymphocyte" patented technology

The invention belongs to the technical field of cell culture, and particularly relates to a method for preparing Jurkat cells on a large scale by utilizing a bioreactor. The preparation method comprises the following steps: (1) cell resuscitation: resuscitating cells from liquid nitrogen; (2) carrying out cell multiplication culture: carrying out step-by-step amplification multiplication culture in T bottles and cell culture bags with different specifications; (3) large-scale culture of cells: performing large-scale culture by using a bioreactor; and (4) harvesting the cells: collecting the cell suspension, and centrifugally harvesting the cells. The preparation method overcomes the problem that a large number of thymus cells or human lymphocytes need to be used as immunogens when the anti-human T lymphocyte immune globulin is prepared, can simply and quickly prepare a large number of Jurkat cells, is used for preparing the anti-human T lymphocyte immune globulin, improves the quality, reduces the biological safety risk, and has a wide application prospect. The method is beneficial to industrial production and has a relatively good economic effect.

The present invention relates to modified human lymphocytes comprising and expressing a recombinant nucleic acid or set of recombinant nucleic acids encoding at least one positive autophagy regulator. The modified human lymphocytes according to the present invention may also comprise and express recombinant nucleic acids or groups of recombinant nucleic acids encoding T cell receptors or chimeric antigen receptors. The invention also relates to methods of producing such modified human lymphocytes, pharmaceutical compositions comprising them, and their use in medicine and for methods of treatment of cancer, including immunotherapeutic treatment of cancer.

The present application relates to the technical field of medical quality control products, in particular to a quality control substance for lymphocyte subset analysis and a preparation method thereof, comprising human monocyte simulation particles, human granulocyte simulation particles, human lymphocyte simulation particles, human red blood cell simulation particles, human platelet simulation particles and a preservative, wherein the human monocyte simulation particles, the human granulocyte simulation particles, the human lymphocyte simulation particles, the human red blood cell simulation particles and the human platelet simulation particles are added into the preservative, and mixed according to the concentration of human particles to obtain the quality control substance for lymphocyte subset analysis. Compared with the prior art, the quality control substance for lymphocyte subset analysis and the preparation method thereof have the advantages of simple operation, small batch difference and good stability, and can be applied to the field of quality control of flow cytometry methodological lymphocyte subset analysis.

The present invention relates to a specific type of non-porous and non-magnetic composite material consisting of a solid surface having a specific diameter and an antigen recognition element for use in a method for activating and amplifying T lymphocytes.

The present disclosure relates to in vitro mammalian, e.g., human, lymphoid cell cultures and systems, and the utility of these culture and systems for determining the immunogenicity of candidate therapeutic molecules. The present disclosure also relates to in vitro methods of antibody production utilizing the disclosed lymphoid cell cultures.

Provided are a human B lymphocyte stimulator receptor (TACI) antibody having high specificity and high affinity for TACI, and a method for detecting TACI or a TACI-Fc fusion protein by using the TACI antibody. By means of the method, the content of a free TACI or TACI-Fc fusion protein drug in a patient can be detected, and the level of drug absorption in the patient is effectively known according to the content of the drug in the patient, thereby achieving the effects of evaluating the illness state, monitoring the efficacy, and determining the prognosis of a patient with an autoimmune disease.

An antigen peptide is provided specifically for treating individuals suffering from ovarian cancer, preferably based on BRCA1 gene c. 5470_5477del8 mutation. It is selected from amino acid sequences of SEQ ID NO. 1 to SEQ ID NO. 6, or derived by substitution, deletion and / or addition of at least one amino acid. The neoantigen polypeptides of the present disclosure can significantly activate T lymphocytes specific to the BRCA1 gene c. 5470_5477del8 mutation in vitro, stimulating the release of the cytokine IFN-y, indicating notable immunogenicity. This enhances T lymphocytes' ability to target and kill cancer cells from ovarian cancer patients carrying the BRCA1 gene c. 5470_5477del8 mutation. Additionally, the antigen peptide can activate and expand human T lymphocytes specific to the BRCA1 gene c. 5470_5477del8 mutation in vitro for adoptive cell therapy. The antigen peptide of the present disclosure addresses the gap in personalized antigen peptide therapies for ovarian cancer patients with BRCAl-c. 5470_5477del8 somatic mutations.

The present invention provides stable pharmaceutical formulations comprising a human antibody that specifically binds to human Lymphocyte-Activation Gene-3 (LAG-3). In certain embodiments, the formulations comprise, in addition to the anti-LAG-3 antibody, a buffer, an amino acid, a non-ionic surfactant, and a sugar. The pharmaceutical formulations of the present invention exhibit a considerable degree of antibody stability upon stress and storage.

The invention relates to a lymphocyte simulation composition, a preparation method, a quality control substance and a quality control method. The lymphocyte simulation composition comprises lymphocyte simulation particles stored in a preservation solution, the volume ratio of the lymphocyte simulation particles in the composition is 50% or above, the lymphocyte simulation particles are obtained by treating lymphocytes separated from blood of mammals, and the lymphocyte simulation particles are used for simulating the lymphocytes. When the lymphocyte simulation particle is tested by a hematology analyzer, the lymphocyte simulation particle has the same or similar light scattering characteristics as human lymphocytes, and the light scattering characteristics at least reflect the complexity of cell contents. By independently preparing the lymphocyte simulation particles, the particles have good consistency and stability, and the obtained composition has small batch-to-batch difference when serving as a quality control substance, so that the quality control of a hematology analyzer is facilitated, and the reliability of clinical examination of the hematology analyzer is improved.

The application discloses a kind of containing isoindole ketone spleen tyrosine kinase inhibitor and its preparation method and application, belong to the technical field of medicinal chemistry.The application uses active group splicing method, retains the trimethoxy phenyl and pyrimidine of Fostamatinib structure as parent nucleus, replaces pyridoxylidene oxazinone with isoindole ketone, designs and synthesized 16 target compounds (general formula is shown below), and test the antitumor, antibacterial and anti-inflammatory activity of the compound, wherein, compound 6a is superior to positive control drug on the inhibition of human B lymphocyte (Raji), 6l on human breast cancer cell (MCF-7) and 6j human hepatoma cell (HepG2);Compound 6l has the best antibacterial activity, 6i and 6j are better than positive control drug Fostamatinib in anti-inflammatory activity, therefore, the isoindole ketone-containing spleen tyrosine kinase inhibitor of the application has good development prospect.