High efficiency fetal calf serum substitute

Abstract

The invention relates to a high efficiency fetal calf serum substitute. The high efficiency fetal calf serum substitute is composed of recombinant human albumin, recombinant human transferrin, galactose, lipid, a trace element compound and vitamins, a weight ratio of the recombinant human albumin to the recombinant human transferrin to the galactose is 2:50:(15-25), and the lipid is a cholesterol, linoleic acid and linolenic acid mixture. The fetal calf serum substitute provided by the invention avoids infectious disease propagation risk and foreign protein induced allergy, increases the in vitro culture survival rate and the increment multiple of human lymphocytes to 96% and 28 respectively, improves the supply of the lymphocytes, reduces the culture cost, and facilitates large scale use.

Description

technical field [0001] The invention relates to a serum substitute, in particular to a high-efficiency fetal bovine serum substitute. Background technique [0002] The medium provides basic environmental and nutrient factors for cell growth, including osmotic pressure, pH value, inorganic salts, amino acids, functional proteins, vitamins, etc. In order to provide these nutritional factors, traditional culture media mostly use animal or human serum and its components as additives. The more common ones include newborn bovine serum, fetal bovine serum, human AB serum, bovine serum albumin, and human serum albumin. , human transferrin, etc. Cellular immunotherapy is inseparable from the cultivation of a large number of lymphocytes in vitro, and the cultivation of lymphocytes requires a large amount of culture medium. The advantages of using animal serum are sufficient supply and low price, but the disadvantage of using animal serum is the potential transmission of infection ri...

AI Technical Summary

Problems solved by technology

Cellular immunotherapy is inseparable from the cultivation of a large number of lymphocytes in vitro, and the cultivation of lymphocytes requires a large amount of culture medium. The advantages of using animal serum are sufficient supply and low price, but the disadvantage of using animal serum is the potential transmission of infection risk of disease, and the development of allergies due to foreign proteins

[0003] CN102352343A (published on February 15, 2012) discloses a lymphocyte culture medium, including a basal medium and a serum substitute, and the serum substitute includes recombinant human serum albumin, recombinant human transferrin, recombinant human insulin , lipids, trace element compounds and vitamins to solve the problem that most of the existing lymphocyte culture media use animal or human serum and its components as additives, which increases the risk of spreading diseases

[0004] Although the above-mentioned prior art discloses some fetal bovine serum substitutes, which can meet certain needs, there are still certain defects in these: for example, the survival rate of lymphocytes cultured in vitro is only about 92%, and the value-added multiple is only about 22, which is obviously low. in the control group, which would affect the widespread use of the above-mentioned serum replacement