This invention discloses a method for the bioenzymatic synthesis of ascorbate
phosphate succinyl
tripeptide-1, belonging to the field of bioengineering technology. This method involves constructing a
fusion protein expression
system through
genetic engineering, releasing the target
tripeptide GHK (glycylhistyllysine) through specific enzymatic cleavage, and then conjugating it to the succinyl group of ascorbate
phosphate via an enzymatic catalytic
esterification reaction, ultimately obtaining high-purity ascorbate
phosphate succinyl
tripeptide-1. Compared with traditional
chemical synthesis methods, this method has advantages such as shorter synthesis steps, milder conditions, environmental friendliness, and high yield (>85%), making it suitable for large-scale industrial production and showing broad application prospects in the
cosmetics and pharmaceutical fields.