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Serum substitute for cell culture and preparation method thereof, serum substitute composition for cell culture, cell culture medium

A serum substitute and cell culture technology, which is applied in the field of serum substitute composition for cell culture, cell culture medium, serum substitute for cell culture and its preparation, can solve the problem of lack of serum-free medium and reduce the difference in results As well as the probability of being infected by viruses and mycoplasma, the effect of excellent effect, high cell density and cell survival rate

Active Publication Date: 2021-08-24
河南普诺易生物制品研究院有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the above-mentioned serum-free medium is only suitable for the cultivation of HeLa cells and is highly targeted.
There is currently no universal serum-free medium suitable for all types of cells

Method used

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  • Serum substitute for cell culture and preparation method thereof, serum substitute composition for cell culture, cell culture medium
  • Serum substitute for cell culture and preparation method thereof, serum substitute composition for cell culture, cell culture medium
  • Serum substitute for cell culture and preparation method thereof, serum substitute composition for cell culture, cell culture medium

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preparation example Construction

[0037] Culturing cells of the invention is prepared by serum replacement, comprising the steps of: dissolving the components in water and then sterilized by filtration, to obtain.

[0038] Preferably, each component comprising first dissolved in water to 450 ~ 550mL water and dissolved, then volume. First the components were dissolved in water and sufficiently dissolved facilitate mixing of the components.

Embodiment 1

[0041] Cell culture according to the present embodiment with a serum replacement component and the water content of the mass of the composition: per liter of cell culture in the serum replacement is an amino acid 18.5mg, protein 203mg, inorganic salt is 62.9mg, ethanolamine 1mg, sodium pyruvate 50mg, ascorbyl phosphate was 1.25mg, glutathione is 0.5mg, insulin 5mg.

[0042] The amino acid alanine 2.25mg, 3.5mg asparagine, 3.25mg aspartic acid, 3.5mg 6mg proline and glutamic acid; salts of 0.43mg ZnSO 4 · 7h 2 O, 0.00015mg ammonium metavanadate, 0.000625mg sulfate, 0.000025mg manganese chloride, 0.0025mg sodium selenite and 62.5mg Na 2 HPO 4 · H 2 O composition; 200mg of protein albumin, 3mg transferrin composition.

[0043] Cell culture according to the present embodiment is a serum replacement first formulated as 10-fold concentrated solution was diluted 10 times with water and then used.

Embodiment 2

[0045] Cell culture according to the present embodiment with a serum replacement component and the water content of the mass of the composition: per liter of cell culture in serum replacement amino acid is 67mg, the protein is 815mg, the inorganic salt is 251.8mg, ethanolamine 3.8mg, sodium pyruvate 200mg, ascorbyl phosphate is 5mg, glutathione is 2mg, insulin 20mg.

[0046] 9mg by the amino acid alanine, 15mg asparagine, 13mg aspartic acid, glutamic acid 15mg, 15mg proline; salts of 1.75mg ZnSO 4 · 7h 2 O, 0.0006mg of ammonium metavanadate, 0.0025mg sulfate, 0.00010mg manganese chloride, 0.01mg sodium selenite, 250mg Na 2 HPO 4 · H 2 O composition; 800mg of protein albumin, 15mg transferrin composition.

[0047] Cell culture according to the present embodiment is a serum replacement first formulated as 10-fold concentrated solution was diluted 10 times with water and then used.

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Abstract

The invention belongs to the technical field of cell culture, and in particular relates to a serum substitute for cell culture and a preparation method thereof, a serum substitute composition for cell culture and a cell culture medium. The serum substitute for cell culture of the present invention includes water and the following components in parts by weight: 18.5-67 parts of amino acid, 200-815 parts of protein, 62.5-252 parts of inorganic salt, 1.0-3.8 parts of ethanolamine, 50-50 parts of sodium pyruvate 200.0 parts, 1.25-5.0 parts of ascorbyl phosphate, 0.5-2.0 parts of glutathione, 5.0-20 parts of insulin. The serum substitute for cell culture of the present invention can be added to any cell culture medium, reducing the amount of serum added, thereby saving costs. The serum substitute for cell culture of the invention is suitable for recombinant protein and vaccine production as well as other types of cell culture, and has strong versatility.

Description

Technical field [0001] The present invention belongs to the technical field of cell culture, in particular to a cell culture and with a method for preparing serum replacement, the cells were serum substitute composition, the cell culture medium. Background technique [0002] Development of cell culture technology has gone through a hundred years, it has been widely used in research biology and medicine. The characteristics of the cells, whether attached to the support growth of cells into attachment type, suspension type cells. Cell culture media supply both in cultured cells during cell nutrition and the promotion of basic reproductive material of the cell proliferation, but also cell growth and reproduction of the living environment. Animal cell culture is generally necessary to add 10 to 15% (volume fraction) fetal bovine serum (FBS), but the increased costs expensive serum and serum viral easily and infection, there is a difference in the quality of different batches of serum...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/07
CPCC12N5/0018C12N2500/12C12N2500/20C12N2500/22C12N2500/24C12N2500/25C12N2500/32C12N2500/38C12N2500/46C12N2501/998C12N2501/999
Inventor 王建华杨献军林钟超
Owner 河南普诺易生物制品研究院有限公司
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