Method for converting in-vitro activated adipose-derived stem cells into chondroblasts

Abstract

The invention discloses a method for converting in-vitro activated adipose-derived stem cells into chondroblasts. The method comprises the following steps of preparing a DMEM culture medium, wherein the DMEM culture medium is prepared from 1% of fetal calf serum, 10 ng / ml of TGF-beta, 100 ng / ml of BMP-6, 6.25 mu g / ml of insulin, 0.1 mu mol / L of dexamethasone, 6.25 mu g / ml of transferrin and 50 mumol / L of ascorbyl phosphate; culturing the adipose-derived stem cells formed into micelles in the DMEM culture medium; and injecting the adipose-derived stem cells cultured for 7-9 days into an articular cavity and converting the adipose-derived stem cells into chondroblasts. The method has the advantages of time saving, simple process control and low rejection of the generated chondroblasts.

Description

technical field [0001] The invention relates to the field of medicine, in particular to a method for activating adipose stem cells in vitro to transform into prochondrocytes. Background technique [0002] Among the mesenchymal stem cells (MSCs) that have been used more so far, bone marrow derived stem cells (BMSCs) have been a research hotspot and have been widely used in scientific research. In 2001, Zuk et al. isolated for the first time multidirectionally differentiated stem cells from adipose tissue suspension extracted by liposuction. Adipose-derived mesenchymal cells (ASCs) are superior to BMSCs because stem cells extracted from adipose tissue have the advantages of easy sampling, large amount of sampling, repeated sampling and rapid cell proliferation. At the same time, ASCs can differentiate into chondrocytes, osteoblasts, adipocytes, nerve cells, etc. in a specific microenvironment. Therefore, ASCs are expected to be good seed cells for tissue engineering. [000...

AI Technical Summary

Problems solved by technology

[0003] The main problems are: (1) There are many types of ASCs surface molecules known at present, but most of them cross with other mesenchymal stem cells, and there is no clear specific surface antigen, so it is difficult to select one of the highly specific surface marker antigens Combined screening; (2) Although many laboratories try to use different materials and techniques to induce ASCs to differentiate into cartilage tissue, they cannot avoid the result of inflammatory reaction between cartilage tissues; (3) In many experimental cultured chondrocytes, the late The experimental results all showed the hypertrophy and hyperplasia of chondrocytes; (4) At present, the induction of ASCs to differentiate into chondrocytes basically adopts the construction of 3D models, but the clinical practicability of this method is not high