The invention discloses an IL-2 and MART-1 dual-gene co-expression recombinant vector, which is sequentially linked with an IL-2 gene, an IRES sequence and an MART-1 gene in a vector transcription direction, or is sequentially linked with an MART-1 gene, an IRES sequence and an IL-2 gene in a vector transcription direction, wherein nucleotide sequence of the IL-2 gene is represented in SEQ ID NO: 1 in a sequence list, nucleotide sequence of the MART-1 gene is represented in SEQ ID NO: 2 in the sequence list, and nucleotide sequence of the IRES sequence is represented in SEQ ID NO: 3 in the sequence list. The dual-gene co-expression recombinant vector, which links the IL-2 gene and the MART-1 gene through the IRES sequence, can simultaneously express a human melanin differentiation antigen and interleukin-2; the recombinant vector, in immunogene therapy of melanin, not only can develop an immune regulating function of a cell factor but also can generate a specific anti-tumor effect aiming at the melanin.