Filtering membrane pretreatment liquid for improving immunofluorescence detection sensitivity and preparation method and application thereof

A technology of immunofluorescence detection and pretreatment solution, which is applied in the direction of fluorescence/phosphorescence, chemical instruments and methods, and laboratory containers, which can solve the problems of poor detection sensitivity, reduce non-specific adsorption, improve detection sensitivity, The effect of enhancing reaction sensitivity

Pending Publication Date: 2021-11-02
重庆创芯生物科技有限公司
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Problems solved by technology

Preliminary research in the laboratory has shown that the pretreatment formula of the filter membrane plays a key role in the detection sensitivity of the project. For the detection of items with a high detection threshold such as MYO, ordinary filter membra

Method used

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  • Filtering membrane pretreatment liquid for improving immunofluorescence detection sensitivity and preparation method and application thereof
  • Filtering membrane pretreatment liquid for improving immunofluorescence detection sensitivity and preparation method and application thereof
  • Filtering membrane pretreatment liquid for improving immunofluorescence detection sensitivity and preparation method and application thereof

Examples

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Embodiment 1-7

[0033] The following example 1 is used as an example to illustrate the preparation method of the filter membrane pretreatment solution for improving the sensitivity of immunofluorescence detection, comprising the following steps:

[0034] (1) preparation concentration is the buffer solution Tris-HCL of 50Mm / L, adjusts pH value with the hydrochloric acid of 4M / L;

[0035] (2) Weigh the surfactant PVP, PEG6000, sugar, inert protein, heterophagic antibody, anti-erythrocyte antibody in a clean beaker;

[0036] (3) Then add the buffer solution Tris-HCL into the beaker of step (2), stir on a magnetic stirrer at a stirring speed of 300-500rpm for 20min until all the substances are dissolved, and continue stirring for 10min after the volume is set to 100ml.

[0037] Refer to Table 1, Table 2 and Table 3 for the values ​​of solvent concentration, solvent pH value and each solute concentration of Examples 1-6.

[0038] Table 1 is the solvent concentration, solvent pH value and each sol...

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Abstract

The invention discloses a filtering membrane pretreatment liquid for improving immunofluorescence detection sensitivity and a preparation method and application thereof. The filtering membrane pretreatment liquid for improving the immunofluorescence detection sensitivity comprises a solvent and solute, the solvent is a buffer solution Tris-HCL, the concentration of the Tris-HCL is 50-100 Mm/L, the solute comprises 2-6 g/L of a surfactant, 1-5 g/L of PVP, 3-6 g/L of PEG6000, 20-60 g/L of saccharides, 30-60 g/L of inert protein, 1-5 mg/L of heterophagy antibody, 1-5 mg/L of anti-erythrocyte antibody and 0.5-1 g/L of preservative. The filter membrane pretreatment liquid has the advantage of improving the detection sensitivity of the micro-fluidic chip.

Description

technical field [0001] The invention belongs to the technical field of microfluidic immunofluorescence detection, more specifically, it relates to a filter membrane pretreatment solution for improving the sensitivity of immunofluorescence detection and its preparation method and application. Background technique [0002] Microfluidic technology is a science and technology characterized by the manipulation of fluids in the micron-scale space, and the core of its technology is the microfluidic chip. Microfluidic chips have the advantages of fast detection speed, high throughput, high sensitivity, and less reagent consumption, and have developed rapidly in recent years. In the 1990s, A.Mans et al proposed the concept of a micro-total analysis system (microfluidic chip) using a chip. In this system, the functions of sample injection, pretreatment, chemical reaction, separation and detection are realized. Miniaturization, automation and integration of equipment. [0003] The de...

Claims

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Application Information

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IPC IPC(8): G01N21/64G01N33/533G01N33/537B01L3/00
CPCG01N21/6428G01N33/533G01N33/537B01L3/00G01N2021/6439
Inventor 唐海燕钟越杨燕斌于梦露赵朝辉
Owner 重庆创芯生物科技有限公司
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