Filtering membrane pretreatment liquid for improving immunofluorescence detection sensitivity and preparation method and application thereof

Abstract

The invention discloses a filtering membrane pretreatment liquid for improving immunofluorescence detection sensitivity and a preparation method and application thereof. The filtering membrane pretreatment liquid for improving the immunofluorescence detection sensitivity comprises a solvent and solute, the solvent is a buffer solution Tris-HCL, the concentration of the Tris-HCL is 50-100 Mm / L, the solute comprises 2-6 g / L of a surfactant, 1-5 g / L of PVP, 3-6 g / L of PEG6000, 20-60 g / L of saccharides, 30-60 g / L of inert protein, 1-5 mg / L of heterophagy antibody, 1-5 mg / L of anti-erythrocyte antibody and 0.5-1 g / L of preservative. The filter membrane pretreatment liquid has the advantage of improving the detection sensitivity of the micro-fluidic chip.

Description

technical field [0001] The invention belongs to the technical field of microfluidic immunofluorescence detection, more specifically, it relates to a filter membrane pretreatment solution for improving the sensitivity of immunofluorescence detection and its preparation method and application. Background technique [0002] Microfluidic technology is a science and technology characterized by the manipulation of fluids in the micron-scale space, and the core of its technology is the microfluidic chip. Microfluidic chips have the advantages of fast detection speed, high throughput, high sensitivity, and less reagent consumption, and have developed rapidly in recent years. In the 1990s, A.Mans et al proposed the concept of a micro-total analysis system (microfluidic chip) using a chip. In this system, the functions of sample injection, pretreatment, chemical reaction, separation and detection are realized. Miniaturization, automation and integration of equipment. [0003] The de...

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Problems solved by technology

Preliminary research in the laboratory has shown that the pretreatment formula of the filter membrane plays a key role in the detection sensitivity of the project. For the detection of items with a high detection threshold such as MYO, ordinary filter membranes can only meet the requirements by optimizing the cross-linking system and other methods. However, it is not good to use ordinary filter membranes for items with high detection sensitivity. Therefore, it is very important to develop its treatment formula

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