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Immunochromatographic test strip and device comprising the same

An immunochromatography test paper and coating technology, which is applied in the field of immunoassay, can solve problems such as difficulty, hindering the flow of labeled antibody chromatography, and affecting the reaction.

Active Publication Date: 2020-10-16
北京康思润业生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In this method, after the whole blood sample is added to the sample pad, the reaction time between the blood cells and the RBC antibody is very short, and it is difficult to effectively react with a single RBC antibody to form a cross-linked polymer. Therefore, in this case, it has not yet been used. The blood cells that fully react to form polymers can easily be chromatographed on the NC membrane (nitrocellulose membrane) through the sample pad, causing the NC membrane to be clogged, hindering the formation of the test complex between the labeled antibody and the sample to be tested to continue the chromatographic flow, affecting the reaction

Method used

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  • Immunochromatographic test strip and device comprising the same
  • Immunochromatographic test strip and device comprising the same
  • Immunochromatographic test strip and device comprising the same

Examples

Experimental program
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Embodiment 1

[0062] Example 1 Preparation of immunochromatographic test strips for detecting soluble growth-stimulating expression gene 2 protein (ST2)

[0063] 1.1 Preparation of labeled antibody

[0064] (1) Take 50 μL of carboxy fluorescent latex microsphere solution (20 mg / mL), add 450 μL of phosphate buffer (10 mM, pH 6.0) and mix well;

[0065] (2) Take 5 μL, 500 mg / mL of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) solution, and add it to the carboxy fluorescent latex microsphere solution diluted in step (1) (the weight ratio of the carboxyl fluorescent latex microspheres and EDC is 1:2.5), mixed uniformly, and reacted at room temperature for 30 minutes;

[0066] (3) The carboxyl fluorescent latex microsphere solution activated in step (2) was centrifuged and washed with phosphate buffer (10 mM, pH 6.0) at a rotation speed of 14000 rpm for 9 min. The supernatant was discarded and repeated twice.

[0067] (4) Add 500 μL of phosphate buffer (10 mM, pH 6.0) to the precipitate...

Embodiment 2

[0095] Example 2 Preparation of immunochromatographic test strips for detecting soluble growth-stimulating expression gene 2 protein (ST2)

[0096] 2.1 Preparation of labeled antibody

[0097] 1.1 with embodiment 1.

[0098] 2.2 Preparation of marker pads

[0099] 1.2 with embodiment 1.

[0100] 2.3 Preparation of coated pad

[0101] 1.3 with embodiment 1.

[0102] 2.4 Sample pad preparation

[0103] RBC complex preparation

[0104] (1) Take 50 μL of white carboxyl latex microspheres (20 mg / mL), add 450 μL of phosphate buffer (10 mM, pH 6.0) and mix well;

[0105] (2) Take 5 μL, 500mg / mL of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) solution, add the white carboxyl latex microsphere solution diluted in step (1) , mixed evenly, and reacted at room temperature for 30 minutes; the weight ratio of the white carboxyl latex microspheres to EDC was 1:2.5;

[0106] (3) Centrifuge the white carboxyl latex microspheres activated in step (2) with phosphate buffer (10mM,...

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Abstract

The present invention relates to an immunochromatographic test strip, which comprises: a sample pad; a marker pad, which is connected to the sample pad; a coating pad, which is connected to the markerpad, and is equipped with a detection line and a quality control line sequentially; and a water absorption pad, which is connected to the coating pad; wherein the sample pad comprises a glass fiber pad, and an RBC antibody compound is sprayed on the glass fiber pad. The invention also provides a device comprising the immunochromatographic test strip. The invention relates to the immunochromatographic test strip and the device comprising the same, an RBC antibody in the RBC antibody compound and red blood cells are subjected to immunoaffinity reaction to form a cross-linked substance of "red blood cell-RBC antibody compound-red blood cell", compared with a single RBC antibody, the RBC compound is larger in size and more in red blood cell binding sites, so that the RBC compound is easier tobind with red blood cells and is intercepted on the sample pad.

Description

technical field [0001] The invention relates to an immunochromatographic test strip and a device including the same, belonging to the technical field of immunoassay. Background technique [0002] The immunological lateral chromatography detection reagent has the advantages of being convenient and quick to use. However, due to the need to meet the requirements for the convenience of the operator, when the fluorescent immunological lateral flow detection reagent is used, it cannot have a relatively cumbersome sample pretreatment process. It can be well applied when detecting clinical samples such as serum and plasma, but for detecting whole blood samples, in order to avoid the influence of blood cells in the sample, it needs to remove the blood cells in the sample before the reaction. [0003] There are two main methods for removing blood cells in whole blood by current immunological lateral flow chromatography: 1. The sample pad is a whole blood filter pad. Because it is sof...

Claims

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Application Information

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IPC IPC(8): G01N33/558G01N33/58
CPCG01N33/558G01N33/585Y02A50/30
Inventor 张林松李海燕孟凡斌
Owner 北京康思润业生物技术有限公司
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