A test paper suitable for quantitative detection of peripheral blood immunochromatography and its application

Abstract

The invention discloses a test paper suitable for the quantitative detection of peripheral blood immunochromatography and its application, comprising a bottom plate, and the bottom plate is sequentially provided with a sample pad, a sealing pad, a reaction pad and a water-absorbing pad along the length direction of the bottom plate; it is characterized in that : the sample pad is coated with a first antibody, the first antibody has a tracer marker and can recognize an antigenic epitope of the target to be tested; the blocking pad is coated with an anti-erythrocyte antibody, and the blocking pad A pad is partially laminated to the reaction pad. The present invention also includes the application of the above-mentioned test paper in the preparation of the immunochromatography detection box or in the quantitative detection of the peripheral blood immunochromatography. Compared with the prior art, the test paper provided by the invention has the advantages of high sensitivity, wide linear range and the like.

Description

technical field [0001] The invention relates to the technical field of medical detection, in particular to a test paper suitable for quantitative detection of peripheral blood immune chromatography and its application. Background technique [0002] Immu-nochromatography assay (ICA) is a new type of immunoassay that appeared in the early 1980s. It is a simple and rapid immunological detection technology based on immunodiafiltration. The principle of immunochromatography is to immobilize specific antibodies on a certain zone of the nitrocellulose membrane. When one end of the dry nitrocellulose is immersed in the sample, the sample will move forward along the membrane due to the action of the capillary. When moving to the area where the antibody is immobilized, the corresponding antigen in the sample will specifically bind to the antibody. If stained with immunocolloidal gold or immunoenzyme, the area can display a certain color, thereby achieving specific immunodiagnosis. Th...

AI Technical Summary

Problems solved by technology

Because the amount of blood sampled from peripheral blood is usually less, it is difficult to meet the minimum sample size required by PCT or NT-proBNP level test strips in the prior art. There will be greater interference in the detection, and the existence of these problems makes it difficult to accurately detect the true content of markers such as PCT or NT-proBNP in the peripheral blood using the detection test strips in the prior art

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